Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Pseudomonas straminea | |
cysteine | - |
Pseudomonas straminea | |
DTT | - |
Pseudomonas straminea | |
reduced glutathione | - |
Pseudomonas straminea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoCl2 | - |
Pseudomonas straminea | |
CuSO4 | - |
Pseudomonas straminea | |
HgCl2 | - |
Pseudomonas straminea | |
iodoacetamide | weak | Pseudomonas straminea | |
NEM | - |
Pseudomonas straminea | |
NiCl2 | - |
Pseudomonas straminea | |
PCMB | - |
Pseudomonas straminea | |
ZnCl2 | - |
Pseudomonas straminea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0064 | - |
(E)-3-Carboxy-5-oxo-2-hex-2-enedioic acid | pH 8.0, 24°C | Pseudomonas straminea | |
0.014 | - |
2-hydroxy-4-oxobutane-1,2,4-tricarboxylate | pH 8.0, 24°C | Pseudomonas straminea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no Fe2+- or Mn2+-dependent activation in the presence of DTT under anaerobic conditions | Pseudomonas straminea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, SDS-PAGE | Pseudomonas straminea |
68000 | - |
gel filtration | Pseudomonas straminea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas straminea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas straminea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
108 | - |
- |
Pseudomonas straminea |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 1 month | Pseudomonas straminea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-3-carboxy-5-oxo-2-hex-2-enedioic acid + H2O | i.e. (E)-4-oxobut-1-ene-1,2,4-tricarboxylate, 4-oxalmesaconate, highly specific | Pseudomonas straminea | 2-hydroxy-4-oxobutane-1,2,4-tricarboxylate | i.e. 4-carboxy-4-hydroxy-2-oxoadipic acid, gamma-oxalcitramalate | r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 37000, SDS-PAGE | Pseudomonas straminea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
assay at | Pseudomonas straminea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
- |
Pseudomonas straminea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 9.6 | 50% of activity maximum at pH 7.2 and 9.6 | Pseudomonas straminea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas straminea | isoelectric focusing | - |
4.9 |