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Literature summary for 4.2.1.82 extracted from

  • Rahman, M.M.; Andberg, M.; Koivula, A.; Rouvinen, J.; Hakulinen, N.
    The crystal structure of D-xylonate dehydratase reveals functional features of enzymes from the Ilv/ED dehydratase family (2018), Sci. Rep., 8, 865 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expressed in Escherichia coli Caulobacter vibrioides

Crystallization (Commentary)

Crystallization (Comment) Organism
the crystal structure of D-xylonate dehydratase from Caulobacter crescentus is described at 2.7 A resolution and it is compares with other available enzyme structures from the IlvD/EDD protein family Caulobacter vibrioides

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ each monomer is composed of two domains in which the N-terminal domain forms a binding site for a [2Fe-2S] cluster and a Mg2+ ion Caulobacter vibrioides

Organism

Organism UniProt Comment Textmining
Caulobacter vibrioides Q9A9Z2
-
-
Caulobacter vibrioides ATCC 19089 Q9A9Z2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Caulobacter vibrioides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylonate
-
Caulobacter vibrioides 2-dehydro-3-deoxy-D-arabinonate + H2O
-
?
D-xylonate
-
Caulobacter vibrioides ATCC 19089 2-dehydro-3-deoxy-D-arabinonate + H2O
-
?

Subunits

Subunits Comment Organism
tetramer crystallographic data, each monomer is composed of two domains in which the N-terminal domain forms a binding site for a [2Fe-2S] cluster and a Mg2+ ion Caulobacter vibrioides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Caulobacter vibrioides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Caulobacter vibrioides

Cofactor

Cofactor Comment Organism Structure
[2Fe-2S]-center each monomer is composed of two domains in which the N-terminal domain forms a binding site for a [2Fe-2S] cluster and a Mg2+ ion Caulobacter vibrioides