BRENDA - Enzyme Database show
show all sequences of 4.2.1.80

Metabolism of allylglycine and cis-crotylglycine by Pseudomonas putida (arvilla) mt-2 habouring a TOL plasmid

Kunz, D.A.; Ribbons, D.W.; Chapman, P.J.; J. Bacteriol. 148, 72-82 (1981)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
TOL plasmid-haboring
-
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
TOL plasmid-haboring
-
Pseudomonas sp.
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.33
0.54
cell-free extracts from succinate-grown cells, strain-dependent
Pseudomonas putida
5.2
-
cell-free extracts from DL-allylglycerine grown cells
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-keto-cis-4-hexenoic acid
50% the rate of 4-hydroxy-2-oxopentanoate, higher activity towards cis than towards trans-isomer
648397
Pseudomonas putida
?
-
-
-
?
2-keto-cis-4-hexenoic acid
50% the rate of 4-hydroxy-2-oxopentanoate, higher activity towards cis than towards trans-isomer
648397
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
?
-
-
-
?
4-hydroxy-2-oxopentanoate
-
648397
Pseudomonas sp.
2-oxopent-4-enoate + H2O
-
-
-
?
4-hydroxy-2-oxopentanoate
-
648397
Pseudomonas putida
2-oxopent-4-enoate + H2O
-
-
-
?
4-hydroxy-2-oxopentanoate
-
648397
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
2-oxopent-4-enoate + H2O
-
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.33
0.54
cell-free extracts from succinate-grown cells, strain-dependent
Pseudomonas putida
5.2
-
cell-free extracts from DL-allylglycerine grown cells
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-keto-cis-4-hexenoic acid
50% the rate of 4-hydroxy-2-oxopentanoate, higher activity towards cis than towards trans-isomer
648397
Pseudomonas putida
?
-
-
-
?
2-keto-cis-4-hexenoic acid
50% the rate of 4-hydroxy-2-oxopentanoate, higher activity towards cis than towards trans-isomer
648397
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
?
-
-
-
?
4-hydroxy-2-oxopentanoate
-
648397
Pseudomonas sp.
2-oxopent-4-enoate + H2O
-
-
-
?
4-hydroxy-2-oxopentanoate
-
648397
Pseudomonas putida
2-oxopent-4-enoate + H2O
-
-
-
?
4-hydroxy-2-oxopentanoate
-
648397
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
2-oxopent-4-enoate + H2O
-
-
-
?
Other publictions for EC 4.2.1.80
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747105
Johnson
Stereochemical consequences o ...
Leptothrix cholodnii, Leptothrix cholodnii SP-6, Pseudomonas putida
Biochemistry
55
4055-4064
2016
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6
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2
2
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705177
Montgomery
Assembly of a 20-nm protein ca ...
Escherichia coli K-12
J. Mol. Biol.
396
1379-1391
2010
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1
1
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3
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2
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1
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3
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1
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1
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672682
Orii
Metabolism of 4-amino-3-hydrox ...
Bordetella sp. 10d, Bordetella sp.
Biosci. Biotechnol. Biochem.
70
2653-2661
2006
-
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-
-
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1
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1
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661714
Wang
Determination of the metal ion ...
Paraburkholderia xenovorans
FEBS J.
272
966-974
2005
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-
1
-
-
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-
4
-
5
2
1
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1
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1
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4
1
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4
1
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1
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4
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5
2
1
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1
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4
1
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4
1
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648402
Lim
Characterization of the pcbE g ...
Pseudomonas sp., Pseudomonas sp. DJ-12
Arch. Pharm. Res.
23
187-195
2000
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-
1
-
-
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1
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6
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648399
Romine
-
Complete sequence of a 184-kil ...
Novosphingobium aromaticivorans, Novosphingobium aromaticivorans F199
J. Bacteriol.
181
1282-1602
1999
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2
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648401
Pollard
Purification, characterization ...
Escherichia coli
Eur. J. Biochem.
251
98-106
1998
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1
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2
1
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1
1
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2
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1
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1
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1
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1
1
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1
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1
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648400
Kim
Localization and sequence anal ...
Pseudomonas sp., Pseudomonas sp. DJ77
Biochem. Biophys. Res. Commun.
238
56-60
1997
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1
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6
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648398
Harayama
-
Physically associated enzymes ...
Pseudomonas putida, Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
J. Bacteriol.
171
6221-6228
1989
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1
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1
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19
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1
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2
1
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1
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648397
Kunz
Metabolism of allylglycine and ...
Pseudomonas putida, Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182, Pseudomonas sp.
J. Bacteriol.
148
72-82
1981
-
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20
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1
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5
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