BRENDA - Enzyme Database show
show all sequences of 4.2.1.79

The PrpF protein of Shewanella oneidensis MR-1 catalyzes the isomerization of 2-methyl-cis-aconitate during the catabolism of propionate via the AcnD-dependent 2-methylcitric acid cycle

Rocco, C.J.; Wetterhorn, K.M.; Garvey, G.S.; Rayment, I.; Escalante-Semerena, J.C.; PLoS ONE 12, e0188130 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene mmgE, the mother cell metabolic gene (mmg) operon encodes homologues from the methylcitric acid cycle
Bacillus subtilis
gene prpD, the mother cell metabolic gene (mmg) operon encodes homologues from the methylcitric acid cycle
Escherichia coli
gene prpD, the sequence of prp genes is prpR-prpB-prpC-prpD-prpE, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Salmonella enterica subsp. enterica serovar Typhimurium
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of enzyme-deficient DELTA prpD mutant. The two genes acnD and prpF, encoding 2-methylcitrate dehydratase (AcnD, EC 4.2.1.117) and 2-methyl-aconitate isomerase (SoPrpF, EC 5.3.3.-) from Shewanella oneidensis together can replace gene prpD in Salmonella enterica. SoAcnD and SePrpD synthesize different methylaconitate isomers
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
Escherichia coli
-
(Z)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
Bacillus subtilis
-
(Z)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
Salmonella enterica subsp. enterica serovar Typhimurium
-
(Z)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
Bacillus subtilis 168
-
(Z)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720
-
(Z)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
P45859
-
-
Bacillus subtilis 168
P45859
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
P74840
-
-
Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720
P74840
-
-
Escherichia coli
P77243
-
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
-
749113
Escherichia coli
(Z)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
-
749113
Bacillus subtilis
(Z)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
-
749113
Salmonella enterica subsp. enterica serovar Typhimurium
(Z)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
-
749113
Bacillus subtilis 168
(Z)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
-
749113
Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720
(Z)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
-
749113
Escherichia coli
(E)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
-
749113
Bacillus subtilis
(E)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
-
749113
Bacillus subtilis 168
(E)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
-
?
additional information
the MmgE enzyme is not a stereospecific 2-methylcitrate dehydratase because it can dehydrate at least two of the four diastereomers of 2-methylcitrate to yield either (E)-2-methylaconitate or (Z)-2-methylaconitate, but the physiological pathways proceed via (Z)-2-methylaconitate, which serves as the substrate for the citB enzyme in the synthesis of 2-methylisocitrate
749113
Bacillus subtilis
?
-
-
-
-
additional information
the PrpD enzyme from Escherichia coli is not a stereospecific 2-methylcitrate dehydratase because it can dehydrate at least two of the four diastereomers of 2-methylcitrate to yield either (E)-2-methylaconitate or (Z)-2-methylaconitate, but the physiological pathways proceed via (Z)-2-methylaconitate, which serves as the substrate for the citB enzyme in the synthesis of 2-methylisocitrate
749113
Escherichia coli
?
-
-
-
-
additional information
the MmgE enzyme is not a stereospecific 2-methylcitrate dehydratase because it can dehydrate at least two of the four diastereomers of 2-methylcitrate to yield either (E)-2-methylaconitate or (Z)-2-methylaconitate, but the physiological pathways proceed via (Z)-2-methylaconitate, which serves as the substrate for the citB enzyme in the synthesis of 2-methylisocitrate
749113
Bacillus subtilis 168
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
37
assay at
Bacillus subtilis
25
37
assay at
Escherichia coli
25
37
assay at
Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Bacillus subtilis
8
-
assay at
Escherichia coli
8
-
assay at
Salmonella enterica subsp. enterica serovar Typhimurium
Cloned(Commentary) (protein specific)
Commentary
Organism
gene mmgE, the mother cell metabolic gene (mmg) operon encodes homologues from the methylcitric acid cycle
Bacillus subtilis
gene prpD, the mother cell metabolic gene (mmg) operon encodes homologues from the methylcitric acid cycle
Escherichia coli
gene prpD, the sequence of prp genes is prpR-prpB-prpC-prpD-prpE, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Salmonella enterica subsp. enterica serovar Typhimurium
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of enzyme-deficient DELTA prpD mutant. The two genes acnD and prpF, encoding 2-methylcitrate dehydratase (AcnD, EC 4.2.1.117) and 2-methyl-aconitate isomerase (SoPrpF, EC 5.3.3.-) from Shewanella oneidensis together can replace gene prpD in Salmonella enterica. SoAcnD and SePrpD synthesize different methylaconitate isomers
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
Escherichia coli
-
(Z)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
Bacillus subtilis
-
(Z)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
Salmonella enterica subsp. enterica serovar Typhimurium
-
(Z)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
Bacillus subtilis 168
-
(Z)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720
-
(Z)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
-
749113
Escherichia coli
(Z)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
-
749113
Bacillus subtilis
(Z)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
-
749113
Salmonella enterica subsp. enterica serovar Typhimurium
(Z)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
-
749113
Bacillus subtilis 168
(Z)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
-
749113
Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720
(Z)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
-
749113
Escherichia coli
(E)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
-
749113
Bacillus subtilis
(E)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
-
?
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
-
749113
Bacillus subtilis 168
(E)-but-2-ene-1,2,3-tricarboxylate + H2O
-
-
-
?
additional information
the MmgE enzyme is not a stereospecific 2-methylcitrate dehydratase because it can dehydrate at least two of the four diastereomers of 2-methylcitrate to yield either (E)-2-methylaconitate or (Z)-2-methylaconitate, but the physiological pathways proceed via (Z)-2-methylaconitate, which serves as the substrate for the citB enzyme in the synthesis of 2-methylisocitrate
749113
Bacillus subtilis
?
-
-
-
-
additional information
the PrpD enzyme from Escherichia coli is not a stereospecific 2-methylcitrate dehydratase because it can dehydrate at least two of the four diastereomers of 2-methylcitrate to yield either (E)-2-methylaconitate or (Z)-2-methylaconitate, but the physiological pathways proceed via (Z)-2-methylaconitate, which serves as the substrate for the citB enzyme in the synthesis of 2-methylisocitrate
749113
Escherichia coli
?
-
-
-
-
additional information
the MmgE enzyme is not a stereospecific 2-methylcitrate dehydratase because it can dehydrate at least two of the four diastereomers of 2-methylcitrate to yield either (E)-2-methylaconitate or (Z)-2-methylaconitate, but the physiological pathways proceed via (Z)-2-methylaconitate, which serves as the substrate for the citB enzyme in the synthesis of 2-methylisocitrate
749113
Bacillus subtilis 168
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
37
assay at
Bacillus subtilis
25
37
assay at
Escherichia coli
25
37
assay at
Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Bacillus subtilis
8
-
assay at
Escherichia coli
8
-
assay at
Salmonella enterica subsp. enterica serovar Typhimurium
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in the 2-methylcitric acid cycle, overview. The three genes, mmgDE and yqiQ (mmgF), provide three of the five steps of the methylcitric acid cycle, encoded in the mmg operon. The fourth step can be supplied by citB (aconitase), and the fifth probably missing step, the propionyl-CoA synthetase, is probably skipped because the beta-oxidation of methyl-branched fatty acids by the enzymes encoded by mmgABC produce propionyl-CoA. The 2-methylisocitrate produced by CitB is converted to pyruvate and succinate by the enzyme YqiQ(MmgF)
Bacillus subtilis
metabolism
the enzyme is involved in the 2-methylcitric acid cycle, overview
Escherichia coli
metabolism
the enzyme is involved in the 2-methylcitric acid cycle, overview
Salmonella enterica subsp. enterica serovar Typhimurium
additional information
construction of enzyme-deficient DELTA prpD mutant. The two genes acnD and prpF, encoding 2-methylcitrate dehydratase (AcnD, EC 4.2.1.117) and 2-methyl-aconitate isomerase (SoPrpF, EC 5.3.3.-) from Shewanella oneidensis together can replace gene prpD in Salmonella enterica. SoAcnD and SePrpD synthesize different methylaconitate isomers. The SoPrpF protein isomerizes the product of the AcnD reaction into the PrpD product. Mutant SoPrpF variants with substitutions of residues K73 or C107 fail to support growth with propionate as the sole source of carbon and energy
Salmonella enterica subsp. enterica serovar Typhimurium
physiological function
the MmgE enzyme is not a stereospecific 2-methylcitrate dehydratase because it can dehydrate at least two of the four diastereomers of 2-methylcitrate to yield either (E)-2-methylaconitate or (Z)-2-methylaconitate, but the physiological pathways proceed via (Z)-2-methylaconitate, which serves as the substrate for the citB enzyme in the synthesis of 2-methylisocitrate
Bacillus subtilis
physiological function
the PrpD enzyme from Escherichia coli is not a stereospecific 2-methylcitrate dehydratase because it can dehydrate at least two of the four diastereomers of 2-methylcitrate to yield either (E)-2-methylaconitate or (Z)-2-methylaconitate, but the physiological pathways proceed via (Z)-2-methylaconitate, which serves as the substrate for the citB enzyme in the synthesis of 2-methylisocitrate
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in the 2-methylcitric acid cycle, overview. The three genes, mmgDE and yqiQ (mmgF), provide three of the five steps of the methylcitric acid cycle, encoded in the mmg operon. The fourth step can be supplied by citB (aconitase), and the fifth probably missing step, the propionyl-CoA synthetase, is probably skipped because the beta-oxidation of methyl-branched fatty acids by the enzymes encoded by mmgABC produce propionyl-CoA. The 2-methylisocitrate produced by CitB is converted to pyruvate and succinate by the enzyme YqiQ(MmgF)
Bacillus subtilis
metabolism
the enzyme is involved in the 2-methylcitric acid cycle, overview
Escherichia coli
metabolism
the enzyme is involved in the 2-methylcitric acid cycle, overview
Salmonella enterica subsp. enterica serovar Typhimurium
additional information
construction of enzyme-deficient DELTA prpD mutant. The two genes acnD and prpF, encoding 2-methylcitrate dehydratase (AcnD, EC 4.2.1.117) and 2-methyl-aconitate isomerase (SoPrpF, EC 5.3.3.-) from Shewanella oneidensis together can replace gene prpD in Salmonella enterica. SoAcnD and SePrpD synthesize different methylaconitate isomers. The SoPrpF protein isomerizes the product of the AcnD reaction into the PrpD product. Mutant SoPrpF variants with substitutions of residues K73 or C107 fail to support growth with propionate as the sole source of carbon and energy
Salmonella enterica subsp. enterica serovar Typhimurium
physiological function
the MmgE enzyme is not a stereospecific 2-methylcitrate dehydratase because it can dehydrate at least two of the four diastereomers of 2-methylcitrate to yield either (E)-2-methylaconitate or (Z)-2-methylaconitate, but the physiological pathways proceed via (Z)-2-methylaconitate, which serves as the substrate for the citB enzyme in the synthesis of 2-methylisocitrate
Bacillus subtilis
physiological function
the PrpD enzyme from Escherichia coli is not a stereospecific 2-methylcitrate dehydratase because it can dehydrate at least two of the four diastereomers of 2-methylcitrate to yield either (E)-2-methylaconitate or (Z)-2-methylaconitate, but the physiological pathways proceed via (Z)-2-methylaconitate, which serves as the substrate for the citB enzyme in the synthesis of 2-methylisocitrate
Escherichia coli
Other publictions for EC 4.2.1.79
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747131
Reddick
First biochemical characteriz ...
Bacillus subtilis 168, Bacillus subtilis, Escherichia coli, Escherichia coli K12
Biochemistry
56
5698-5711
2017
-
-
1
-
-
-
-
-
-
-
-
4
-
8
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
749113
Rocco
The PrpF protein of Shewanell ...
Bacillus subtilis 168, Bacillus subtilis, Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720
PLoS ONE
12
e0188130
2017
-
-
3
-
1
-
-
-
-
-
-
5
-
9
-
-
1
-
-
-
-
-
11
-
3
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
5
-
-
-
1
-
-
-
-
11
-
3
-
-
-
3
-
-
-
-
6
6
-
-
-
728336
Limenitakis
The 2-methylcitrate cycle is i ...
Toxoplasma gondii, Toxoplasma gondii RH
Mol. Microbiol.
87
894-908
2013
-
-
-
-
-
-
-
-
1
-
-
2
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704332
Rocco
In Salmonella enterica, 2-meth ...
Salmonella enterica
J. Bacteriol.
192
771-778
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713880
Peplinski
Investigations on the microbia ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032
Appl. Microbiol. Biotechnol.
88
1145-1159
2010
-
-
-
-
-
-
-
-
-
-
1
-
-
24
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
2
2
1
-
-
706675
Santos
Adaptation to beta-myrcene cat ...
Pseudomonas sp., Pseudomonas sp. M1
Proteomics
9
5101-5111
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681801
Upton
Role of the methylcitrate cycl ...
Mycolicibacterium smegmatis
Microbiology
153
3973-3982
2007
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672679
Mohamed Fahmy Gad El-Rab
Effects of cadmium stress on g ...
Rhodobacter capsulatus, Rhodobacter capsulatus B10
Biosci. Biotechnol. Biochem.
70
2394-2402
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681995
Gould
Dual role of isocitrate lyase ...
Mycobacterium tuberculosis
Mol. Microbiol.
61
940-947
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651735
Grimek
The acnD genes of Shewenella o ...
Shewanella oneidensis, Vibrio cholerae
J. Bacteriol.
186
454-462
2004
4
-
2
-
-
-
-
-
-
2
-
2
-
6
-
-
1
-
-
-
1
-
8
-
1
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
1
-
-
1
-
8
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
488175
Brock
Oxidation of propionate to pyr ...
Escherichia coli
Eur. J. Biochem.
269
6184-6194
2002
1
-
1
-
-
-
-
1
-
-
1
1
-
3
-
-
1
-
-
1
-
-
4
-
-
-
-
-
1
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
1
-
1
-
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
649188
Gulick
Pentaerythritol propoxylate: a ...
Salmonella enterica
Acta Crystallogr. Sect. D
58
306-309
2002
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
653158
Blank
AcnC of Escherichia coli is a ...
Escherichia coli
Microbiology
148
133-146
2002
1
-
1
-
-
-
-
-
-
1
1
1
-
2
-
-
1
-
-
-
2
-
11
1
1
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
2
-
11
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
650059
Horswill
In vitro conversion of propion ...
Salmonella enterica
Biochemistry
40
4703-4713
2001
-
-
1
-
-
-
-
-
-
1
-
2
-
3
-
-
1
-
-
-
3
-
6
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
3
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
5897
Aoki
-
Purification and properties of ...
Yarrowia lipolytica
Agric. Biol. Chem.
45
2831-2837
1981
1
-
-
-
-
-
13
1
1
-
2
1
-
1
-
-
1
-
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
13
-
1
1
-
2
1
-
-
-
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5898
Tabuchi
-
2-Methylcitrate dehydratase, a ...
Yarrowia lipolytica
Agric. Biol. Chem.
45
2823-2929
1981
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
7
-
1
1
2
-
1
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
7
-
1
1
2
-
1
1
1
-
-
-
-
-
-
-
5896
Aoki
-
2-Methylcitrate dehydratase, a ...
Yarrowia lipolytica
Curr. Dev. Yeast Res. , Proc. Int. Yeast Symp. (Stewart, G. G. , Russel, I. , eds. ) Pergamon, Toronto
435-439
1980
-
-
-
-
-
-
-
-
1
-
1
1
-
1
-
-
1
-
-
-
1
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
-
1
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-