BRENDA - Enzyme Database show
show all sequences of 4.2.1.79

AcnC of Escherichia coli is a 2-methylcitrate dehydratase (PrpD) that can use citrate and isocitrate as substrates

Blank, L.; Green, J.; Guest, J.R.; Microbiology 148, 133-146 (2002)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
propionate
induces the enzyme expression from acnC and prpD
Escherichia coli
Cloned(Commentary)
Commentary
Organism
DNA and amino acid sequence determination and analysis, genomic organization, expression of AcnC in AcnABnull strain, overexpression of PrpD in AcnABnull strain
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
enzyme possesses 1 unstable iron-sulfur center per monomer, required for activity, can be reconstituted by Fe2+ under reducing conditions
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
gel filtration and native PAGE
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-Hydroxybutane-1,2,3-tricarboxylate
Escherichia coli
metabolic function
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
Escherichia coli
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
K12, several strains, gene prpD of the propionate catabolism operon prpRBCDE, the enzyme is identical with aconitase AcnC
-
Purification (Commentary)
Commentary
Organism
AcnC partially 0.25fold from AcnABnull strain, PrpD 14.3fold from overexpression in AcnABnull strain
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.005
-
partially purified AcnC
Escherichia coli
0.8
-
purified PrpD
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-malate
-
653158
Escherichia coli
?
-
653158
Escherichia coli
?
2-Hydroxybutane-1,2,3-tricarboxylate
i.e. 2-methylcitrate, best substrate
653158
Escherichia coli
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O
i.e. 2-methyl-cis-aconitate
653158
Escherichia coli
?
2-Hydroxybutane-1,2,3-tricarboxylate
metabolic function
653158
Escherichia coli
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
653158
Escherichia coli
?
citrate
-
653158
Escherichia coli
?
-
653158
Escherichia coli
?
D-malate
-
653158
Escherichia coli
?
-
653158
Escherichia coli
?
D-tartrate
-
653158
Escherichia coli
?
-
653158
Escherichia coli
?
DL-citramalate
-
653158
Escherichia coli
?
-
653158
Escherichia coli
?
DL-isocitrate
-
653158
Escherichia coli
?
-
653158
Escherichia coli
?
L-tartrate
-
653158
Escherichia coli
?
-
653158
Escherichia coli
?
meso-tartrate
-
653158
Escherichia coli
?
-
653158
Escherichia coli
?
additional information
substrate specificity, no activity with cis-aconitate and related substances containing double bonds, overview
653158
Escherichia coli
?
-
653158
Escherichia coli
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 54000, SDS-PAGE
Escherichia coli
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Escherichia coli
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
-
half-life is 30 min for both AcnC and PrpD
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
for AcnC and PrpD
Escherichia coli
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
propionate
induces the enzyme expression from acnC and prpD
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence determination and analysis, genomic organization, expression of AcnC in AcnABnull strain, overexpression of PrpD in AcnABnull strain
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
enzyme possesses 1 unstable iron-sulfur center per monomer, required for activity, can be reconstituted by Fe2+ under reducing conditions
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
gel filtration and native PAGE
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-Hydroxybutane-1,2,3-tricarboxylate
Escherichia coli
metabolic function
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
Escherichia coli
?
Purification (Commentary) (protein specific)
Commentary
Organism
AcnC partially 0.25fold from AcnABnull strain, PrpD 14.3fold from overexpression in AcnABnull strain
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.005
-
partially purified AcnC
Escherichia coli
0.8
-
purified PrpD
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-malate
-
653158
Escherichia coli
?
-
653158
Escherichia coli
?
2-Hydroxybutane-1,2,3-tricarboxylate
i.e. 2-methylcitrate, best substrate
653158
Escherichia coli
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O
i.e. 2-methyl-cis-aconitate
653158
Escherichia coli
?
2-Hydroxybutane-1,2,3-tricarboxylate
metabolic function
653158
Escherichia coli
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
653158
Escherichia coli
?
citrate
-
653158
Escherichia coli
?
-
653158
Escherichia coli
?
D-malate
-
653158
Escherichia coli
?
-
653158
Escherichia coli
?
D-tartrate
-
653158
Escherichia coli
?
-
653158
Escherichia coli
?
DL-citramalate
-
653158
Escherichia coli
?
-
653158
Escherichia coli
?
DL-isocitrate
-
653158
Escherichia coli
?
-
653158
Escherichia coli
?
L-tartrate
-
653158
Escherichia coli
?
-
653158
Escherichia coli
?
meso-tartrate
-
653158
Escherichia coli
?
-
653158
Escherichia coli
?
additional information
substrate specificity, no activity with cis-aconitate and related substances containing double bonds, overview
653158
Escherichia coli
?
-
653158
Escherichia coli
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 54000, SDS-PAGE
Escherichia coli
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Escherichia coli
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
-
half-life is 30 min for both AcnC and PrpD
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
for AcnC and PrpD
Escherichia coli
Other publictions for EC 4.2.1.79
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747131
Reddick
First biochemical characteriz ...
Bacillus subtilis 168, Bacillus subtilis, Escherichia coli, Escherichia coli K12
Biochemistry
56
5698-5711
2017
-
-
1
-
-
-
-
-
-
-
-
4
-
8
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
749113
Rocco
The PrpF protein of Shewanell ...
Bacillus subtilis 168, Bacillus subtilis, Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720
PLoS ONE
12
e0188130
2017
-
-
3
-
1
-
-
-
-
-
-
5
-
9
-
-
1
-
-
-
-
-
11
-
3
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
5
-
-
-
1
-
-
-
-
11
-
3
-
-
-
3
-
-
-
-
6
6
-
-
-
728336
Limenitakis
The 2-methylcitrate cycle is i ...
Toxoplasma gondii, Toxoplasma gondii RH
Mol. Microbiol.
87
894-908
2013
-
-
-
-
-
-
-
-
1
-
-
2
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704332
Rocco
In Salmonella enterica, 2-meth ...
Salmonella enterica
J. Bacteriol.
192
771-778
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713880
Peplinski
Investigations on the microbia ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032
Appl. Microbiol. Biotechnol.
88
1145-1159
2010
-
-
-
-
-
-
-
-
-
-
1
-
-
24
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
2
2
1
-
-
706675
Santos
Adaptation to beta-myrcene cat ...
Pseudomonas sp., Pseudomonas sp. M1
Proteomics
9
5101-5111
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681801
Upton
Role of the methylcitrate cycl ...
Mycolicibacterium smegmatis
Microbiology
153
3973-3982
2007
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672679
Mohamed Fahmy Gad El-Rab
Effects of cadmium stress on g ...
Rhodobacter capsulatus, Rhodobacter capsulatus B10
Biosci. Biotechnol. Biochem.
70
2394-2402
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681995
Gould
Dual role of isocitrate lyase ...
Mycobacterium tuberculosis
Mol. Microbiol.
61
940-947
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651735
Grimek
The acnD genes of Shewenella o ...
Shewanella oneidensis, Vibrio cholerae
J. Bacteriol.
186
454-462
2004
4
-
2
-
-
-
-
-
-
2
-
2
-
6
-
-
1
-
-
-
1
-
8
-
1
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
1
-
-
1
-
8
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
488175
Brock
Oxidation of propionate to pyr ...
Escherichia coli
Eur. J. Biochem.
269
6184-6194
2002
1
-
1
-
-
-
-
1
-
-
1
1
-
3
-
-
1
-
-
1
-
-
4
-
-
-
-
-
1
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
1
-
1
-
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
649188
Gulick
Pentaerythritol propoxylate: a ...
Salmonella enterica
Acta Crystallogr. Sect. D
58
306-309
2002
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
653158
Blank
AcnC of Escherichia coli is a ...
Escherichia coli
Microbiology
148
133-146
2002
1
-
1
-
-
-
-
-
-
1
1
1
-
2
-
-
1
-
-
-
2
-
11
1
1
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
2
-
11
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
650059
Horswill
In vitro conversion of propion ...
Salmonella enterica
Biochemistry
40
4703-4713
2001
-
-
1
-
-
-
-
-
-
1
-
2
-
3
-
-
1
-
-
-
3
-
6
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
3
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
5897
Aoki
-
Purification and properties of ...
Yarrowia lipolytica
Agric. Biol. Chem.
45
2831-2837
1981
1
-
-
-
-
-
13
1
1
-
2
1
-
1
-
-
1
-
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
13
-
1
1
-
2
1
-
-
-
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5898
Tabuchi
-
2-Methylcitrate dehydratase, a ...
Yarrowia lipolytica
Agric. Biol. Chem.
45
2823-2929
1981
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
7
-
1
1
2
-
1
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
7
-
1
1
2
-
1
1
1
-
-
-
-
-
-
-
5896
Aoki
-
2-Methylcitrate dehydratase, a ...
Yarrowia lipolytica
Curr. Dev. Yeast Res. , Proc. Int. Yeast Symp. (Stewart, G. G. , Russel, I. , eds. ) Pergamon, Toronto
435-439
1980
-
-
-
-
-
-
-
-
1
-
1
1
-
1
-
-
1
-
-
-
1
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
-
1
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-