BRENDA - Enzyme Database show
show all sequences of 4.2.1.79

In vitro conversion of propionate to pyruvate by Salmonella enterica enzymes: 2-methylcitrate dehydratase (PrpD) and aconitase Enzymes catalyze the conversion of 2-methylcitrate to 2-methylisocitrate

Horswill, A.R.; Escalante-Semerena, J.C.; Biochemistry 40, 4703-4713 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene prpD in the operon prpBCDE, DNA sequence determination nand analysis, overexpression as N-terminally His-tagged protein in Escherichia coli BL21(DE3)
Salmonella enterica
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no requirement for metal ions and reducing agents
Salmonella enterica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-Hydroxybutane-1,2,3-tricarboxylate
Salmonella enterica
-
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
Salmonella enterica
?
additional information
Salmonella enterica
the enzyme and the aconitase perform the 2-methylcitric acid cycle, overview
?
-
Salmonella enterica
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salmonella enterica
P74840
prpBCDE operon; serovar Typhimurium LT2, gene prpD of the prpBCDE operon
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli BL21(DE3), to homogeneity
Salmonella enterica
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.2
-
purified recombinant His-tagged enzyme, substrate citrate
Salmonella enterica
2.6
-
purified recombinant His-tagged enzyme, substrate cis-aconitate
Salmonella enterica
2.8
-
purified recombinant His-tagged enzyme, substrate 2-methylcitrate
Salmonella enterica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-Hydroxybutane-1,2,3-tricarboxylate
-
650059
Salmonella enterica
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
650059
Salmonella enterica
?
2-Hydroxybutane-1,2,3-tricarboxylate
i.e. 2-methylcitrate
650059
Salmonella enterica
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O
i.e. 2-methyl-cis-aconitate
650059
Salmonella enterica
?
cis-aconitate
-
650059
Salmonella enterica
?
-
650059
Salmonella enterica
?
citrate
low activity with
650059
Salmonella enterica
?
-
650059
Salmonella enterica
?
additional information
enzyme does not catalyze the hydration of 2-methyl-cis-aconitate to 2-methylisocitrate, isocitrate is no substrate
650059
Salmonella enterica
?
-
650059
Salmonella enterica
?
additional information
the enzyme and the aconitase perform the 2-methylcitric acid cycle, overview
650059
Salmonella enterica
?
-
650059
Salmonella enterica
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Salmonella enterica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Salmonella enterica
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
the enzyme contains no iron-sulfur center
Salmonella enterica
Cloned(Commentary) (protein specific)
Commentary
Organism
gene prpD in the operon prpBCDE, DNA sequence determination nand analysis, overexpression as N-terminally His-tagged protein in Escherichia coli BL21(DE3)
Salmonella enterica
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
the enzyme contains no iron-sulfur center
Salmonella enterica
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no requirement for metal ions and reducing agents
Salmonella enterica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-Hydroxybutane-1,2,3-tricarboxylate
Salmonella enterica
-
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
Salmonella enterica
?
additional information
Salmonella enterica
the enzyme and the aconitase perform the 2-methylcitric acid cycle, overview
?
-
Salmonella enterica
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli BL21(DE3), to homogeneity
Salmonella enterica
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.2
-
purified recombinant His-tagged enzyme, substrate citrate
Salmonella enterica
2.6
-
purified recombinant His-tagged enzyme, substrate cis-aconitate
Salmonella enterica
2.8
-
purified recombinant His-tagged enzyme, substrate 2-methylcitrate
Salmonella enterica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-Hydroxybutane-1,2,3-tricarboxylate
-
650059
Salmonella enterica
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
650059
Salmonella enterica
?
2-Hydroxybutane-1,2,3-tricarboxylate
i.e. 2-methylcitrate
650059
Salmonella enterica
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O
i.e. 2-methyl-cis-aconitate
650059
Salmonella enterica
?
cis-aconitate
-
650059
Salmonella enterica
?
-
650059
Salmonella enterica
?
citrate
low activity with
650059
Salmonella enterica
?
-
650059
Salmonella enterica
?
additional information
enzyme does not catalyze the hydration of 2-methyl-cis-aconitate to 2-methylisocitrate, isocitrate is no substrate
650059
Salmonella enterica
?
-
650059
Salmonella enterica
?
additional information
the enzyme and the aconitase perform the 2-methylcitric acid cycle, overview
650059
Salmonella enterica
?
-
650059
Salmonella enterica
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Salmonella enterica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Salmonella enterica
Other publictions for EC 4.2.1.79
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747131
Reddick
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8
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2
2
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749113
Rocco
The PrpF protein of Shewanell ...
Bacillus subtilis 168, Bacillus subtilis, Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720
PLoS ONE
12
e0188130
2017
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3
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1
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5
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9
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1
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5
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1
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11
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3
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3
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6
6
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728336
Limenitakis
The 2-methylcitrate cycle is i ...
Toxoplasma gondii, Toxoplasma gondii RH
Mol. Microbiol.
87
894-908
2013
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1
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2
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704332
Rocco
In Salmonella enterica, 2-meth ...
Salmonella enterica
J. Bacteriol.
192
771-778
2010
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713880
Peplinski
Investigations on the microbia ...
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88
1145-1159
2010
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24
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681801
Upton
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Mycolicibacterium smegmatis
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153
3973-3982
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672679
Mohamed Fahmy Gad El-Rab
Effects of cadmium stress on g ...
Rhodobacter capsulatus, Rhodobacter capsulatus B10
Biosci. Biotechnol. Biochem.
70
2394-2402
2006
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681995
Gould
Dual role of isocitrate lyase ...
Mycobacterium tuberculosis
Mol. Microbiol.
61
940-947
2006
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651735
Grimek
The acnD genes of Shewenella o ...
Shewanella oneidensis, Vibrio cholerae
J. Bacteriol.
186
454-462
2004
4
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2
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488175
Brock
Oxidation of propionate to pyr ...
Escherichia coli
Eur. J. Biochem.
269
6184-6194
2002
1
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649188
Gulick
Pentaerythritol propoxylate: a ...
Salmonella enterica
Acta Crystallogr. Sect. D
58
306-309
2002
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653158
Blank
AcnC of Escherichia coli is a ...
Escherichia coli
Microbiology
148
133-146
2002
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650059
Horswill
In vitro conversion of propion ...
Salmonella enterica
Biochemistry
40
4703-4713
2001
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5897
Aoki
-
Purification and properties of ...
Yarrowia lipolytica
Agric. Biol. Chem.
45
2831-2837
1981
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5898
Tabuchi
-
2-Methylcitrate dehydratase, a ...
Yarrowia lipolytica
Agric. Biol. Chem.
45
2823-2929
1981
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5896
Aoki
-
2-Methylcitrate dehydratase, a ...
Yarrowia lipolytica
Curr. Dev. Yeast Res. , Proc. Int. Yeast Symp. (Stewart, G. G. , Russel, I. , eds. ) Pergamon, Toronto
435-439
1980
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