In vitro conversion of propionate to pyruvate by Salmonella enterica enzymes: 2-methylcitrate dehydratase (PrpD) and aconitase Enzymes catalyze the conversion of 2-methylcitrate to 2-methylisocitrate

Horswill, A.R.; Escalante-Semerena, J.C.; Biochemistry 40, 4703-4713 (2001)

Data extracted from this reference:

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

2-Hydroxybutane-1,2,3-tricarboxylate

Salmonella enterica

-

(Z)-But-2-ene-1,2,3-tricarboxylate + H2O

-

Salmonella enterica

?

additional information

Salmonella enterica

the enzyme and the aconitase perform the 2-methylcitric acid cycle, overview

?

-

Salmonella enterica

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

2-Hydroxybutane-1,2,3-tricarboxylate

-

650059

Salmonella enterica

(Z)-But-2-ene-1,2,3-tricarboxylate + H2O

-

650059

Salmonella enterica

?

2-Hydroxybutane-1,2,3-tricarboxylate

i.e. 2-methylcitrate

650059

Salmonella enterica

(Z)-But-2-ene-1,2,3-tricarboxylate + H2O

i.e. 2-methyl-cis-aconitate

650059

Salmonella enterica

?

cis-aconitate

-

650059

Salmonella enterica

?

-

650059

Salmonella enterica

?

citrate

low activity with

650059

Salmonella enterica

?

-

650059

Salmonella enterica

?

additional information

enzyme does not catalyze the hydration of 2-methyl-cis-aconitate to 2-methylisocitrate, isocitrate is no substrate

650059

Salmonella enterica

?

-

650059

Salmonella enterica

?

additional information

the enzyme and the aconitase perform the 2-methylcitric acid cycle, overview

650059

Salmonella enterica

?

-

650059

Salmonella enterica

?

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

2-Hydroxybutane-1,2,3-tricarboxylate

Salmonella enterica

-

(Z)-But-2-ene-1,2,3-tricarboxylate + H2O

-

Salmonella enterica

?

additional information

Salmonella enterica

the enzyme and the aconitase perform the 2-methylcitric acid cycle, overview

?

-

Salmonella enterica

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

2-Hydroxybutane-1,2,3-tricarboxylate

-

650059

Salmonella enterica

(Z)-But-2-ene-1,2,3-tricarboxylate + H2O

-

650059

Salmonella enterica

?

2-Hydroxybutane-1,2,3-tricarboxylate

i.e. 2-methylcitrate

650059

Salmonella enterica

(Z)-But-2-ene-1,2,3-tricarboxylate + H2O

i.e. 2-methyl-cis-aconitate

650059

Salmonella enterica

?

cis-aconitate

-

650059

Salmonella enterica

?

-

650059

Salmonella enterica

?

citrate

low activity with

650059

Salmonella enterica

?

-

650059

Salmonella enterica

?

additional information

enzyme does not catalyze the hydration of 2-methyl-cis-aconitate to 2-methylisocitrate, isocitrate is no substrate

650059

Salmonella enterica

?

-

650059

Salmonella enterica

?

additional information

the enzyme and the aconitase perform the 2-methylcitric acid cycle, overview

650059

Salmonella enterica

?

-

650059

Salmonella enterica

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

747131

Reddick

First biochemical characteriz ...

Bacillus subtilis 168, Bacillus subtilis, Escherichia coli, Escherichia coli K12

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5698-5711

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2

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749113

Rocco

The PrpF protein of Shewanell ...

Bacillus subtilis 168, Bacillus subtilis, Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720

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The 2-methylcitrate cycle is i ...

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Mycolicibacterium smegmatis

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Dual role of isocitrate lyase ...

Mycobacterium tuberculosis

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Brock

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Blank

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650059

Horswill

In vitro conversion of propion ...

Salmonella enterica

Biochemistry

40

4703-4713

2001

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1

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Aoki

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1

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5896

Aoki

-

2-Methylcitrate dehydratase, a ...

Yarrowia lipolytica

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