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Literature summary for 4.2.1.79 extracted from

  • Aoki, H.; Tabuchi, T.
    Purification and properties of 2-methylcitrate dehydratase from Yarrowia lipolytica (1981), Agric. Biol. Chem., 45, 2831-2837.
No PubMed abstract available
Search for more literature for 4.2.1.79

Activating Compound

Activating Compound Comment Organism Structure
additional information no requirement for AMP, ADT, ATP, NAD(P)+, NAD(P)H, pyruvate Yarrowia lipolytica

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Yarrowia lipolytica
citrate competitive Yarrowia lipolytica
Cu2+
-
 Yarrowia lipolytica
DL-Fluorocitrate competitive Yarrowia lipolytica
Hg2+
-
 Yarrowia lipolytica
iodoacetate
-
 Yarrowia lipolytica
additional information not: chelating agents Yarrowia lipolytica
Ni2+
-
 Yarrowia lipolytica
p-chloromercuribenzoate
-
 Yarrowia lipolytica
Pb2+
-
 Yarrowia lipolytica
threo-Isocitrate competitive Yarrowia lipolytica
tricarballylate competitive Yarrowia lipolytica
Zn2+
-
 Yarrowia lipolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 2-Hydroxybutane-1,2,3-tricarboxylate diastereomer Yarrowia lipolytica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Yarrowia lipolytica 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000 79000 gel filtration Yarrowia lipolytica
79000
-
 1 * 79000, SDS-PAGE Yarrowia lipolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-Hydroxybutane-1,2,3-tricarboxylate Yarrowia lipolytica methylcitric acid cycle, propionyl-CoA oxidation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Yarrowia lipolytica
-
 formerly Saccaromycopsis lipolytica
-

Purification (Commentary)

Purification (Comment) Organism
-
 Yarrowia lipolytica

Storage Stability

Storage Stability Organism
liquid N2, 0.05 M potassium phosphate buffer, pH 6.5, stable for more than 6 months Yarrowia lipolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Hydroxybutane-1,2,3-tricarboxylate i.e. 2-methylcitrate Yarrowia lipolytica (Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
 ?
2-Hydroxybutane-1,2,3-tricarboxylate methylcitric acid cycle, propionyl-CoA oxidation Yarrowia lipolytica ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 79000, SDS-PAGE Yarrowia lipolytica