Application | Comment | Organism |
---|---|---|
synthesis | the enzyme can serve as a tool to synthesize unnatural, optically active tetrahydroisoquinolines. The enzyme is a promising catalyst that functions to stereoselectively produce various 1-substituted-1,2,3,4-tetrahydroisoquinolines | Coptis japonica |
Cloned (Comment) | Organism |
---|---|
N-terminally truncated enzyme (CjNCS-DELTA29, with a deletion of the first 29 amino acid residues) is expressed in Escherichia coli. The cell-free extract of Escherichia coli expressing CjNCS-DELTA29 exhibits 40fold higher activity than that expressing intact enzyme | Coptis japonica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylacetaldehyde + dopamine | Coptis japonica | the enzyme catalyzes the stereoselective Pictet-Spengler reaction between dopamine and 4-hydroxyphenylacetaldehyde as a step of benzylisoquinoline alkaloid synthesis in plants | (S)-norcoclaurine + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coptis japonica | A2A1A1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butylaldehyde + dopamine | molar yield and enantiomeric excess of 6,7-dihydroxy-1-phenethyl-1,2,3,4-tetrahydroisoquinoline are 99.6 and 98.0 %, respectively | Coptis japonica | 6,7-dihydroxy-1-propyl-1,2,3,4-tetrahydroisoquinoline | - |
? | |
4-hydroxyphenylacetaldehyde + dopamine | - |
Coptis japonica | (S)-norcoclaurine + H2O | - |
? | |
4-hydroxyphenylacetaldehyde + dopamine | the enzyme catalyzes the stereoselective Pictet-Spengler reaction between dopamine and 4-hydroxyphenylacetaldehyde as a step of benzylisoquinoline alkaloid synthesis in plants | Coptis japonica | (S)-norcoclaurine + H2O | - |
? | |
hydrocinnamaldehyde + dopamine | molar yield and enantiomeric excess of 6,7-dihydroxy-1-phenethyl-1,2,3,4-tetrahydroisoquinoline are 86.0 and 95.3%, respectively | Coptis japonica | 6,7-dihydroxy-1-phenethyl-1,2,3,4-tetrahydroisoquinoline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CjPR10A | - |
Coptis japonica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Coptis japonica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Coptis japonica |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the stereoselective Pictet-Spengler reaction between dopamine and 4-hydroxyphenylacetaldehyde as a step of benzylisoquinoline alkaloid synthesis in plants | Coptis japonica |