Cloned (Comment) | Organism |
---|---|
unlabelled and 15N-labelled delta 29NCS is expressed using the expression system Escherichia coli Rosetta(DE3)/pET29b-delta 29NCS | Thalictrum flavum |
Crystallization (Comment) | Organism |
---|---|
combining experimental NMR spectroscopic data with homology modelling using the homologous major birch pollen allergen Bet v 1 (PDB code 1BV1) as template structure a reliable model structure of delta 29NCS is built | Thalictrum flavum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde | Thalictrum flavum | - |
(S)-Norcoclaurine + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thalictrum flavum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thalictrum flavum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde | - |
Thalictrum flavum | (S)-Norcoclaurine + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | size-exclusion chromatography (SEC) and NMR relaxation measurements, concentration-dependent oligomerization of delta 29NCS with an equilibrium of monomeric and oligomeric protein over a broad concentration range, at low protein concentrations in the range of 10 microM the enzyme is predominantly monomeric, no indication that substrate binding induces dimerization of delta 29NCS | Thalictrum flavum |
Synonyms | Comment | Organism |
---|---|---|
(S)-norcoclaurine synthase | - |
Thalictrum flavum |
NCS | - |
Thalictrum flavum |