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Literature summary for 4.2.1.77 extracted from

  • Watanabe, S.; Fukumori, F.; Miyazaki, M.; Tagami, S.; Watanabe, Y.
    Characterization of a novel cis-3-hydroxy-L-proline dehydratase and a trans-3-hydroxy-L-proline dehydratase from Bacteria (2017), J. Bacteriol., 199, e00255-17 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Azospirillum brasilense
expression in Escherichia coli BL21(DE3) Micromonospora viridifaciens

Protein Variants

Protein Variants Comment Organism
C261T the kcat/Km value is 826fold lower than that of the wild-type enzyme, and this is mainly attributed to a 162fold increase in Km Azospirillum brasilense
C261T the kcat/Km value is 826fold lower than that of the wild-type enzyme, and this is mainly attributed to a 162fold increase in Km Micromonospora viridifaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00291
-
trans-3-hydroxy-L-proline pH 8.0, 30°C, wild-type enzyme Micromonospora viridifaciens
0.0472
-
trans-3-hydroxy-L-proline pH 8.0, 30°C, mutant enzyme C261T Micromonospora viridifaciens
2.79
-
trans-3-hydroxy-L-proline pH 8.0, 30°C, wild-type enzyme Colwellia psychrerythraea
14.3
-
trans-3-hydroxy-L-proline pH 8.0, 30°C, wild-type enzyme Azospirillum brasilense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trans-3-hydroxy-L-proline Azospirillum brasilense
-
1-pyrroline 2-carboxylate + H2O
-
?
trans-3-hydroxy-L-proline Colwellia psychrerythraea
-
1-pyrroline 2-carboxylate + H2O
-
?
trans-3-hydroxy-L-proline Micromonospora viridifaciens
-
1-pyrroline 2-carboxylate + H2O
-
?
trans-3-hydroxy-L-proline Azospirillum brasilense NBRC 102289
-
1-pyrroline 2-carboxylate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Azospirillum brasilense
-
-
-
Azospirillum brasilense NBRC 102289
-
-
-
Colwellia psychrerythraea Q485S0
-
-
Micromonospora viridifaciens A0A1C4WR37 cf. EC 5.1.1.4
-

Purification (Commentary)

Purification (Comment) Organism
-
Azospirillum brasilense
-
Micromonospora viridifaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.877
-
substrate: trans-3-hydroxy-L-proline, pH 8.0, 30°C, mutant enzyme C261T Micromonospora viridifaciens
17.6
-
substrate: trans-3-hydroxy-L-proline, pH 8.0, 30°C, wild-type enzyme Micromonospora viridifaciens
25.4
-
substrate: trans-3-hydroxy-L-proline, pH 8.0, 30°C, wild-type enzyme Colwellia psychrerythraea
26.1
-
substrate: trans-3-hydroxy-L-proline, pH 8.0, 30°C, wild-type enzyme Azospirillum brasilense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trans-3-hydroxy-L-proline
-
Azospirillum brasilense 1-pyrroline 2-carboxylate + H2O
-
?
trans-3-hydroxy-L-proline
-
Colwellia psychrerythraea 1-pyrroline 2-carboxylate + H2O
-
?
trans-3-hydroxy-L-proline
-
Micromonospora viridifaciens 1-pyrroline 2-carboxylate + H2O
-
?
trans-3-hydroxy-L-proline
-
Azospirillum brasilense NBRC 102289 1-pyrroline 2-carboxylate + H2O
-
?

Synonyms

Synonyms Comment Organism
AbHypG
-
Azospirillum brasilense
bifunctional T3LHyp dehydratase/2-epimerase
-
Micromonospora viridifaciens
CpHypG
-
Colwellia psychrerythraea
MvHypA2
-
Micromonospora viridifaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.43
-
trans-3-hydroxy-L-proline pH 8.0, 30°C, mutant enzyme C261T Micromonospora viridifaciens
3.4
-
trans-3-hydroxy-L-proline pH 8.0, 30°C, wild-type enzyme Micromonospora viridifaciens
22.17
-
trans-3-hydroxy-L-proline pH 8.0, 30°C, wild-type enzyme Colwellia psychrerythraea
37.33
-
trans-3-hydroxy-L-proline pH 8.0, 30°C, wild-type enzyme Azospirillum brasilense

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.052
-
trans-3-hydroxy-L-proline pH 8.0, 30°C, mutant enzyme C261T Micromonospora viridifaciens
2.62
-
trans-3-hydroxy-L-proline pH 8.0, 30°C, wild-type enzyme Azospirillum brasilense
7.95
-
trans-3-hydroxy-L-proline pH 8.0, 30°C, wild-type enzyme Colwellia psychrerythraea
42.7
-
trans-3-hydroxy-L-proline pH 8.0, 30°C, wild-type enzyme Micromonospora viridifaciens