Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Azospirillum brasilense |
expression in Escherichia coli BL21(DE3) | Micromonospora viridifaciens |
Protein Variants | Comment | Organism |
---|---|---|
C261T | the kcat/Km value is 826fold lower than that of the wild-type enzyme, and this is mainly attributed to a 162fold increase in Km | Azospirillum brasilense |
C261T | the kcat/Km value is 826fold lower than that of the wild-type enzyme, and this is mainly attributed to a 162fold increase in Km | Micromonospora viridifaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00291 | - |
trans-3-hydroxy-L-proline | pH 8.0, 30°C, wild-type enzyme | Micromonospora viridifaciens | |
0.0472 | - |
trans-3-hydroxy-L-proline | pH 8.0, 30°C, mutant enzyme C261T | Micromonospora viridifaciens | |
2.79 | - |
trans-3-hydroxy-L-proline | pH 8.0, 30°C, wild-type enzyme | Colwellia psychrerythraea | |
14.3 | - |
trans-3-hydroxy-L-proline | pH 8.0, 30°C, wild-type enzyme | Azospirillum brasilense |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-3-hydroxy-L-proline | Azospirillum brasilense | - |
1-pyrroline 2-carboxylate + H2O | - |
? | |
trans-3-hydroxy-L-proline | Colwellia psychrerythraea | - |
1-pyrroline 2-carboxylate + H2O | - |
? | |
trans-3-hydroxy-L-proline | Micromonospora viridifaciens | - |
1-pyrroline 2-carboxylate + H2O | - |
? | |
trans-3-hydroxy-L-proline | Azospirillum brasilense NBRC 102289 | - |
1-pyrroline 2-carboxylate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azospirillum brasilense | - |
- |
- |
Azospirillum brasilense NBRC 102289 | - |
- |
- |
Colwellia psychrerythraea | Q485S0 | - |
- |
Micromonospora viridifaciens | A0A1C4WR37 | cf. EC 5.1.1.4 | - |
Purification (Comment) | Organism |
---|---|
- |
Azospirillum brasilense |
- |
Micromonospora viridifaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.877 | - |
substrate: trans-3-hydroxy-L-proline, pH 8.0, 30°C, mutant enzyme C261T | Micromonospora viridifaciens |
17.6 | - |
substrate: trans-3-hydroxy-L-proline, pH 8.0, 30°C, wild-type enzyme | Micromonospora viridifaciens |
25.4 | - |
substrate: trans-3-hydroxy-L-proline, pH 8.0, 30°C, wild-type enzyme | Colwellia psychrerythraea |
26.1 | - |
substrate: trans-3-hydroxy-L-proline, pH 8.0, 30°C, wild-type enzyme | Azospirillum brasilense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-3-hydroxy-L-proline | - |
Azospirillum brasilense | 1-pyrroline 2-carboxylate + H2O | - |
? | |
trans-3-hydroxy-L-proline | - |
Colwellia psychrerythraea | 1-pyrroline 2-carboxylate + H2O | - |
? | |
trans-3-hydroxy-L-proline | - |
Micromonospora viridifaciens | 1-pyrroline 2-carboxylate + H2O | - |
? | |
trans-3-hydroxy-L-proline | - |
Azospirillum brasilense NBRC 102289 | 1-pyrroline 2-carboxylate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AbHypG | - |
Azospirillum brasilense |
bifunctional T3LHyp dehydratase/2-epimerase | - |
Micromonospora viridifaciens |
CpHypG | - |
Colwellia psychrerythraea |
MvHypA2 | - |
Micromonospora viridifaciens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.43 | - |
trans-3-hydroxy-L-proline | pH 8.0, 30°C, mutant enzyme C261T | Micromonospora viridifaciens | |
3.4 | - |
trans-3-hydroxy-L-proline | pH 8.0, 30°C, wild-type enzyme | Micromonospora viridifaciens | |
22.17 | - |
trans-3-hydroxy-L-proline | pH 8.0, 30°C, wild-type enzyme | Colwellia psychrerythraea | |
37.33 | - |
trans-3-hydroxy-L-proline | pH 8.0, 30°C, wild-type enzyme | Azospirillum brasilense |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.052 | - |
trans-3-hydroxy-L-proline | pH 8.0, 30°C, mutant enzyme C261T | Micromonospora viridifaciens | |
2.62 | - |
trans-3-hydroxy-L-proline | pH 8.0, 30°C, wild-type enzyme | Azospirillum brasilense | |
7.95 | - |
trans-3-hydroxy-L-proline | pH 8.0, 30°C, wild-type enzyme | Colwellia psychrerythraea | |
42.7 | - |
trans-3-hydroxy-L-proline | pH 8.0, 30°C, wild-type enzyme | Micromonospora viridifaciens |