BRENDA - Enzyme Database show
show all sequences of 4.2.1.76

Biosynthesis of UDP-4-keto-6-deoxyglucose and UDP-rhamnose in pathogenic fungi Magnaporthe grisea and Botryotinia fuckeliana

Martinez, V.; Ingwers, M.; Smith, J.; Glushka, J.; Yang, T.; Bar-Peled, M.; J. Biol. Chem. 287, 879-892 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21 cells
Botrytis cinerea
expressed in Escherichia coli BL21 cells
Magnaporthe grisea
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
not inhibited by NADH
Botrytis cinerea
additional information
not inhibited by NADH
Magnaporthe grisea
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
3 * 52000, SDS-PAGE
Magnaporthe grisea
52500
-
3 * 52500, SDS-PAGE
Botrytis cinerea
140000
-
gel filtration
Botrytis cinerea
140000
-
gel filtration
Magnaporthe grisea
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-glucose
Botrytis cinerea
-
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
?
UDP-glucose
Magnaporthe grisea
-
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
?
UDP-glucose
Magnaporthe grisea CP987
-
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
?
UDP-glucose
Botrytis cinerea B05.10
-
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Botrytis cinerea
F8U971
-
-
Botrytis cinerea B05.10
F8U971
-
-
Magnaporthe grisea
F8U969
-
-
Magnaporthe grisea CP987
F8U969
-
-
Purification (Commentary)
Commentary
Organism
nickel-Sepharose column chromatography
Botrytis cinerea
nickel-Sepharose column chromatography
Magnaporthe grisea
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTDP-glucose
this enzymatic reaction is much less efficient compared to UDP-glucose
730005
Botrytis cinerea
dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
dTDP-glucose
this enzymatic reaction is much less efficient compared to UDP-glucose
730005
Magnaporthe grisea
dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
dTDP-glucose
this enzymatic reaction is much less efficient compared to UDP-glucose
730005
Magnaporthe grisea CP987
dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
dTDP-glucose
this enzymatic reaction is much less efficient compared to UDP-glucose
730005
Botrytis cinerea B05.10
dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
UDP-glucose
-
730005
Botrytis cinerea
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
UDP-glucose
-
730005
Magnaporthe grisea
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
UDP-glucose
-
730005
Magnaporthe grisea CP987
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
UDP-glucose
-
730005
Botrytis cinerea B05.10
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotrimer
3 * 52500, SDS-PAGE
Botrytis cinerea
homotrimer
3 * 52000, SDS-PAGE
Magnaporthe grisea
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
30
-
Botrytis cinerea
25
30
-
Magnaporthe grisea
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Botrytis cinerea
7
-
-
Magnaporthe grisea
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
required
Botrytis cinerea
NAD+
required
Magnaporthe grisea
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21 cells
Botrytis cinerea
expressed in Escherichia coli BL21 cells
Magnaporthe grisea
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
required
Botrytis cinerea
NAD+
required
Magnaporthe grisea
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
not inhibited by NADH
Botrytis cinerea
additional information
not inhibited by NADH
Magnaporthe grisea
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
3 * 52000, SDS-PAGE
Magnaporthe grisea
52500
-
3 * 52500, SDS-PAGE
Botrytis cinerea
140000
-
gel filtration
Botrytis cinerea
140000
-
gel filtration
Magnaporthe grisea
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-glucose
Botrytis cinerea
-
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
?
UDP-glucose
Magnaporthe grisea
-
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
?
UDP-glucose
Magnaporthe grisea CP987
-
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
?
UDP-glucose
Botrytis cinerea B05.10
-
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
nickel-Sepharose column chromatography
Botrytis cinerea
nickel-Sepharose column chromatography
Magnaporthe grisea
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTDP-glucose
this enzymatic reaction is much less efficient compared to UDP-glucose
730005
Botrytis cinerea
dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
dTDP-glucose
this enzymatic reaction is much less efficient compared to UDP-glucose
730005
Magnaporthe grisea
dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
dTDP-glucose
this enzymatic reaction is much less efficient compared to UDP-glucose
730005
Magnaporthe grisea CP987
dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
dTDP-glucose
this enzymatic reaction is much less efficient compared to UDP-glucose
730005
Botrytis cinerea B05.10
dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
UDP-glucose
-
730005
Botrytis cinerea
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
UDP-glucose
-
730005
Magnaporthe grisea
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
UDP-glucose
-
730005
Magnaporthe grisea CP987
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
UDP-glucose
-
730005
Botrytis cinerea B05.10
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotrimer
3 * 52500, SDS-PAGE
Botrytis cinerea
homotrimer
3 * 52000, SDS-PAGE
Magnaporthe grisea
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
30
-
Botrytis cinerea
25
30
-
Magnaporthe grisea
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Botrytis cinerea
7
-
-
Magnaporthe grisea
Other publictions for EC 4.2.1.76
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730005
Martinez
Biosynthesis of UDP-4-keto-6-d ...
Botrytis cinerea, Botrytis cinerea B05.10, Magnaporthe grisea, Magnaporthe grisea CP987
J. Biol. Chem.
287
879-892
2012
-
-
2
-
-
-
2
-
-
-
4
4
-
12
-
-
2
-
-
-
-
-
8
2
2
-
-
-
2
-
-
2
-
-
-
-
-
2
2
-
-
-
-
2
-
-
-
-
4
4
-
-
-
2
-
-
-
-
8
2
2
-
-
-
2
-
-
-
-
-
-
-
-
-
730794
Sen
UDP-glucose 4, 6-dehydratase a ...
Candida albicans
PLoS Pathog.
7
e1002384
2011
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
716065
Parakkottil Chothi
Identification of an L-rhamnos ...
Acanthamoeba polyphaga Mimivirus, Acanthocystis turfacea chlorella virus 1
J. Virol.
84
8829-8838
2010
1
-
2
-
-
-
2
1
-
-
6
4
-
10
-
-
1
-
-
-
2
-
8
3
2
-
2
-
2
-
-
1
-
-
-
1
-
2
1
-
-
-
-
2
-
1
-
-
6
4
-
-
-
1
-
-
2
-
8
3
2
-
2
-
2
-
-
-
-
2
2
-
-
-
680873
Oka
Functional analysis of Arabido ...
Arabidopsis thaliana
J. Biol. Chem.
282
5389-5403
2007
-
-
1
-
5
-
4
1
-
-
1
-
-
4
-
-
-
-
-
-
-
-
1
-
1
1
-
1
1
1
-
-
-
-
-
-
-
1
-
-
5
-
-
4
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
1
-
1
1
1
-
-
-
-
-
-
-
-
5892
Kamsteeg
The formation of UDP-L-rhamnos ...
Silene dioica
FEBS Lett.
91
281-284
1978
-
-
-
-
-
-
1
1
-
-
-
1
-
1
-
-
1
-
-
2
-
-
2
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
1
-
2
-
-
2
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-