BRENDA - Enzyme Database show
show all sequences of 4.2.1.76

Identification of an L-rhamnose synthetic pathway in two nucleocytoplasmic large DNA viruses

Parakkottil Chothi, M.; Duncan, G.; Armirotti, A.; Abergel, C.; Gurnon, J.; Van Etten, J.; Bernardi, C.; Damonte, G.; Tonetti, M.; J. Virol. 84, 8829-8838 (2010)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
NAD+
mimivirus UGD requires exogenous NAD+ for activity during purification
Acanthamoeba polyphaga Mimivirus
Cloned(Commentary)
Commentary
Organism
gene R141, transcription profiling of UGD, phylogenetic analysis
Acanthamoeba polyphaga Mimivirus
gene Z544R, UGD transcription profiling, phylogenetic analysis, expression of the two GST-tagged UGD isozymes in Escherichia coli
Acanthocystis turfacea chlorella virus 1
Inhibitors
Inhibitors
Commentary
Organism
Structure
UDP-L-rhamnose
feedback inhibition of UGD, 60% inhibition at 0.1 mM
Acanthamoeba polyphaga Mimivirus
UDP-L-rhamnose
feedback inhibition of UGD, 50% inhibition at 0.1 mM
Acanthocystis turfacea chlorella virus 1
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0184
-
UDP-D-glucose
purified ATCV-1 UGD, pH 7.5, 25°C
Acanthocystis turfacea chlorella virus 1
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
x * 33000, UGER, SDS-PAGE
Acanthamoeba polyphaga Mimivirus
37000
-
2 * 37000, UGD, SDS-PAGE
Acanthamoeba polyphaga Mimivirus
39000
-
2 * 39000, UGD, SDS-PAGE
Acanthocystis turfacea chlorella virus 1
47000
-
UGER, gel filtration
Acanthamoeba polyphaga Mimivirus
75000
-
UGD, gel filtration
Acanthocystis turfacea chlorella virus 1
100000
-
UGD, gel filtration
Acanthamoeba polyphaga Mimivirus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dTDP-D-glucose
Acanthamoeba polyphaga Mimivirus
-
dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
?
dTDP-D-glucose
Acanthocystis turfacea chlorella virus 1
-
dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
UDP-D-glucose
Acanthocystis turfacea chlorella virus 1
-
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
?
UDP-D-glucose
Acanthamoeba polyphaga Mimivirus
UGD proteins prefer UDP-D-glucose as a substrate
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acanthamoeba polyphaga Mimivirus
-
gene R141
-
Acanthocystis turfacea chlorella virus 1
-
ATCV-1, two UGD isozymes, gene Z544R, propagated in Chlorella strain SAG 3.83
-
Purification (Commentary)
Commentary
Organism
recombinant GST-tagged isozymes from Escherichia coli by glutathione affinity chromatography
Acanthocystis turfacea chlorella virus 1
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0042
-
purified UGD, pH 7.5-8.5, 25°C, 0.1 mM NAD+
Acanthamoeba polyphaga Mimivirus
0.023
-
purified ATCV-1 UGD, pH 7.5, 25°C
Acanthocystis turfacea chlorella virus 1
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTDP-D-glucose
-
716065
Acanthamoeba polyphaga Mimivirus
dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
dTDP-D-glucose
-
716065
Acanthocystis turfacea chlorella virus 1
dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
-
dTDP-D-glucose
UGD activity with dTDP-D-glucose is less than 10% of that with UDPD-glucose
716065
Acanthocystis turfacea chlorella virus 1
dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
additional information
product identification by electrospray ionization-mass spectrometry and gas chromatography mass spectrometry
716065
Acanthamoeba polyphaga Mimivirus
?
-
-
-
-
additional information
product identification by electrospray ionization-mass spectrometry and gas chromatography mass spectrometry
716065
Acanthocystis turfacea chlorella virus 1
?
-
-
-
-
UDP-D-glucose
-
716065
Acanthocystis turfacea chlorella virus 1
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
UDP-D-glucose
UGD proteins prefer UDP-D-glucose as a substrate
716065
Acanthamoeba polyphaga Mimivirus
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
UDP-D-glucose
UGD proteins prefer UDP-D-glucose as a substrate
716065
Acanthocystis turfacea chlorella virus 1
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 37000, UGD, SDS-PAGE
Acanthamoeba polyphaga Mimivirus
dimer
2 * 39000, UGD, SDS-PAGE
Acanthocystis turfacea chlorella virus 1
monomer or dimer
x * 33000, UGER, SDS-PAGE
Acanthamoeba polyphaga Mimivirus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Acanthamoeba polyphaga Mimivirus
25
-
assay at
Acanthocystis turfacea chlorella virus 1
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
20
30
purified UGD, activity decreases above 20°C, complete inactivation after 30 min
Acanthamoeba polyphaga Mimivirus
20
30
purified UGD, activity decreases above 20°C, complete inactivation after 30 min
Acanthocystis turfacea chlorella virus 1
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
UGD
Acanthamoeba polyphaga Mimivirus
7.5
-
-
Acanthocystis turfacea chlorella virus 1
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
tightly bound cofactor
Acanthocystis turfacea chlorella virus 1
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
NAD+
mimivirus UGD requires exogenous NAD+ for activity during purification
Acanthamoeba polyphaga Mimivirus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene R141, transcription profiling of UGD, phylogenetic analysis
Acanthamoeba polyphaga Mimivirus
gene Z544R, UGD transcription profiling, phylogenetic analysis, expression of the two GST-tagged UGD isozymes in Escherichia coli
Acanthocystis turfacea chlorella virus 1
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
tightly bound cofactor
Acanthocystis turfacea chlorella virus 1
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
UDP-L-rhamnose
feedback inhibition of UGD, 60% inhibition at 0.1 mM
Acanthamoeba polyphaga Mimivirus
UDP-L-rhamnose
feedback inhibition of UGD, 50% inhibition at 0.1 mM
Acanthocystis turfacea chlorella virus 1
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0184
-
UDP-D-glucose
purified ATCV-1 UGD, pH 7.5, 25°C
Acanthocystis turfacea chlorella virus 1
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
x * 33000, UGER, SDS-PAGE
Acanthamoeba polyphaga Mimivirus
37000
-
2 * 37000, UGD, SDS-PAGE
Acanthamoeba polyphaga Mimivirus
39000
-
2 * 39000, UGD, SDS-PAGE
Acanthocystis turfacea chlorella virus 1
47000
-
UGER, gel filtration
Acanthamoeba polyphaga Mimivirus
75000
-
UGD, gel filtration
Acanthocystis turfacea chlorella virus 1
100000
-
UGD, gel filtration
Acanthamoeba polyphaga Mimivirus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dTDP-D-glucose
Acanthamoeba polyphaga Mimivirus
-
dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
?
dTDP-D-glucose
Acanthocystis turfacea chlorella virus 1
-
dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
UDP-D-glucose
Acanthocystis turfacea chlorella virus 1
-
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
?
UDP-D-glucose
Acanthamoeba polyphaga Mimivirus
UGD proteins prefer UDP-D-glucose as a substrate
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant GST-tagged isozymes from Escherichia coli by glutathione affinity chromatography
Acanthocystis turfacea chlorella virus 1
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0042
-
purified UGD, pH 7.5-8.5, 25°C, 0.1 mM NAD+
Acanthamoeba polyphaga Mimivirus
0.023
-
purified ATCV-1 UGD, pH 7.5, 25°C
Acanthocystis turfacea chlorella virus 1
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTDP-D-glucose
-
716065
Acanthamoeba polyphaga Mimivirus
dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
dTDP-D-glucose
-
716065
Acanthocystis turfacea chlorella virus 1
dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
-
dTDP-D-glucose
UGD activity with dTDP-D-glucose is less than 10% of that with UDPD-glucose
716065
Acanthocystis turfacea chlorella virus 1
dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
additional information
product identification by electrospray ionization-mass spectrometry and gas chromatography mass spectrometry
716065
Acanthamoeba polyphaga Mimivirus
?
-
-
-
-
additional information
product identification by electrospray ionization-mass spectrometry and gas chromatography mass spectrometry
716065
Acanthocystis turfacea chlorella virus 1
?
-
-
-
-
UDP-D-glucose
-
716065
Acanthocystis turfacea chlorella virus 1
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
UDP-D-glucose
UGD proteins prefer UDP-D-glucose as a substrate
716065
Acanthamoeba polyphaga Mimivirus
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
UDP-D-glucose
UGD proteins prefer UDP-D-glucose as a substrate
716065
Acanthocystis turfacea chlorella virus 1
UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 37000, UGD, SDS-PAGE
Acanthamoeba polyphaga Mimivirus
dimer
2 * 39000, UGD, SDS-PAGE
Acanthocystis turfacea chlorella virus 1
monomer or dimer
x * 33000, UGER, SDS-PAGE
Acanthamoeba polyphaga Mimivirus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Acanthamoeba polyphaga Mimivirus
25
-
assay at
Acanthocystis turfacea chlorella virus 1
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
20
30
purified UGD, activity decreases above 20°C, complete inactivation after 30 min
Acanthamoeba polyphaga Mimivirus
20
30
purified UGD, activity decreases above 20°C, complete inactivation after 30 min
Acanthocystis turfacea chlorella virus 1
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
UGD
Acanthamoeba polyphaga Mimivirus
7.5
-
-
Acanthocystis turfacea chlorella virus 1
General Information
General Information
Commentary
Organism
evolution
phylogenetic analysis indicates that ATCV-1 has probably acquired its UGD gene via a recent horizontal gene transfer from a green algal host, while an earlier event involving the complete pathway probably occurred between a protozoan ancestor and mimivirus. Chloroviruses, and maybe mimivirus, may encode most, if not all, of the glycosylation machinery involved in the synthesis of specific glycan structures essential for virus replication and infection
Acanthamoeba polyphaga Mimivirus
evolution
phylogenetic analysis indicates that ATCV-1 has probably acquired its UGD gene via a recent horizontal gene transfer from a green algal host, while an earlier event involving the complete pathway probably occurred between a protozoan ancestor and mimivirus. While ATCV-1 lacks an epimerase/reductase gene, its Chlorella host may encode this enzyme. Chloroviruses, and maybe mimivirus, may encode most, if not all, of the glycosylation machinery involved in the synthesis of specific glycan structures essential for virus replication and infection
Acanthocystis turfacea chlorella virus 1
General Information (protein specific)
General Information
Commentary
Organism
evolution
phylogenetic analysis indicates that ATCV-1 has probably acquired its UGD gene via a recent horizontal gene transfer from a green algal host, while an earlier event involving the complete pathway probably occurred between a protozoan ancestor and mimivirus. Chloroviruses, and maybe mimivirus, may encode most, if not all, of the glycosylation machinery involved in the synthesis of specific glycan structures essential for virus replication and infection
Acanthamoeba polyphaga Mimivirus
evolution
phylogenetic analysis indicates that ATCV-1 has probably acquired its UGD gene via a recent horizontal gene transfer from a green algal host, while an earlier event involving the complete pathway probably occurred between a protozoan ancestor and mimivirus. While ATCV-1 lacks an epimerase/reductase gene, its Chlorella host may encode this enzyme. Chloroviruses, and maybe mimivirus, may encode most, if not all, of the glycosylation machinery involved in the synthesis of specific glycan structures essential for virus replication and infection
Acanthocystis turfacea chlorella virus 1
Other publictions for EC 4.2.1.76
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730005
Martinez
Biosynthesis of UDP-4-keto-6-d ...
Botrytis cinerea, Botrytis cinerea B05.10, Magnaporthe grisea, Magnaporthe grisea CP987
J. Biol. Chem.
287
879-892
2012
-
-
2
-
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2
-
-
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4
4
-
12
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2
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-
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8
2
2
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-
2
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2
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2
2
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2
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4
4
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2
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8
2
2
-
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2
-
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-
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-
730794
Sen
UDP-glucose 4, 6-dehydratase a ...
Candida albicans
PLoS Pathog.
7
e1002384
2011
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1
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5
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1
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1
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-
-
-
-
2
2
-
-
-
716065
Parakkottil Chothi
Identification of an L-rhamnos ...
Acanthamoeba polyphaga Mimivirus, Acanthocystis turfacea chlorella virus 1
J. Virol.
84
8829-8838
2010
1
-
2
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2
1
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-
6
4
-
10
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1
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2
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8
3
2
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2
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2
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1
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2
1
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4
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3
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2
2
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680873
Oka
Functional analysis of Arabido ...
Arabidopsis thaliana
J. Biol. Chem.
282
5389-5403
2007
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1
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5
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4
1
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1
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4
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1
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1
1
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5
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1
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1
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1
1
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1
1
1
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5892
Kamsteeg
The formation of UDP-L-rhamnos ...
Silene dioica
FEBS Lett.
91
281-284
1978
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1
1
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