Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant enzymes in Escherichia coli | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D17A | 60.3% of wild-type activity | Rattus norvegicus |
E68A | 82.8% of wild-type activity | Rattus norvegicus |
H173L | 106.9% of wild-type activity | Rattus norvegicus |
K10Q | 39.7% of wild-type activity | Rattus norvegicus |
K147Q | 34.5% of wild-type activity | Rattus norvegicus |
N121D | 93.1% of wild-type activity | Rattus norvegicus |
Y168F | 1.7% of wild-type activity | Rattus norvegicus |
Y97F | 74.1% of wild-type activity | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | optimal concentration is 100 mM, wild-type enzyme | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.7 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C, stable for at least one year | Rattus norvegicus |
4 °C, stable for one week without significant change of activity | Rattus norvegicus |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
in both phosphate and Tris buffer | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | pH 7.5: about 60% of maximal activity, pH 9.0: about 70% of maximal activity | Rattus norvegicus |