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Literature summary for 4.2.1.7 extracted from

  • Smiley, J.D.; Ashwell, G.
    Uronic acid metabolism in bacteria. III. Purification and properties of D-altronic acid and D-mannonic acid dehydrases in Escherichia coli (1960), J. Biol. Chem., 235, 1571-1575.
    View publication on PubMed
Search for more literature for 4.2.1.7

Activating Compound

Activating Compound Comment Organism Structure
Cys increases activity Escherichia coli

General Stability

General Stability Organism
addition of high concentrations, 0.1 M, of Cys or mercaptoethanol and maintenance in pH range pH 7.0-8.0 stabilizes throughout purification Escherichia coli
freezing, agitation or stirring in the presence of air or dialysis at pH levels below pH 7.0 inactivates Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Escherichia coli
PCMB
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ optimal activation at 0.2 mM or greater Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Storage Stability

Storage Stability Organism
0-5°C, loss of activity after 2-3 weeks Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-altronate
-
 Escherichia coli 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.2
-
 Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 dialysis below pH 7 inactivates Escherichia coli