Cloned (Comment) | Organism |
---|---|
overexpression of the wild-type and mutant enzymes in Escherichia coli XL1-Blue | Fusarium lateritium |
Protein Variants | Comment | Organism |
---|---|---|
D275E | site-directed mutagenesis, 0-29% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
F170L | site-directed mutagenesis, inactive mutant compared to the wild-type enzyme | Fusarium lateritium |
K136R | site-directed mutagenesis, 52% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
M302S | site-directed mutagenesis, 22% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
S13A | site-directed mutagenesis, 19-47% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
T12P | site-directed mutagenesis, inactive mutant | Fusarium lateritium |
T12Q | site-directed mutagenesis, 10-48% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
V281A | site-directed mutagenesis, 8% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium lateritium | P32963 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
formamide = cyanide + H2O | Phe170 is an active site residue | Fusarium lateritium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
cyanide hydratase and nitrilase activity | Fusarium lateritium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetonitrile + H2O | - |
Fusarium lateritium | ? | - |
? | |
benzonitrile + H2O | best substrate for nitrilase activity | Fusarium lateritium | ? | - |
? | |
HCN + H2O | - |
Fusarium lateritium | formamide | - |
? | |
additional information | the enzyme also shows low nitrilase activity, which is sufficient for the fungus to grow on acetonitrile, propionitrile, or benzonitrile as sole nitrogen source | Fusarium lateritium | ? | - |
? | |
propionitrile + H2O | - |
Fusarium lateritium | ? | - |
? |