BRENDA - Enzyme Database show
show all sequences of 4.2.1.65

Metabolism of nitriles in microorganisms. Part V. Purification, crystallization and some properties of beta-cyano-L-alanine-degrading enzyme in Pseudomonas sp. 13

Yanase, H.; Sakai, T.; Tonomura, K.; Agric. Biol. Chem. 47, 473-482 (1983)
No PubMed abstract available

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
-
Pseudomonas sp.
General Stability
General Stability
Organism
very unstable, dithiothreitol, GSH and 2-mercaptoethanol protect
Pseudomonas sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
2,2'-dipyridyl
poor
Pseudomonas sp.
Ag+
the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol
Pseudomonas sp.
Cd2+
the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol
Pseudomonas sp.
Cu2+
the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol
Pseudomonas sp.
HgCl2
the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol
Pseudomonas sp.
iodoacetic acid
poor
Pseudomonas sp.
Mersalyl acid
-
Pseudomonas sp.
N-ethylmaleimide
poor
Pseudomonas sp.
NaF
poor
Pseudomonas sp.
NaNO2
poor
Pseudomonas sp.
p-chloromercuribenzoate
-
Pseudomonas sp.
p-chloromercuriphenyl sulfonic acid
-
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
83
-
Beta-cyano-L-alanine
-
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
x * 35000, SDS-PAGE
Pseudomonas sp.
1000000
-
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-Cyano-L-alanine + H2O
Pseudomonas sp.
cyanide assimilation
?
-
-
-
beta-Cyano-L-alanine + H2O
Pseudomonas sp. 13
cyanide assimilation
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
13
-
Pseudomonas sp. 13
-
13
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
54.7
-
-
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-Cyano-L-alanine + H2O
highly specific for beta-cyano-L-alanine
5824
Pseudomonas sp.
L-Asparagine
-
5824
Pseudomonas sp.
-
beta-Cyano-L-alanine + H2O
highly specific for beta-cyano-L-alanine
5824
Pseudomonas sp. 13
L-Asparagine
-
5824
Pseudomonas sp. 13
-
beta-Cyano-L-alanine + H2O
cyanide assimilation
5824
Pseudomonas sp.
?
-
-
-
-
beta-Cyano-L-alanine + H2O
cyanide assimilation
5824
Pseudomonas sp. 13
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 35000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Pseudomonas sp.
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
65
-
Pseudomonas sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
up to, 10 min
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Pseudomonas sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
11
-
Pseudomonas sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7
11
in presence of 2-mercaptoethanol
Pseudomonas sp.
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Pseudomonas sp.
General Stability (protein specific)
General Stability
Organism
very unstable, dithiothreitol, GSH and 2-mercaptoethanol protect
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,2'-dipyridyl
poor
Pseudomonas sp.
Ag+
the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol
Pseudomonas sp.
Cd2+
the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol
Pseudomonas sp.
Cu2+
the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol
Pseudomonas sp.
HgCl2
the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol
Pseudomonas sp.
iodoacetic acid
poor
Pseudomonas sp.
Mersalyl acid
-
Pseudomonas sp.
N-ethylmaleimide
poor
Pseudomonas sp.
NaF
poor
Pseudomonas sp.
NaNO2
poor
Pseudomonas sp.
p-chloromercuribenzoate
-
Pseudomonas sp.
p-chloromercuriphenyl sulfonic acid
-
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
83
-
Beta-cyano-L-alanine
-
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
x * 35000, SDS-PAGE
Pseudomonas sp.
1000000
-
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-Cyano-L-alanine + H2O
Pseudomonas sp.
cyanide assimilation
?
-
-
-
beta-Cyano-L-alanine + H2O
Pseudomonas sp. 13
cyanide assimilation
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
54.7
-
-
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-Cyano-L-alanine + H2O
highly specific for beta-cyano-L-alanine
5824
Pseudomonas sp.
L-Asparagine
-
5824
Pseudomonas sp.
-
beta-Cyano-L-alanine + H2O
highly specific for beta-cyano-L-alanine
5824
Pseudomonas sp. 13
L-Asparagine
-
5824
Pseudomonas sp. 13
-
beta-Cyano-L-alanine + H2O
cyanide assimilation
5824
Pseudomonas sp.
?
-
-
-
-
beta-Cyano-L-alanine + H2O
cyanide assimilation
5824
Pseudomonas sp. 13
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 35000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Pseudomonas sp.
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
65
-
Pseudomonas sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
up to, 10 min
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Pseudomonas sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
11
-
Pseudomonas sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7
11
in presence of 2-mercaptoethanol
Pseudomonas sp.
Other publictions for EC 4.2.1.65
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
670571
Piotrowski
Cyanide metabolism in higher p ...
Lupinus angustifolius
Plant Mol. Biol.
61
111-122
2006
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5824
Yanase
-
Metabolism of nitriles in micr ...
Pseudomonas sp., Pseudomonas sp. 13
Agric. Biol. Chem.
47
473-482
1983
-
-
-
1
-
1
12
1
-
-
2
2
-
3
-
-
1
-
-
-
1
-
4
1
1
1
1
-
1
1
1
-
-
-
-
-
-
-
-
1
-
1
-
12
-
1
-
-
2
2
-
-
-
1
-
-
1
-
4
1
1
1
1
-
1
1
1
-
-
-
-
-
-
-
5825
Yanase
-
Microbial utilization of beta- ...
Pseudomonas sp., Pseudomonas sp. 13
Agric. Biol. Chem.
46
2925-2932
1982
-
-
-
-
-
2
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5826
Castric
Cyanide metabolism in higher p ...
Lupinus angustifolius, Sorghum bicolor
Arch. Biochem. Biophys.
152
62-69
1972
-
-
-
-
-
-
6
1
1
-
1
-
-
2
-
-
1
-
-
4
2
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
1
1
-
1
-
-
-
-
1
-
4
2
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
5827
Castric
Cyanide metabolism by Bacillus ...
Bacillus megaterium
J. Biol. Chem.
244
4089-4094
1969
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-