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Literature summary for 4.2.1.6 extracted from
- D-Galactonate dehydratase (1982), Methods Enzymol., 90, 294-298.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | - |
Pseudomonas sp. |  |
| PCMB | - |
Pseudomonas sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.14 | - |
D-galactonate | - |
Pseudomonas sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | optimal concentration: 1 mM | Pseudomonas sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 240000 | 245000 | - |
Pseudomonas sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactonate | Pseudomonas sp. | induced by growth on D-galactose, D-galactonate, D-fucose, and L-arabinose over the level present in glucose-grown cells | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | - |
ATCC 27855 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas sp. |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H2O | the immediate dehydration product is the enol which undergoes spontaneous ketonization | Pseudomonas sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 38 | - |
- |
Pseudomonas sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.05 M glycine-0.01 M sodium cacodylate, pH 8.8, stable for 6 months | Pseudomonas sp. |
| -20°C, 6 months, stable | Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactonate | induced by growth on D-galactose, D-galactonate, D-fucose, and L-arabinose over the level present in glucose-grown cells | Pseudomonas sp. | ? | - |
? | |
| D-galactonate | the enzyme is strictly specific for D-galactonate | Pseudomonas sp. | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Pseudomonas sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | - |
- |
Pseudomonas sp. |
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Release 2023.1 (January 2023)
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