BRENDA - Enzyme Database
show all sequences of 4.2.1.59

Intermediate- and long-chain beta-hydroxyacyl-ACP dehydratases from E. coli fatty acid synthetase

Mizugaki, M.; Swindell, A.C.; Wakil, S.J.; Biochem. Biophys. Res. Commun. 33, 520-527 (1968) View publication on PubMed

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
p-chloromercuribenzoate
inhibition at 0.1 mM
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(3R)-3-hydroxydodecanoyl-[acyl-carrier protein]
Escherichia coli
-
2-dodecenoyl-[acyl-carrier protein] + H2O
-
-
?
additional information
Escherichia coli
3-Hydroxyacyl-[acyl-carrier protein] derivatives, C6 to C12
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.089
-
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(3R)-3-hydroxydecanoyl-[acyl-carrier protein]
-
5799
Escherichia coli
2-decenoyl-[acyl-carrier protein] + H2O
-
-
-
?
(3R)-3-hydroxydodecanoyl-[acyl-carrier protein]
-
5799
Escherichia coli
2-dodecenoyl-[acyl-carrier protein] + H2O
-
-
-
?
(3R)-3-hydroxyheptanoyl-[acyl-carrier protein]
-
5799
Escherichia coli
2-heptenoyl-[acyl-carrier protein] + H2O
-
-
-
?
(3R)-3-hydroxyhexanoyl-[acyl-carrier protein]
-
5799
Escherichia coli
2-hexenoyl-[acyl-carrier protein] + H2O
-
-
-
?
(3R)-3-hydroxynonanoyl-[acyl-carrier protein]
-
5799
Escherichia coli
2-nonenoyl-[acyl-carrier protein] + H2O
-
-
-
?
(3R)-3-hydroxyoctanoyl-[acyl-carrier protein]
beta-hydroxyoctanoyl-ACP
5799
Escherichia coli
2-octenoyl-[acyl-carrier protein] + H2O
-
-
-
?
(3R)-3-hydroxyundecanoyl-[acyl-carrier protein]
-
5799
Escherichia coli
2-undecenoyl-[acyl-carrier protein] + H2O
-
-
-
?
additional information
no activity on C14- or C16-ACP derivatives
5799
Escherichia coli
?
-
-
-
?
additional information
specific for 3-hydroxyacyl-[acyl-carrier protein] derivatives, C6 to C12, highest activity on C8 derivative
5799
Escherichia coli
?
-
-
-
?
additional information
3-Hydroxyacyl-[acyl-carrier protein] derivatives, C6 to C12
5799
Escherichia coli
?
-
-
-
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
65
20-30% loss of activity after 5 min
Escherichia coli
70
-
90% loss of activity after 5 min
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
enzyme assay at
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
p-chloromercuribenzoate
inhibition at 0.1 mM
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(3R)-3-hydroxydodecanoyl-[acyl-carrier protein]
Escherichia coli
-
2-dodecenoyl-[acyl-carrier protein] + H2O
-
-
?
additional information
Escherichia coli
3-Hydroxyacyl-[acyl-carrier protein] derivatives, C6 to C12
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.089
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(3R)-3-hydroxydecanoyl-[acyl-carrier protein]
-
5799
Escherichia coli
2-decenoyl-[acyl-carrier protein] + H2O
-
-
-
?
(3R)-3-hydroxydodecanoyl-[acyl-carrier protein]
-
5799
Escherichia coli
2-dodecenoyl-[acyl-carrier protein] + H2O
-
-
-
?
(3R)-3-hydroxyheptanoyl-[acyl-carrier protein]
-
5799
Escherichia coli
2-heptenoyl-[acyl-carrier protein] + H2O
-
-
-
?
(3R)-3-hydroxyhexanoyl-[acyl-carrier protein]
-
5799
Escherichia coli
2-hexenoyl-[acyl-carrier protein] + H2O
-
-
-
?
(3R)-3-hydroxynonanoyl-[acyl-carrier protein]
-
5799
Escherichia coli
2-nonenoyl-[acyl-carrier protein] + H2O
-
-
-
?
(3R)-3-hydroxyoctanoyl-[acyl-carrier protein]
beta-hydroxyoctanoyl-ACP
5799
Escherichia coli
2-octenoyl-[acyl-carrier protein] + H2O
-
-
-
?
(3R)-3-hydroxyundecanoyl-[acyl-carrier protein]
-
5799
Escherichia coli
2-undecenoyl-[acyl-carrier protein] + H2O
-
-
-
?
additional information
no activity on C14- or C16-ACP derivatives
5799
Escherichia coli
?
-
-
-
?
additional information
specific for 3-hydroxyacyl-[acyl-carrier protein] derivatives, C6 to C12, highest activity on C8 derivative
5799
Escherichia coli
?
-
-
-
?
additional information
3-Hydroxyacyl-[acyl-carrier protein] derivatives, C6 to C12
5799
Escherichia coli
?
-
-
-
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
65
20-30% loss of activity after 5 min
Escherichia coli
70
-
90% loss of activity after 5 min
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
enzyme assay at
Escherichia coli
Other publictions for EC 4.2.1.59
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748986
Guan
Discovery and characterizatio ...
Arabidopsis thaliana
Plant Physiol.
173
2010-2028
2017
-
-
1
-
-
-
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-
3
-
-
-
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6
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4
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2
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1
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3
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4
-
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1
1
-
-
-
748442
Singh
Identification and characteri ...
Streptomyces coelicolor, Streptomyces coelicolor ATCC BAA-471
J. Nat. Prod.
79
240-243
2016
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-
1
-
-
-
-
-
-
-
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3
-
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11
1
2
1
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1
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1
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11
1
1
-
-
-
1
-
-
-
-
1
1
-
6
6
749034
Gonzalez-Thuillier
Sunflower (Helianthus annuus) ...
Helianthus annuus
Planta
243
397-410
2016
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-
1
-
1
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3
-
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6
-
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1
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-
-
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1
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3
-
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1
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1
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1
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3
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1
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1
-
-
-
-
-
-
-
-
1
1
2
2
1
-
-
747496
Finzel
Probing the substrate specifi ...
Escherichia coli
Chem. Biol.
22
1453-1460
2015
-
-
-
-
3
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
2
-
-
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3
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-
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-
-
-
-
-
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-
1
1
-
-
-
730467
Nguyen
Trapping the dynamic acyl carr ...
Escherichia coli
Nature
505
427-431
2014
-
-
1
1
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
1
-
1
-
-
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1
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1
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1
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1
-
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-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5799
Mizugaki
Intermediate- and long-chain b ...
Escherichia coli
Biochem. Biophys. Res. Commun.
33
520-527
1968
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
1
-
-
-
1
-
10
-
-
-
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2
-
1
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1
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2
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1
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1
-
10
-
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2
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1
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