BRENDA - Enzyme Database show
show all sequences of 4.2.1.54

Lactyl-CoA dehydrase

Baldwin, R.L.; Wood, W.A.; Methods Enzymol. 9, 683-685 (1966)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
very unstable during purification
Megasphaera elsdenii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Megasphaera elsdenii
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Megasphaera elsdenii
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.9
-
-
Megasphaera elsdenii
Storage Stability
Storage Stability
Organism
-15°C, crude extract relatively stable
Megasphaera elsdenii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acrylyl-CoA + H2O
-
33926
Megasphaera elsdenii
lactoyl-CoA
-
33926
Megasphaera elsdenii
?
crotonoyl-CoA + H2O
-
33926
Megasphaera elsdenii
2-hydroxybutyryl-CoA
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
-
Megasphaera elsdenii
General Stability (protein specific)
General Stability
Organism
very unstable during purification
Megasphaera elsdenii
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Megasphaera elsdenii
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.9
-
-
Megasphaera elsdenii
Storage Stability (protein specific)
Storage Stability
Organism
-15°C, crude extract relatively stable
Megasphaera elsdenii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acrylyl-CoA + H2O
-
33926
Megasphaera elsdenii
lactoyl-CoA
-
33926
Megasphaera elsdenii
?
crotonoyl-CoA + H2O
-
33926
Megasphaera elsdenii
2-hydroxybutyryl-CoA
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
-
Megasphaera elsdenii
Other publictions for EC 4.2.1.54
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729862
Reichardt
Phylogenetic distribution of t ...
Anaerotignum propionicum, Anaerotignum propionicum DSM 1682, butyrate-producing bacterium SS3/4, Clostridioides difficile, Clostridioides difficile 630, Clostridium asparagiforme, Clostridium asparagiforme DSM 15981, Clostridium botulinum, Clostridium botulinum A2, Clostridium botulinum C Eklund, Clostridium novyi, Clostridium novyi NT, Clostridium sp., Clostridium sp. M62/1, Clostridium sp. MSTE9 (cluster XIVb), Clostridium symbiosum, Clostridium symbiosum WAL-14163, Coprococcus catus, Coprococcus catus GD/7, Megasphaera elsdenii, Megasphaera elsdenii LC1 DSM 20460T
ISME J.
8
1323-1335
2014
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21
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26
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21
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21
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21
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718680
Kandasamy
Engineering Escherichia coli w ...
Anaerotignum propionicum, Anaerotignum propionicum DSM 1682
Appl. Microbiol. Biotechnol.
97
1191-1200
2013
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1
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2
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4
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2
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1
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2
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33931
Brunelle
-
The stereochemistry of hydrati ...
Anaerotignum propionicum
Bioorg. Chem.
21
118-126
1993
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1
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1
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1
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1
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2
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33930
Hofmeister
(R)-lactyl-CoA dehydratase fro ...
Anaerotignum propionicum
Eur. J. Biochem.
206
547-552
1992
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1
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1
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1
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2
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33929
Kuchta
Fe-S centers in lactyl-CoA deh ...
Anaerotignum propionicum
Biochemistry
25
7301-7307
1986
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1
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1
1
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1
1
3
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1
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1
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1
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1
1
3
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33928
Schweiger
On the dehydration of (R)-lact ...
Anaerotignum propionicum
FEBS Lett.
171
79-84
1984
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1
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33926
Baldwin
-
Lactyl-CoA dehydrase ...
Megasphaera elsdenii
Methods Enzymol.
9
683-685
1966
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1
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1
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1
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1
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1
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1
1
2
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1
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33927
Baldwin
Lactate metabolism by Peptostr ...
Megasphaera elsdenii
Biochim. Biophys. Acta
97
202-213
1965
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1
3
1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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