BRENDA - Enzyme Database show
show all sequences of 4.2.1.53

Production of 10-hydroxystearic acid from oleic acid by whole cells of recombinant Escherichia coli containing oleate hydratase from Stenotrophomonas maltophilia

Joo, Y.C.; Seo, E.S.; Kim, Y.S.; Kim, K.R.; Park, J.B.; Oh, D.K.; J. Biotechnol. 158, 17-23 (2012)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Tween 40
maximum activity at 0.05% (v/v) Tween 40
Stenotrophomonas maltophilia
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli ER2566, BL21(DE3), W3110, and MLK1067 cells
Stenotrophomonas maltophilia
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.46
-
oleate
at pH 6.5 and 35C
Stenotrophomonas maltophilia
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
2 * 68000, SDS-PAGE
Stenotrophomonas maltophilia
136000
-
gel filtration
Stenotrophomonas maltophilia
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
oleate + H2O
Stenotrophomonas maltophilia
highest specific activity
(R)-10-hydroxystearate
98% yield after 4 h
-
?
oleate + H2O
Stenotrophomonas maltophilia KCTC 1773
highest specific activity
(R)-10-hydroxystearate
98% yield after 4 h
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Stenotrophomonas maltophilia
-
-
-
Stenotrophomonas maltophilia KCTC 1773
-
-
-
Purification (Commentary)
Commentary
Organism
His-Trap column chromatography
Stenotrophomonas maltophilia
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.014
-
with myristoleic acid as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
0.033
-
with gamma-linolenic acid as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
0.54
-
with alpha-linolenic acid as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
0.91
-
with linoleic acid as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
1.95
-
with palmitoleic acid as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
3.69
-
with oleate as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-linolenic acid + H2O
-
730082
Stenotrophomonas maltophilia
10-hydroxy-12,15(Z,Z)-octadecadienoic acid
-
-
-
?
alpha-linolenic acid + H2O
-
730082
Stenotrophomonas maltophilia KCTC 1773
10-hydroxy-12,15(Z,Z)-octadecadienoic acid
-
-
-
?
gamma-linolenic acid + H2O
-
730082
Stenotrophomonas maltophilia
10-hydroxy-6,12(Z,Z)-octadecadienoic acid
-
-
-
?
gamma-linolenic acid + H2O
-
730082
Stenotrophomonas maltophilia KCTC 1773
10-hydroxy-6,12(Z,Z)-octadecadienoic acid
-
-
-
?
linoleic acid + H2O
-
730082
Stenotrophomonas maltophilia
10-hydroxy-12(Z)-octadecenoic acid
-
-
-
?
additional information
no hydration activity for myristic acid, palmitic acid, stearic acid, arachidic acid, petroselinic acid, elaidic acid, vaccenic acid, conjugated linoleic acids, arachidonic acid, erucic ac, and nervonic acid
730082
Stenotrophomonas maltophilia
?
-
-
-
-
additional information
no hydration activity for myristic acid, palmitic acid, stearic acid, arachidic acid, petroselinic acid, elaidic acid, vaccenic acid, conjugated linoleic acids, arachidonic acid, erucic ac, and nervonic acid
730082
Stenotrophomonas maltophilia KCTC 1773
?
-
-
-
-
myristoleic acid + H2O
-
730082
Stenotrophomonas maltophilia
10-hydroxymyristic acid
-
-
-
?
oleate + H2O
highest specific activity
730082
Stenotrophomonas maltophilia
(R)-10-hydroxystearate
98% yield after 4 h
-
-
?
oleate + H2O
highest specific activity
730082
Stenotrophomonas maltophilia KCTC 1773
(R)-10-hydroxystearate
98% yield after 4 h
-
-
?
Palmitoleic acid + H2O
-
730082
Stenotrophomonas maltophilia
10-Hydroxypalmitic acid
-
-
-
?
Palmitoleic acid + H2O
-
730082
Stenotrophomonas maltophilia KCTC 1773
10-Hydroxypalmitic acid
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 68000, SDS-PAGE
Stenotrophomonas maltophilia
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
35
-
-
Stenotrophomonas maltophilia
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
11.83
-
oleate
at pH 6.5 and 35C
Stenotrophomonas maltophilia
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Stenotrophomonas maltophilia
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
the addition of FAD (0.01-0.2 mM) has no effect on the activity
Stenotrophomonas maltophilia
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Tween 40
maximum activity at 0.05% (v/v) Tween 40
Stenotrophomonas maltophilia
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli ER2566, BL21(DE3), W3110, and MLK1067 cells
Stenotrophomonas maltophilia
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
the addition of FAD (0.01-0.2 mM) has no effect on the activity
Stenotrophomonas maltophilia
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.46
-
oleate
at pH 6.5 and 35C
Stenotrophomonas maltophilia
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
2 * 68000, SDS-PAGE
Stenotrophomonas maltophilia
136000
-
gel filtration
Stenotrophomonas maltophilia
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
oleate + H2O
Stenotrophomonas maltophilia
highest specific activity
(R)-10-hydroxystearate
98% yield after 4 h
-
?
oleate + H2O
Stenotrophomonas maltophilia KCTC 1773
highest specific activity
(R)-10-hydroxystearate
98% yield after 4 h
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
His-Trap column chromatography
Stenotrophomonas maltophilia
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.014
-
with myristoleic acid as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
0.033
-
with gamma-linolenic acid as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
0.54
-
with alpha-linolenic acid as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
0.91
-
with linoleic acid as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
1.95
-
with palmitoleic acid as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
3.69
-
with oleate as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-linolenic acid + H2O
-
730082
Stenotrophomonas maltophilia
10-hydroxy-12,15(Z,Z)-octadecadienoic acid
-
-
-
?
alpha-linolenic acid + H2O
-
730082
Stenotrophomonas maltophilia KCTC 1773
10-hydroxy-12,15(Z,Z)-octadecadienoic acid
-
-
-
?
gamma-linolenic acid + H2O
-
730082
Stenotrophomonas maltophilia
10-hydroxy-6,12(Z,Z)-octadecadienoic acid
-
-
-
?
gamma-linolenic acid + H2O
-
730082
Stenotrophomonas maltophilia KCTC 1773
10-hydroxy-6,12(Z,Z)-octadecadienoic acid
-
-
-
?
linoleic acid + H2O
-
730082
Stenotrophomonas maltophilia
10-hydroxy-12(Z)-octadecenoic acid
-
-
-
?
additional information
no hydration activity for myristic acid, palmitic acid, stearic acid, arachidic acid, petroselinic acid, elaidic acid, vaccenic acid, conjugated linoleic acids, arachidonic acid, erucic ac, and nervonic acid
730082
Stenotrophomonas maltophilia
?
-
-
-
-
additional information
no hydration activity for myristic acid, palmitic acid, stearic acid, arachidic acid, petroselinic acid, elaidic acid, vaccenic acid, conjugated linoleic acids, arachidonic acid, erucic ac, and nervonic acid
730082
Stenotrophomonas maltophilia KCTC 1773
?
-
-
-
-
myristoleic acid + H2O
-
730082
Stenotrophomonas maltophilia
10-hydroxymyristic acid
-
-
-
?
oleate + H2O
highest specific activity
730082
Stenotrophomonas maltophilia
(R)-10-hydroxystearate
98% yield after 4 h
-
-
?
oleate + H2O
highest specific activity
730082
Stenotrophomonas maltophilia KCTC 1773
(R)-10-hydroxystearate
98% yield after 4 h
-
-
?
Palmitoleic acid + H2O
-
730082
Stenotrophomonas maltophilia
10-Hydroxypalmitic acid
-
-
-
?
Palmitoleic acid + H2O
-
730082
Stenotrophomonas maltophilia KCTC 1773
10-Hydroxypalmitic acid
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 68000, SDS-PAGE
Stenotrophomonas maltophilia
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
35
-
-
Stenotrophomonas maltophilia
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
11.83
-
oleate
at pH 6.5 and 35C
Stenotrophomonas maltophilia
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Stenotrophomonas maltophilia
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
25.67
-
oleate
at pH 6.5 and 35C
Stenotrophomonas maltophilia
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
25.67
-
oleate
at pH 6.5 and 35C
Stenotrophomonas maltophilia
Other publictions for EC 4.2.1.53
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747558
Lorenzen
-
Rhodococcus erythropolis olea ...
Rhodococcus erythropolis, Rhodococcus erythropolis DN1
ChemCatChem
10
407-414
2018
-
-
1
1
2
-
-
1
-
1
-
-
-
2
-
-
1
-
-
-
5
-
11
1
1
1
-
1
1
1
-
1
-
-
-
-
-
1
1
1
2
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
5
-
11
1
1
1
-
1
1
1
-
-
-
-
-
-
-
-
747692
Fibinger
-
A directed mutational approac ...
Lactobacillus acidophilus
Eur. J. Lipid Sci. Technol.
118
841-848
2016
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
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-
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-
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1
-
1
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-
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-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
748416
Schmid
-
Biocatalytic study of novel o ...
Chryseobacterium gleum, Chryseobacterium gleum ATCC 35910, Desulfomicrobium baculatum, Desulfomicrobium baculatum DSM 4028, Elizabethkingia meningoseptica, Gemella morbillorum, Gemella morbillorum M424, Haloterrigena thermotolerans, Haloterrigena thermotolerans DSM 11522, Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396, Lactobacillus johnsonii, Mucilaginibacter paludis, Mucilaginibacter paludis DSM 18603, Thermoanaerobacterium thermosaccharolyticum, Thermoanaerobacterium thermosaccharolyticum DSM 571
J. Mol. Catal. B
133
S243-S249
2016
-
-
9
-
-
-
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
-
44
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
44
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747562
Hiseni
-
New cofactor-independent hydr ...
Bifidobacterium animalis subsp. lactis, Bifidobacterium breve, Elizabethkingia meningoseptica, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus rhamnosus, Lysinibacillus fusiformis, Macrococcus caseolyticus, Stenotrophomonas maltophilia, Streptococcus pyogenes
ChemCatChem
7
29-37
2015
-
10
10
-
-
-
-
-
-
-
10
-
-
10
-
-
-
-
-
-
-
-
33
4
-
-
-
-
-
-
-
10
-
-
-
-
10
10
10
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
33
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
748410
Todea
-
Increase of stability of olea ...
Elizabethkingia meningoseptica
J. Mol. Catal. B
119
40-47
2015
-
-
1
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
729457
Hiseni
Enzymatic hydration activity a ...
Elizabethkingia meningoseptica
Biotechnol. J.
9
814-821
2014
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
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1
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729071
Seo
Production of a novel compound ...
Lysinibacillus fusiformis
Appl. Microbiol. Biotechnol.
97
8987-8995
2013
-
-
1
-
-
-
1
-
-
-
-
1
1
2
-
-
1
-
-
-
-
-
2
-
1
-
1
-
1
-
-
-
-
-
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1
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-
-
1
-
-
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-
1
1
-
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1
-
-
-
-
2
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
729466
Oh
Production of 10-hydroxy-12,15 ...
Stenotrophomonas maltophilia
Biotechnol. Lett.
35
1487-1493
2013
3
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
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-
1
-
1
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1
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-
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3
-
1
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-
-
-
-
-
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-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
729060
Kim
Production of 10-hydroxysteari ...
Lysinibacillus fusiformis, Lysinibacillus fusiformis KCTC 3454
Appl. Microbiol. Biotechnol.
95
929-937
2012
1
-
1
-
-
-
1
4
-
-
3
2
-
3
-
-
1
-
-
1
6
-
11
1
1
1
-
4
1
1
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
4
-
-
3
2
-
-
-
1
-
1
6
-
11
1
1
1
-
4
1
1
-
-
-
-
-
-
4
4
729334
Joo
Biochemical characterization a ...
Macrococcus caseolyticus, Macrococcus caseolyticus JCSC5402
Biochimie
94
907-915
2012
1
-
1
-
5
-
10
6
-
-
3
2
-
4
-
-
1
-
-
-
1
-
12
1
1
1
1
6
1
1
-
2
-
-
-
1
-
1
2
-
5
-
-
10
-
6
-
-
3
2
-
-
-
1
-
-
1
-
12
1
1
1
1
6
1
1
-
-
-
-
-
-
6
6
730082
Joo
Production of 10-hydroxysteari ...
Stenotrophomonas maltophilia, Stenotrophomonas maltophilia KCTC 1773
J. Biotechnol.
158
17-23
2012
1
-
1
-
-
-
-
1
-
-
2
2
-
4
-
-
1
-
-
-
6
-
12
1
1
-
-
1
1
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
1
-
-
2
2
-
-
-
1
-
-
6
-
12
1
1
-
-
1
1
-
-
-
-
-
-
-
1
1
730845
Jeon
-
Bioprocess engineering to prod ...
Stenotrophomonas maltophilia
Process Biochem.
47
941-947
2012
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
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