Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanol | maximum activity in the presence of 2% (v/v) ethanol | Macrococcus caseolyticus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ER2566 cells | Macrococcus caseolyticus |
Protein Variants | Comment | Organism |
---|---|---|
E50A | the mutant shows reduced activity and FAD binding compared to the wild type enzyme | Macrococcus caseolyticus |
E56A | inactive | Macrococcus caseolyticus |
G29A | inactive | Macrococcus caseolyticus |
G31A | inactive | Macrococcus caseolyticus |
S34A | the mutant shows reduced activity and FAD binding compared to the wild type enzyme | Macrococcus caseolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Macrococcus caseolyticus | |
Ca2+ | - |
Macrococcus caseolyticus | |
Co2+ | - |
Macrococcus caseolyticus | |
Cu2+ | - |
Macrococcus caseolyticus | |
Fe2+ | - |
Macrococcus caseolyticus | |
Mg2+ | - |
Macrococcus caseolyticus | |
Mn2+ | - |
Macrococcus caseolyticus | |
additional information | not inhibited by EDTA | Macrococcus caseolyticus | |
Ni2+ | - |
Macrococcus caseolyticus | |
Zn2+ | - |
Macrococcus caseolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.058 | - |
linoleic acid | at pH 6.5 and 25°C | Macrococcus caseolyticus | |
0.059 | - |
alpha-linolenic acid | at pH 6.5 and 25°C | Macrococcus caseolyticus | |
0.3 | - |
myristoleic acid | at pH 6.5 and 25°C | Macrococcus caseolyticus | |
0.34 | - |
oleic acid | at pH 6.5 and 25°C | Macrococcus caseolyticus | |
0.57 | - |
palmitoleic acid | at pH 6.5 and 25°C | Macrococcus caseolyticus | |
1.12 | - |
gamma-linolenic acid | at pH 6.5 and 25°C | Macrococcus caseolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
2 * 68000, SDS-PAGE | Macrococcus caseolyticus |
68100 | - |
2 * 68100, calculated from amino acid sequence | Macrococcus caseolyticus |
136000 | - |
gel filtration | Macrococcus caseolyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oleic acid + H2O | Macrococcus caseolyticus | - |
10-hydroxyoctadecanoic acid | - |
ir | |
oleic acid + H2O | Macrococcus caseolyticus JCSC5402 | - |
10-hydroxyoctadecanoic acid | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macrococcus caseolyticus | B9E972 | - |
- |
Macrococcus caseolyticus JCSC5402 | B9E972 | - |
- |
Purification (Comment) | Organism |
---|---|
His-Trap column chromatography | Macrococcus caseolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.3 | - |
at pH 6.5 and 25°C | Macrococcus caseolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-linolenic acid + H2O | - |
Macrococcus caseolyticus | 10-hydroxy-12(Z),15(Z)-octadecadienoic acid + 10,13-dihydroxy-15(Z)-octadecenoic acid | - |
? | |
alpha-linolenic acid + H2O | - |
Macrococcus caseolyticus JCSC5402 | 10-hydroxy-12(Z),15(Z)-octadecadienoic acid + 10,13-dihydroxy-15(Z)-octadecenoic acid | - |
? | |
gamma-linolenic acid | - |
Macrococcus caseolyticus | 10-hydroxy-6(Z),12(Z)-octadecadienoic acid + 10,13-dihydroxy-6(Z)-octadecenoic acid | - |
? | |
gamma-linolenic acid | - |
Macrococcus caseolyticus JCSC5402 | 10-hydroxy-6(Z),12(Z)-octadecadienoic acid + 10,13-dihydroxy-6(Z)-octadecenoic acid | - |
? | |
linoleic acid | - |
Macrococcus caseolyticus | 10-hydroxy-12(Z)-octadecenoic acid + 10,13-dihydroxyoctadecanoic acid | - |
? | |
linoleic acid | - |
Macrococcus caseolyticus JCSC5402 | 10-hydroxy-12(Z)-octadecenoic acid + 10,13-dihydroxyoctadecanoic acid | - |
? | |
additional information | no activity with 10-hydroxyoctadecanoic acid | Macrococcus caseolyticus | ? | - |
? | |
additional information | no activity with 10-hydroxyoctadecanoic acid | Macrococcus caseolyticus JCSC5402 | ? | - |
? | |
myristoleic acid | - |
Macrococcus caseolyticus | 10-hydroxytetradecanoic acid + H2O | - |
? | |
oleic acid + H2O | - |
Macrococcus caseolyticus | 10-hydroxyoctadecanoic acid | - |
ir | |
oleic acid + H2O | - |
Macrococcus caseolyticus JCSC5402 | 10-hydroxyoctadecanoic acid | - |
ir | |
palmitoleic acid | - |
Macrococcus caseolyticus | 10-hydroxyhexadecanoic acid + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 68000, SDS-PAGE | Macrococcus caseolyticus |
homodimer | 2 * 68100, calculated from amino acid sequence | Macrococcus caseolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Macrococcus caseolyticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 35 | 40% activity at 15°C, 75% activity at 20°C, 100% activity at 25°C, 78% activity at 30°C, and 65% activity at 35°C | Macrococcus caseolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 35 | after 120 min, the enzyme activity is nearly unchanges at 25°C, but is reduced to only 25% at 35 °C | Macrococcus caseolyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
myristoleic acid | at pH 6.5 and 25°C | Macrococcus caseolyticus | |
0.13 | - |
linoleic acid | at pH 6.5 and 25°C | Macrococcus caseolyticus | |
0.13 | - |
alpha-linolenic acid | at pH 6.5 and 25°C | Macrococcus caseolyticus | |
2.67 | - |
gamma-linolenic acid | at pH 6.5 and 25°C | Macrococcus caseolyticus | |
3.83 | - |
palmitoleic acid | at pH 6.5 and 25°C | Macrococcus caseolyticus | |
7.83 | - |
oleic acid | at pH 6.5 and 25°C | Macrococcus caseolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Macrococcus caseolyticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | 48% activity at pH 5.5, 68% activity at pH 6.0, 100% activity at pH 6.5, 40% activity at pH 7.0, and 20% activity at pH 7.5 | Macrococcus caseolyticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | required for activity, maximum activity in the presence of 0.2 M FAD | Macrococcus caseolyticus | |
additional information | the addition of the cofactor FMN, NAD+, or NADP+ does not affect enzyme activity | Macrococcus caseolyticus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
myristoleic acid | at pH 6.5 and 25°C | Macrococcus caseolyticus | |
0.33 | - |
oleic acid | at pH 6.5 and 25°C | Macrococcus caseolyticus | |
0.33 | - |
linoleic acid | at pH 6.5 and 25°C | Macrococcus caseolyticus | |
0.42 | - |
alpha-linolenic acid | at pH 6.5 and 25°C | Macrococcus caseolyticus | |
4.5 | - |
gamma-linolenic acid | at pH 6.5 and 25°C | Macrococcus caseolyticus | |
22.67 | - |
palmitoleic acid | at pH 6.5 and 25°C | Macrococcus caseolyticus |