BRENDA - Reference to 4.2.1.53; Id = 729334

ethanol

maximum activity in the presence of 2% (v/v) ethanol

Macrococcus caseolyticus

expressed in Escherichia coli ER2566 cells

Macrococcus caseolyticus

E50A

the mutant shows reduced activity and FAD binding compared to the wild type enzyme

Macrococcus caseolyticus

E56A

inactive

Macrococcus caseolyticus

G29A

inactive

Macrococcus caseolyticus

G31A

inactive

Macrococcus caseolyticus

S34A

the mutant shows reduced activity and FAD binding compared to the wild type enzyme

Macrococcus caseolyticus

Ba2+

-

Macrococcus caseolyticus

Ca2+

-

Macrococcus caseolyticus

Co2+

-

Macrococcus caseolyticus

Cu2+

-

Macrococcus caseolyticus

Fe2+

-

Macrococcus caseolyticus

Mg2+

-

Macrococcus caseolyticus

Mn2+

-

Macrococcus caseolyticus

additional information

not inhibited by EDTA

Macrococcus caseolyticus

Ni2+

-

Macrococcus caseolyticus

Zn2+

-

Macrococcus caseolyticus

0.058

-

linoleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

0.059

-

alpha-linolenic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

0.3

-

myristoleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

0.34

-

oleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

0.57

-

palmitoleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

1.12

-

gamma-linolenic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

68000

-

2 * 68000, SDS-PAGE

Macrococcus caseolyticus

68100

-

2 * 68100, calculated from amino acid sequence

Macrococcus caseolyticus

136000

-

gel filtration

Macrococcus caseolyticus

oleic acid + H2O

Macrococcus caseolyticus

-

10-hydroxyoctadecanoic acid

-

ir

oleic acid + H2O

Macrococcus caseolyticus JCSC5402

-

10-hydroxyoctadecanoic acid

-

ir

Macrococcus caseolyticus

B9E972

-

Macrococcus caseolyticus JCSC5402

B9E972

-

His-Trap column chromatography

Macrococcus caseolyticus

3.3

-

at pH 6.5 and 25°C

Macrococcus caseolyticus

alpha-linolenic acid

-

729334

Macrococcus caseolyticus

10-hydroxy-12(Z),15(Z)-octadecadienoic acid + 10,13-dihydroxy-15(Z)-octadecenoic acid

-

alpha-linolenic acid

-

729334

Macrococcus caseolyticus JCSC5402

10-hydroxy-12(Z),15(Z)-octadecadienoic acid + 10,13-dihydroxy-15(Z)-octadecenoic acid

-

gamma-linolenic acid

-

729334

Macrococcus caseolyticus

10-hydroxy-6(Z),12(Z)-octadecadienoic acid + 10,13-dihydroxy-6(Z)-octadecenoic acid

-

gamma-linolenic acid

-

729334

Macrococcus caseolyticus JCSC5402

10-hydroxy-6(Z),12(Z)-octadecadienoic acid + 10,13-dihydroxy-6(Z)-octadecenoic acid

-

linoleic acid

-

729334

Macrococcus caseolyticus

10-hydroxy-12(Z)-octadecenoic acid + 10,13-dihydroxyoctadecanoic acid

-

?

linoleic acid

-

729334

Macrococcus caseolyticus JCSC5402

10-hydroxy-12(Z)-octadecenoic acid + 10,13-dihydroxyoctadecanoic acid

-

?

additional information

no activity with 10-hydroxyoctadecanoic acid

729334

Macrococcus caseolyticus

?

-

additional information

no activity with 10-hydroxyoctadecanoic acid

729334

Macrococcus caseolyticus JCSC5402

?

-

myristoleic acid

-

729334

Macrococcus caseolyticus

10-hydroxytetradecanoic acid + H2O

-

?

oleic acid + H2O

-

729334

Macrococcus caseolyticus

10-hydroxyoctadecanoic acid

-

ir

oleic acid + H2O

-

729334

Macrococcus caseolyticus JCSC5402

10-hydroxyoctadecanoic acid

-

ir

palmitoleic acid

-

729334

Macrococcus caseolyticus

10-hydroxyhexadecanoic acid + H2O

-

?

homodimer

2 * 68000, SDS-PAGE; 2 * 68100, calculated from amino acid sequence

Macrococcus caseolyticus

25

-

Macrococcus caseolyticus

15

35

40% activity at 15°C, 75% activity at 20°C, 100% activity at 25°C, 78% activity at 30°C, and 65% activity at 35°C

Macrococcus caseolyticus

25

35

after 120 min, the enzyme activity is nearly unchanges at 25°C, but is reduced to only 25% at 35 °C

Macrococcus caseolyticus

0.03

-

myristoleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

0.13

-

alpha-linolenic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

0.13

-

linoleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

2.67

-

gamma-linolenic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

3.83

-

palmitoleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

7.83

-

oleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

6.5

-

Macrococcus caseolyticus

5.5

7.5

48% activity at pH 5.5, 68% activity at pH 6.0, 100% activity at pH 6.5, 40% activity at pH 7.0, and 20% activity at pH 7.5

Macrococcus caseolyticus

FAD

required for activity, maximum activity in the presence of 0.2 M FAD

Macrococcus caseolyticus

additional information

the addition of the cofactor FMN, NAD+, or NADP+ does not affect enzyme activity

Macrococcus caseolyticus

ethanol

maximum activity in the presence of 2% (v/v) ethanol

Macrococcus caseolyticus

expressed in Escherichia coli ER2566 cells

Macrococcus caseolyticus

FAD

required for activity, maximum activity in the presence of 0.2 M FAD

Macrococcus caseolyticus

additional information

the addition of the cofactor FMN, NAD+, or NADP+ does not affect enzyme activity

Macrococcus caseolyticus

E50A

the mutant shows reduced activity and FAD binding compared to the wild type enzyme

Macrococcus caseolyticus

E56A

inactive

Macrococcus caseolyticus

G29A

inactive

Macrococcus caseolyticus

G31A

inactive

Macrococcus caseolyticus

S34A

the mutant shows reduced activity and FAD binding compared to the wild type enzyme

Macrococcus caseolyticus

Ba2+

-

Macrococcus caseolyticus

Ca2+

-

Macrococcus caseolyticus

Co2+

-

Macrococcus caseolyticus

Cu2+

-

Macrococcus caseolyticus

Fe2+

-

Macrococcus caseolyticus

Mg2+

-

Macrococcus caseolyticus

Mn2+

-

Macrococcus caseolyticus

additional information

not inhibited by EDTA

Macrococcus caseolyticus

Ni2+

-

Macrococcus caseolyticus

Zn2+

-

Macrococcus caseolyticus

0.058

-

linoleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

0.059

-

alpha-linolenic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

0.3

-

myristoleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

0.34

-

oleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

0.57

-

palmitoleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

1.12

-

gamma-linolenic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

68000

-

2 * 68000, SDS-PAGE

Macrococcus caseolyticus

68100

-

2 * 68100, calculated from amino acid sequence

Macrococcus caseolyticus

136000

-

gel filtration

Macrococcus caseolyticus

oleic acid + H2O

Macrococcus caseolyticus

-

10-hydroxyoctadecanoic acid

-

ir

oleic acid + H2O

Macrococcus caseolyticus JCSC5402

-

10-hydroxyoctadecanoic acid

-

ir

His-Trap column chromatography

Macrococcus caseolyticus

3.3

-

at pH 6.5 and 25°C

Macrococcus caseolyticus

alpha-linolenic acid

-

729334

Macrococcus caseolyticus

10-hydroxy-12(Z),15(Z)-octadecadienoic acid + 10,13-dihydroxy-15(Z)-octadecenoic acid

-

alpha-linolenic acid

-

729334

Macrococcus caseolyticus JCSC5402

10-hydroxy-12(Z),15(Z)-octadecadienoic acid + 10,13-dihydroxy-15(Z)-octadecenoic acid

-

gamma-linolenic acid

-

729334

Macrococcus caseolyticus

10-hydroxy-6(Z),12(Z)-octadecadienoic acid + 10,13-dihydroxy-6(Z)-octadecenoic acid

-

gamma-linolenic acid

-

729334

Macrococcus caseolyticus JCSC5402

10-hydroxy-6(Z),12(Z)-octadecadienoic acid + 10,13-dihydroxy-6(Z)-octadecenoic acid

-

linoleic acid

-

729334

Macrococcus caseolyticus

10-hydroxy-12(Z)-octadecenoic acid + 10,13-dihydroxyoctadecanoic acid

-

?

linoleic acid

-

729334

Macrococcus caseolyticus JCSC5402

10-hydroxy-12(Z)-octadecenoic acid + 10,13-dihydroxyoctadecanoic acid

-

?

additional information

no activity with 10-hydroxyoctadecanoic acid

729334

Macrococcus caseolyticus

?

-

additional information

no activity with 10-hydroxyoctadecanoic acid

729334

Macrococcus caseolyticus JCSC5402

?

-

myristoleic acid

-

729334

Macrococcus caseolyticus

10-hydroxytetradecanoic acid + H2O

-

?

oleic acid + H2O

-

729334

Macrococcus caseolyticus

10-hydroxyoctadecanoic acid

-

ir

oleic acid + H2O

-

729334

Macrococcus caseolyticus JCSC5402

10-hydroxyoctadecanoic acid

-

ir

palmitoleic acid

-

729334

Macrococcus caseolyticus

10-hydroxyhexadecanoic acid + H2O

-

?

homodimer

2 * 68000, SDS-PAGE; 2 * 68100, calculated from amino acid sequence

Macrococcus caseolyticus

25

-

Macrococcus caseolyticus

15

35

40% activity at 15°C, 75% activity at 20°C, 100% activity at 25°C, 78% activity at 30°C, and 65% activity at 35°C

Macrococcus caseolyticus

25

35

after 120 min, the enzyme activity is nearly unchanges at 25°C, but is reduced to only 25% at 35 °C

Macrococcus caseolyticus

0.03

-

myristoleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

0.13

-

alpha-linolenic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

0.13

-

linoleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

2.67

-

gamma-linolenic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

3.83

-

palmitoleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

7.83

-

oleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

6.5

-

Macrococcus caseolyticus

5.5

7.5

48% activity at pH 5.5, 68% activity at pH 6.0, 100% activity at pH 6.5, 40% activity at pH 7.0, and 20% activity at pH 7.5

Macrococcus caseolyticus

0.13

-

myristoleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

0.33

-

linoleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

0.33

-

oleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

0.42

-

alpha-linolenic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

4.5

-

gamma-linolenic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

22.67

-

palmitoleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

0.13

-

myristoleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

0.33

-

linoleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

0.33

-

oleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

0.42

-

alpha-linolenic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

4.5

-

gamma-linolenic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

22.67

-

palmitoleic acid

at pH 6.5 and 25°C

Macrococcus caseolyticus

747558

Lorenzen

-

Rhodococcus erythropolis olea ...

Rhodococcus erythropolis, Rhodococcus erythropolis DN1

ChemCatChem

10

407-414

2018

-

1

2

-

1

-

1

-

2

-

1

-

5

-

11

1

-

1

-

1

-

1

2

-

1

-

1

-

1

-

5

-

11

1

-

1

-

747692

Fibinger

-

A directed mutational approac ...

Lactobacillus acidophilus

Eur. J. Lipid Sci. Technol.

118

841-848

2016

-

1

-

1

-

1

-

1

-

1

-

1

-

748416

Schmid

-

Biocatalytic study of novel o ...

Chryseobacterium gleum, Chryseobacterium gleum ATCC 35910, Desulfomicrobium baculatum, Desulfomicrobium baculatum DSM 4028, Elizabethkingia meningoseptica, Gemella morbillorum, Gemella morbillorum M424, Haloterrigena thermotolerans, Haloterrigena thermotolerans DSM 11522, Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396, Lactobacillus johnsonii, Mucilaginibacter paludis, Mucilaginibacter paludis DSM 18603, Thermoanaerobacterium thermosaccharolyticum, Thermoanaerobacterium thermosaccharolyticum DSM 571

J. Mol. Catal. B

133

S243-S249

2016

-

9

-

16

-

44

-

9

-

44

-

747562

Hiseni

-

New cofactor-independent hydr ...

Bifidobacterium animalis subsp. lactis, Bifidobacterium breve, Elizabethkingia meningoseptica, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus rhamnosus, Lysinibacillus fusiformis, Macrococcus caseolyticus, Stenotrophomonas maltophilia, Streptococcus pyogenes

ChemCatChem

7

29-37

2015

-

10

-

10

-

10

-

33

4

-

10

-

10

-

10

-

33

4

-

748410

Todea

-

Increase of stability of olea ...

Elizabethkingia meningoseptica

J. Mol. Catal. B

119

40-47

2015

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

729457

Hiseni

Enzymatic hydration activity a ...

Elizabethkingia meningoseptica

Biotechnol. J.

9

814-821

2014

-

1

-

2

-

1

-

1

-

1

-

729071

Seo

Production of a novel compound ...

Lysinibacillus fusiformis

Appl. Microbiol. Biotechnol.

97

8987-8995

2013

-

1

-

1

-

1

2

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

729466

Oh

Production of 10-hydroxy-12,15 ...

Stenotrophomonas maltophilia

Biotechnol. Lett.

35

1487-1493

2013

3

-

1

-

3

-

1

-

1

-

1

-

3

-

1

-

1

-

1

-

1

-

729060

Kim

Production of 10-hydroxysteari ...

Lysinibacillus fusiformis, Lysinibacillus fusiformis KCTC 3454

Appl. Microbiol. Biotechnol.

95

929-937

2012

1

-

1

-

1

4

-

3

2

-

3

-

1

-

1

6

-

11

1

-

4

1

-

1

-

1

-

1

-

4

-

3

2

-

1

-

1

6

-

11

1

-

4

1

-

4

729334

Joo

Biochemical characterization a ...

Macrococcus caseolyticus, Macrococcus caseolyticus JCSC5402

Biochimie

94

907-915

2012

1

-

1

-

5

-

10

6

-

3

2

-

4

-

1

-

1

-

12

1

6

1

-

2

-

1

-

1

2

-

5

-

10

-

6

-

3

2

-

1

-

1

-

12

1

6

1

-

6

730082

Joo

Production of 10-hydroxysteari ...

Stenotrophomonas maltophilia, Stenotrophomonas maltophilia KCTC 1773

J. Biotechnol.

158

17-23

2012

1

-

1

-

1

-

2

-

4

-

1

-

6

-

12

1

-

1

-

1

-

1

-

1

-

1

-

2

-

1

-

6

-

12

1

-

1

-

1

730845

Jeon

-

Bioprocess engineering to prod ...

Stenotrophomonas maltophilia

Process Biochem.

47

941-947

2012

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

704302

Bevers

Oleate hydratase catalyzes the ...

Elizabethkingia meningoseptica

J. Bacteriol.

191

5010-5012

2009

1

-

1

-

5

-

1

2

-

3

-

1

-

6

-

1

-

1

-

1

-

1

-

5

-

1

2

-

1

-

6

-

1

-

1

-

1

-

1

-

5783

Kisic

Oleate hydratase: Studies of s ...

Pseudomonas sp.

Lipids

6

541-545

1971

-

1

-

1

-

6

-

1

-

6

-

5784

Niehaus

Stereospecific hydratation of ...

Pseudomonas sp.

J. Biol. Chem.

245

3790-3797

1970

-

1

-

1

-

3

-

1

-

1

-

3

-

1

-

1

-

5785

Davis

Microbial hydration of cis-9-a ...

Pseudomonas sp.

Lipids

4

356-362

1969

-

1

-

1

-

1

-

Biochemical characterization and FAD-binding analysis of oleate hydratase from Macrococcus caseolyticus

Data extracted from this reference: