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show all sequences of 4.2.1.53

Biochemical characterization and FAD-binding analysis of oleate hydratase from Macrococcus caseolyticus

Joo, Y.C.; Jeong, K.W.; Yeom, S.J.; Kim, Y.S.; Kim, Y.; Oh, D.K.; Biochimie 94, 907-915 (2012)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ethanol
maximum activity in the presence of 2% (v/v) ethanol
Macrococcus caseolyticus
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli ER2566 cells
Macrococcus caseolyticus
Engineering
Amino acid exchange
Commentary
Organism
E50A
the mutant shows reduced activity and FAD binding compared to the wild type enzyme
Macrococcus caseolyticus
E56A
inactive
Macrococcus caseolyticus
G29A
inactive
Macrococcus caseolyticus
G31A
inactive
Macrococcus caseolyticus
S34A
the mutant shows reduced activity and FAD binding compared to the wild type enzyme
Macrococcus caseolyticus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ba2+
-
Macrococcus caseolyticus
Ca2+
-
Macrococcus caseolyticus
Co2+
-
Macrococcus caseolyticus
Cu2+
-
Macrococcus caseolyticus
Fe2+
-
Macrococcus caseolyticus
Mg2+
-
Macrococcus caseolyticus
Mn2+
-
Macrococcus caseolyticus
additional information
not inhibited by EDTA
Macrococcus caseolyticus
Ni2+
-
Macrococcus caseolyticus
Zn2+
-
Macrococcus caseolyticus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.058
-
linoleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
0.059
-
alpha-linolenic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
0.3
-
myristoleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
0.34
-
oleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
0.57
-
palmitoleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
1.12
-
gamma-linolenic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
2 * 68000, SDS-PAGE
Macrococcus caseolyticus
68100
-
2 * 68100, calculated from amino acid sequence
Macrococcus caseolyticus
136000
-
gel filtration
Macrococcus caseolyticus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
oleic acid + H2O
Macrococcus caseolyticus
-
10-hydroxyoctadecanoic acid
-
-
ir
oleic acid + H2O
Macrococcus caseolyticus JCSC5402
-
10-hydroxyoctadecanoic acid
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Macrococcus caseolyticus
B9E972
-
-
Macrococcus caseolyticus JCSC5402
B9E972
-
-
Purification (Commentary)
Commentary
Organism
His-Trap column chromatography
Macrococcus caseolyticus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.3
-
at pH 6.5 and 25C
Macrococcus caseolyticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-linolenic acid
-
729334
Macrococcus caseolyticus
10-hydroxy-12(Z),15(Z)-octadecadienoic acid + 10,13-dihydroxy-15(Z)-octadecenoic acid
-
-
-
-
alpha-linolenic acid
-
729334
Macrococcus caseolyticus JCSC5402
10-hydroxy-12(Z),15(Z)-octadecadienoic acid + 10,13-dihydroxy-15(Z)-octadecenoic acid
-
-
-
-
gamma-linolenic acid
-
729334
Macrococcus caseolyticus
10-hydroxy-6(Z),12(Z)-octadecadienoic acid + 10,13-dihydroxy-6(Z)-octadecenoic acid
-
-
-
-
gamma-linolenic acid
-
729334
Macrococcus caseolyticus JCSC5402
10-hydroxy-6(Z),12(Z)-octadecadienoic acid + 10,13-dihydroxy-6(Z)-octadecenoic acid
-
-
-
-
linoleic acid
-
729334
Macrococcus caseolyticus
10-hydroxy-12(Z)-octadecenoic acid + 10,13-dihydroxyoctadecanoic acid
-
-
-
?
linoleic acid
-
729334
Macrococcus caseolyticus JCSC5402
10-hydroxy-12(Z)-octadecenoic acid + 10,13-dihydroxyoctadecanoic acid
-
-
-
?
additional information
no activity with 10-hydroxyoctadecanoic acid
729334
Macrococcus caseolyticus
?
-
-
-
-
additional information
no activity with 10-hydroxyoctadecanoic acid
729334
Macrococcus caseolyticus JCSC5402
?
-
-
-
-
myristoleic acid
-
729334
Macrococcus caseolyticus
10-hydroxytetradecanoic acid + H2O
-
-
-
?
oleic acid + H2O
-
729334
Macrococcus caseolyticus
10-hydroxyoctadecanoic acid
-
-
-
ir
oleic acid + H2O
-
729334
Macrococcus caseolyticus JCSC5402
10-hydroxyoctadecanoic acid
-
-
-
ir
palmitoleic acid
-
729334
Macrococcus caseolyticus
10-hydroxyhexadecanoic acid + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 68000, SDS-PAGE; 2 * 68100, calculated from amino acid sequence
Macrococcus caseolyticus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Macrococcus caseolyticus
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
15
35
40% activity at 15C, 75% activity at 20C, 100% activity at 25C, 78% activity at 30C, and 65% activity at 35C
Macrococcus caseolyticus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
25
35
after 120 min, the enzyme activity is nearly unchanges at 25C, but is reduced to only 25% at 35 C
Macrococcus caseolyticus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.03
-
myristoleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
0.13
-
alpha-linolenic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
0.13
-
linoleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
2.67
-
gamma-linolenic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
3.83
-
palmitoleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
7.83
-
oleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Macrococcus caseolyticus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
7.5
48% activity at pH 5.5, 68% activity at pH 6.0, 100% activity at pH 6.5, 40% activity at pH 7.0, and 20% activity at pH 7.5
Macrococcus caseolyticus
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
required for activity, maximum activity in the presence of 0.2 M FAD
Macrococcus caseolyticus
additional information
the addition of the cofactor FMN, NAD+, or NADP+ does not affect enzyme activity
Macrococcus caseolyticus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ethanol
maximum activity in the presence of 2% (v/v) ethanol
Macrococcus caseolyticus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli ER2566 cells
Macrococcus caseolyticus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
required for activity, maximum activity in the presence of 0.2 M FAD
Macrococcus caseolyticus
additional information
the addition of the cofactor FMN, NAD+, or NADP+ does not affect enzyme activity
Macrococcus caseolyticus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E50A
the mutant shows reduced activity and FAD binding compared to the wild type enzyme
Macrococcus caseolyticus
E56A
inactive
Macrococcus caseolyticus
G29A
inactive
Macrococcus caseolyticus
G31A
inactive
Macrococcus caseolyticus
S34A
the mutant shows reduced activity and FAD binding compared to the wild type enzyme
Macrococcus caseolyticus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ba2+
-
Macrococcus caseolyticus
Ca2+
-
Macrococcus caseolyticus
Co2+
-
Macrococcus caseolyticus
Cu2+
-
Macrococcus caseolyticus
Fe2+
-
Macrococcus caseolyticus
Mg2+
-
Macrococcus caseolyticus
Mn2+
-
Macrococcus caseolyticus
additional information
not inhibited by EDTA
Macrococcus caseolyticus
Ni2+
-
Macrococcus caseolyticus
Zn2+
-
Macrococcus caseolyticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.058
-
linoleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
0.059
-
alpha-linolenic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
0.3
-
myristoleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
0.34
-
oleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
0.57
-
palmitoleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
1.12
-
gamma-linolenic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
2 * 68000, SDS-PAGE
Macrococcus caseolyticus
68100
-
2 * 68100, calculated from amino acid sequence
Macrococcus caseolyticus
136000
-
gel filtration
Macrococcus caseolyticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
oleic acid + H2O
Macrococcus caseolyticus
-
10-hydroxyoctadecanoic acid
-
-
ir
oleic acid + H2O
Macrococcus caseolyticus JCSC5402
-
10-hydroxyoctadecanoic acid
-
-
ir
Purification (Commentary) (protein specific)
Commentary
Organism
His-Trap column chromatography
Macrococcus caseolyticus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.3
-
at pH 6.5 and 25C
Macrococcus caseolyticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-linolenic acid
-
729334
Macrococcus caseolyticus
10-hydroxy-12(Z),15(Z)-octadecadienoic acid + 10,13-dihydroxy-15(Z)-octadecenoic acid
-
-
-
-
alpha-linolenic acid
-
729334
Macrococcus caseolyticus JCSC5402
10-hydroxy-12(Z),15(Z)-octadecadienoic acid + 10,13-dihydroxy-15(Z)-octadecenoic acid
-
-
-
-
gamma-linolenic acid
-
729334
Macrococcus caseolyticus
10-hydroxy-6(Z),12(Z)-octadecadienoic acid + 10,13-dihydroxy-6(Z)-octadecenoic acid
-
-
-
-
gamma-linolenic acid
-
729334
Macrococcus caseolyticus JCSC5402
10-hydroxy-6(Z),12(Z)-octadecadienoic acid + 10,13-dihydroxy-6(Z)-octadecenoic acid
-
-
-
-
linoleic acid
-
729334
Macrococcus caseolyticus
10-hydroxy-12(Z)-octadecenoic acid + 10,13-dihydroxyoctadecanoic acid
-
-
-
?
linoleic acid
-
729334
Macrococcus caseolyticus JCSC5402
10-hydroxy-12(Z)-octadecenoic acid + 10,13-dihydroxyoctadecanoic acid
-
-
-
?
additional information
no activity with 10-hydroxyoctadecanoic acid
729334
Macrococcus caseolyticus
?
-
-
-
-
additional information
no activity with 10-hydroxyoctadecanoic acid
729334
Macrococcus caseolyticus JCSC5402
?
-
-
-
-
myristoleic acid
-
729334
Macrococcus caseolyticus
10-hydroxytetradecanoic acid + H2O
-
-
-
?
oleic acid + H2O
-
729334
Macrococcus caseolyticus
10-hydroxyoctadecanoic acid
-
-
-
ir
oleic acid + H2O
-
729334
Macrococcus caseolyticus JCSC5402
10-hydroxyoctadecanoic acid
-
-
-
ir
palmitoleic acid
-
729334
Macrococcus caseolyticus
10-hydroxyhexadecanoic acid + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 68000, SDS-PAGE; 2 * 68100, calculated from amino acid sequence
Macrococcus caseolyticus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Macrococcus caseolyticus
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
15
35
40% activity at 15C, 75% activity at 20C, 100% activity at 25C, 78% activity at 30C, and 65% activity at 35C
Macrococcus caseolyticus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
25
35
after 120 min, the enzyme activity is nearly unchanges at 25C, but is reduced to only 25% at 35 C
Macrococcus caseolyticus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.03
-
myristoleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
0.13
-
alpha-linolenic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
0.13
-
linoleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
2.67
-
gamma-linolenic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
3.83
-
palmitoleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
7.83
-
oleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Macrococcus caseolyticus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
7.5
48% activity at pH 5.5, 68% activity at pH 6.0, 100% activity at pH 6.5, 40% activity at pH 7.0, and 20% activity at pH 7.5
Macrococcus caseolyticus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.13
-
myristoleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
0.33
-
linoleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
0.33
-
oleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
0.42
-
alpha-linolenic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
4.5
-
gamma-linolenic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
22.67
-
palmitoleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.13
-
myristoleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
0.33
-
linoleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
0.33
-
oleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
0.42
-
alpha-linolenic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
4.5
-
gamma-linolenic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
22.67
-
palmitoleic acid
at pH 6.5 and 25C
Macrococcus caseolyticus
Other publictions for EC 4.2.1.53
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747558
Lorenzen
-
Rhodococcus erythropolis olea ...
Rhodococcus erythropolis, Rhodococcus erythropolis DN1
ChemCatChem
10
407-414
2018
-
-
1
1
2
-
-
1
-
1
-
-
-
2
-
-
1
-
-
-
5
-
11
1
1
1
-
1
1
1
-
1
-
-
-
-
-
1
1
1
2
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
5
-
11
1
1
1
-
1
1
1
-
-
-
-
-
-
-
-
747692
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Eur. J. Lipid Sci. Technol.
118
841-848
2016
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-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
748416
Schmid
-
Biocatalytic study of novel o ...
Chryseobacterium gleum, Chryseobacterium gleum ATCC 35910, Desulfomicrobium baculatum, Desulfomicrobium baculatum DSM 4028, Elizabethkingia meningoseptica, Gemella morbillorum, Gemella morbillorum M424, Haloterrigena thermotolerans, Haloterrigena thermotolerans DSM 11522, Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396, Lactobacillus johnsonii, Mucilaginibacter paludis, Mucilaginibacter paludis DSM 18603, Thermoanaerobacterium thermosaccharolyticum, Thermoanaerobacterium thermosaccharolyticum DSM 571
J. Mol. Catal. B
133
S243-S249
2016
-
-
9
-
-
-
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
-
44
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
44
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747562
Hiseni
-
New cofactor-independent hydr ...
Bifidobacterium animalis subsp. lactis, Bifidobacterium breve, Elizabethkingia meningoseptica, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus rhamnosus, Lysinibacillus fusiformis, Macrococcus caseolyticus, Stenotrophomonas maltophilia, Streptococcus pyogenes
ChemCatChem
7
29-37
2015
-
10
10
-
-
-
-
-
-
-
10
-
-
10
-
-
-
-
-
-
-
-
33
4
-
-
-
-
-
-
-
10
-
-
-
-
10
10
10
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
33
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
748410
Todea
-
Increase of stability of olea ...
Elizabethkingia meningoseptica
J. Mol. Catal. B
119
40-47
2015
-
-
1
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
729457
Hiseni
Enzymatic hydration activity a ...
Elizabethkingia meningoseptica
Biotechnol. J.
9
814-821
2014
-
-
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