Data extracted from this reference:
Activating Compound
ethanol
maximum activity in the presence of 2% (v/v) ethanol
Macrococcus caseolyticus
Cloned(Commentary)
expressed in Escherichia coli ER2566 cells
Macrococcus caseolyticus
Engineering
E50A
the mutant shows reduced activity and FAD binding compared to the wild type enzyme
Macrococcus caseolyticus
E56A
inactive
Macrococcus caseolyticus
G29A
inactive
Macrococcus caseolyticus
G31A
inactive
Macrococcus caseolyticus
S34A
the mutant shows reduced activity and FAD binding compared to the wild type enzyme
Macrococcus caseolyticus
Inhibitors
Ba2+
Macrococcus caseolyticus
Ca2+
Macrococcus caseolyticus
Co2+
Macrococcus caseolyticus
Cu2+
Macrococcus caseolyticus
Fe2+
Macrococcus caseolyticus
Mg2+
Macrococcus caseolyticus
Mn2+
Macrococcus caseolyticus
additional information
not inhibited by EDTA
Macrococcus caseolyticus
Ni2+
Macrococcus caseolyticus
Zn2+
Macrococcus caseolyticus
KM Value [mM]
0.058
linoleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
0.059
alpha-linolenic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
0.3
myristoleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
0.34
oleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
0.57
palmitoleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
1.12
gamma-linolenic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
Molecular Weight [Da]
68000
2 * 68000, SDS-PAGE
Macrococcus caseolyticus
68100
2 * 68100, calculated from amino acid sequence
Macrococcus caseolyticus
136000
gel filtration
Macrococcus caseolyticus
Natural Substrates/ Products (Substrates)
oleic acid + H2O
Macrococcus caseolyticus
10-hydroxyoctadecanoic acid
ir
oleic acid + H2O
Macrococcus caseolyticus JCSC5402
10-hydroxyoctadecanoic acid
ir
Organism
Macrococcus caseolyticus
B9E972
Macrococcus caseolyticus JCSC5402
B9E972
Purification (Commentary)
His-Trap column chromatography
Macrococcus caseolyticus
Specific Activity [micromol/min/mg]
3.3
at pH 6.5 and 25°C
Macrococcus caseolyticus
Substrates and Products (Substrate)
alpha-linolenic acid
729334
Macrococcus caseolyticus
10-hydroxy-12(Z),15(Z)-octadecadienoic acid + 10,13-dihydroxy-15(Z)-octadecenoic acid
alpha-linolenic acid
729334
Macrococcus caseolyticus JCSC5402
10-hydroxy-12(Z),15(Z)-octadecadienoic acid + 10,13-dihydroxy-15(Z)-octadecenoic acid
gamma-linolenic acid
729334
Macrococcus caseolyticus
10-hydroxy-6(Z),12(Z)-octadecadienoic acid + 10,13-dihydroxy-6(Z)-octadecenoic acid
gamma-linolenic acid
729334
Macrococcus caseolyticus JCSC5402
10-hydroxy-6(Z),12(Z)-octadecadienoic acid + 10,13-dihydroxy-6(Z)-octadecenoic acid
linoleic acid
729334
Macrococcus caseolyticus
10-hydroxy-12(Z)-octadecenoic acid + 10,13-dihydroxyoctadecanoic acid
?
linoleic acid
729334
Macrococcus caseolyticus JCSC5402
10-hydroxy-12(Z)-octadecenoic acid + 10,13-dihydroxyoctadecanoic acid
?
additional information
no activity with 10-hydroxyoctadecanoic acid
729334
Macrococcus caseolyticus
?
additional information
no activity with 10-hydroxyoctadecanoic acid
729334
Macrococcus caseolyticus JCSC5402
?
myristoleic acid
729334
Macrococcus caseolyticus
10-hydroxytetradecanoic acid + H2O
?
oleic acid + H2O
729334
Macrococcus caseolyticus
10-hydroxyoctadecanoic acid
ir
oleic acid + H2O
729334
Macrococcus caseolyticus JCSC5402
10-hydroxyoctadecanoic acid
ir
palmitoleic acid
729334
Macrococcus caseolyticus
10-hydroxyhexadecanoic acid + H2O
?
Subunits
homodimer
2 * 68000, SDS-PAGE; 2 * 68100, calculated from amino acid sequence
Macrococcus caseolyticus
Temperature Optimum [°C]
25
Macrococcus caseolyticus
Temperature Range [°C]
15
35
40% activity at 15°C, 75% activity at 20°C, 100% activity at 25°C, 78% activity at 30°C, and 65% activity at 35°C
Macrococcus caseolyticus
Temperature Stability [°C]
25
35
after 120 min, the enzyme activity is nearly unchanges at 25°C, but is reduced to only 25% at 35 °C
Macrococcus caseolyticus
Turnover Number [1/s]
0.03
myristoleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
0.13
alpha-linolenic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
0.13
linoleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
2.67
gamma-linolenic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
3.83
palmitoleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
7.83
oleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
pH Optimum
6.5
Macrococcus caseolyticus
pH Range
5.5
7.5
48% activity at pH 5.5, 68% activity at pH 6.0, 100% activity at pH 6.5, 40% activity at pH 7.0, and 20% activity at pH 7.5
Macrococcus caseolyticus
Cofactor
FAD
required for activity, maximum activity in the presence of 0.2 M FAD
Macrococcus caseolyticus
additional information
the addition of the cofactor FMN, NAD+, or NADP+ does not affect enzyme activity
Macrococcus caseolyticus
Activating Compound (protein specific)
ethanol
maximum activity in the presence of 2% (v/v) ethanol
Macrococcus caseolyticus
Cloned(Commentary) (protein specific)
expressed in Escherichia coli ER2566 cells
Macrococcus caseolyticus
Cofactor (protein specific)
FAD
required for activity, maximum activity in the presence of 0.2 M FAD
Macrococcus caseolyticus
additional information
the addition of the cofactor FMN, NAD+, or NADP+ does not affect enzyme activity
Macrococcus caseolyticus
Engineering (protein specific)
E50A
the mutant shows reduced activity and FAD binding compared to the wild type enzyme
Macrococcus caseolyticus
E56A
inactive
Macrococcus caseolyticus
G29A
inactive
Macrococcus caseolyticus
G31A
inactive
Macrococcus caseolyticus
S34A
the mutant shows reduced activity and FAD binding compared to the wild type enzyme
Macrococcus caseolyticus
Inhibitors (protein specific)
Ba2+
Macrococcus caseolyticus
Ca2+
Macrococcus caseolyticus
Co2+
Macrococcus caseolyticus
Cu2+
Macrococcus caseolyticus
Fe2+
Macrococcus caseolyticus
Mg2+
Macrococcus caseolyticus
Mn2+
Macrococcus caseolyticus
additional information
not inhibited by EDTA
Macrococcus caseolyticus
Ni2+
Macrococcus caseolyticus
Zn2+
Macrococcus caseolyticus
KM Value [mM] (protein specific)
0.058
linoleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
0.059
alpha-linolenic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
0.3
myristoleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
0.34
oleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
0.57
palmitoleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
1.12
gamma-linolenic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
Molecular Weight [Da] (protein specific)
68000
2 * 68000, SDS-PAGE
Macrococcus caseolyticus
68100
2 * 68100, calculated from amino acid sequence
Macrococcus caseolyticus
136000
gel filtration
Macrococcus caseolyticus
Natural Substrates/ Products (Substrates) (protein specific)
oleic acid + H2O
Macrococcus caseolyticus
10-hydroxyoctadecanoic acid
ir
oleic acid + H2O
Macrococcus caseolyticus JCSC5402
10-hydroxyoctadecanoic acid
ir
Purification (Commentary) (protein specific)
His-Trap column chromatography
Macrococcus caseolyticus
Specific Activity [micromol/min/mg] (protein specific)
3.3
at pH 6.5 and 25°C
Macrococcus caseolyticus
Substrates and Products (Substrate) (protein specific)
alpha-linolenic acid
729334
Macrococcus caseolyticus
10-hydroxy-12(Z),15(Z)-octadecadienoic acid + 10,13-dihydroxy-15(Z)-octadecenoic acid
alpha-linolenic acid
729334
Macrococcus caseolyticus JCSC5402
10-hydroxy-12(Z),15(Z)-octadecadienoic acid + 10,13-dihydroxy-15(Z)-octadecenoic acid
gamma-linolenic acid
729334
Macrococcus caseolyticus
10-hydroxy-6(Z),12(Z)-octadecadienoic acid + 10,13-dihydroxy-6(Z)-octadecenoic acid
gamma-linolenic acid
729334
Macrococcus caseolyticus JCSC5402
10-hydroxy-6(Z),12(Z)-octadecadienoic acid + 10,13-dihydroxy-6(Z)-octadecenoic acid
linoleic acid
729334
Macrococcus caseolyticus
10-hydroxy-12(Z)-octadecenoic acid + 10,13-dihydroxyoctadecanoic acid
?
linoleic acid
729334
Macrococcus caseolyticus JCSC5402
10-hydroxy-12(Z)-octadecenoic acid + 10,13-dihydroxyoctadecanoic acid
?
additional information
no activity with 10-hydroxyoctadecanoic acid
729334
Macrococcus caseolyticus
?
additional information
no activity with 10-hydroxyoctadecanoic acid
729334
Macrococcus caseolyticus JCSC5402
?
myristoleic acid
729334
Macrococcus caseolyticus
10-hydroxytetradecanoic acid + H2O
?
oleic acid + H2O
729334
Macrococcus caseolyticus
10-hydroxyoctadecanoic acid
ir
oleic acid + H2O
729334
Macrococcus caseolyticus JCSC5402
10-hydroxyoctadecanoic acid
ir
palmitoleic acid
729334
Macrococcus caseolyticus
10-hydroxyhexadecanoic acid + H2O
?
Subunits (protein specific)
homodimer
2 * 68000, SDS-PAGE; 2 * 68100, calculated from amino acid sequence
Macrococcus caseolyticus
Temperature Optimum [°C] (protein specific)
25
Macrococcus caseolyticus
Temperature Range [°C] (protein specific)
15
35
40% activity at 15°C, 75% activity at 20°C, 100% activity at 25°C, 78% activity at 30°C, and 65% activity at 35°C
Macrococcus caseolyticus
Temperature Stability [°C] (protein specific)
25
35
after 120 min, the enzyme activity is nearly unchanges at 25°C, but is reduced to only 25% at 35 °C
Macrococcus caseolyticus
Turnover Number [1/s] (protein specific)
0.03
myristoleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
0.13
alpha-linolenic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
0.13
linoleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
2.67
gamma-linolenic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
3.83
palmitoleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
7.83
oleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
pH Optimum (protein specific)
6.5
Macrococcus caseolyticus
pH Range (protein specific)
5.5
7.5
48% activity at pH 5.5, 68% activity at pH 6.0, 100% activity at pH 6.5, 40% activity at pH 7.0, and 20% activity at pH 7.5
Macrococcus caseolyticus
KCat/KM [mM/s]
0.13
myristoleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
0.33
linoleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
0.33
oleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
0.42
alpha-linolenic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
4.5
gamma-linolenic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
22.67
palmitoleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
KCat/KM [mM/s] (protein specific)
0.13
myristoleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
0.33
linoleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
0.33
oleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
0.42
alpha-linolenic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
4.5
gamma-linolenic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
22.67
palmitoleic acid
at pH 6.5 and 25°C
Macrococcus caseolyticus
Other publictions for EC 4.2.1.53
747558
Lorenzen
-
Rhodococcus erythropolis olea ...
Rhodococcus erythropolis, Rhodococcus erythropolis DN1
ChemCatChem
10
407-414
2018
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1
1
2
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1
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1
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1
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5
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11
1
1
1
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1
1
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1
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1
1
1
2
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1
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1
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5
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11
1
1
1
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1
1
1
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-
-
-
747692
Fibinger
-
A directed mutational approac ...
Lactobacillus acidophilus
Eur. J. Lipid Sci. Technol.
118
841-848
2016
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1
1
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748416
Schmid
-
Biocatalytic study of novel o ...
Chryseobacterium gleum, Chryseobacterium gleum ATCC 35910, Desulfomicrobium baculatum, Desulfomicrobium baculatum DSM 4028, Elizabethkingia meningoseptica, Gemella morbillorum, Gemella morbillorum M424, Haloterrigena thermotolerans, Haloterrigena thermotolerans DSM 11522, Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396, Lactobacillus johnsonii, Mucilaginibacter paludis, Mucilaginibacter paludis DSM 18603, Thermoanaerobacterium thermosaccharolyticum, Thermoanaerobacterium thermosaccharolyticum DSM 571
J. Mol. Catal. B
133
S243-S249
2016
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9
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16
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44
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9
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44
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747562
Hiseni
-
New cofactor-independent hydr ...
Bifidobacterium animalis subsp. lactis, Bifidobacterium breve, Elizabethkingia meningoseptica, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus rhamnosus, Lysinibacillus fusiformis, Macrococcus caseolyticus, Stenotrophomonas maltophilia, Streptococcus pyogenes
ChemCatChem
7
29-37
2015
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10
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10
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4
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10
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33
4
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748410
Todea
-
Increase of stability of olea ...
Elizabethkingia meningoseptica
J. Mol. Catal. B
119
40-47
2015
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1
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1
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729457
Hiseni
Enzymatic hydration activity a ...
Elizabethkingia meningoseptica
Biotechnol. J.
9
814-821
2014
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1
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729071
Seo
Production of a novel compound ...
Lysinibacillus fusiformis
Appl. Microbiol. Biotechnol.
97
8987-8995
2013
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1
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1
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729466
Oh
Production of 10-hydroxy-12,15 ...
Stenotrophomonas maltophilia
Biotechnol. Lett.
35
1487-1493
2013
3
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1
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729060
Kim
Production of 10-hydroxysteari ...
Lysinibacillus fusiformis, Lysinibacillus fusiformis KCTC 3454
Appl. Microbiol. Biotechnol.
95
929-937
2012
1
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1
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1
4
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3
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3
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1
1
1
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4
1
1
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4
4
729334
Joo
Biochemical characterization a ...
Macrococcus caseolyticus, Macrococcus caseolyticus JCSC5402
Biochimie
94
907-915
2012
1
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1
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5
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10
6
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3
2
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1
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6
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6
6
730082
Joo
Production of 10-hydroxysteari ...
Stenotrophomonas maltophilia, Stenotrophomonas maltophilia KCTC 1773
J. Biotechnol.
158
17-23
2012
1
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1
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2
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1
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1
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1
1
730845
Jeon
-
Bioprocess engineering to prod ...
Stenotrophomonas maltophilia
Process Biochem.
47
941-947
2012
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704302
Bevers
Oleate hydratase catalyzes the ...
Elizabethkingia meningoseptica
J. Bacteriol.
191
5010-5012
2009
1
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1
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1
2
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5783
Kisic
Oleate hydratase: Studies of s ...
Pseudomonas sp.
Lipids
6
541-545
1971
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6
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1
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6
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-
5784
Niehaus
Stereospecific hydratation of ...
Pseudomonas sp.
J. Biol. Chem.
245
3790-3797
1970
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1
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-
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1
-
-
-
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3
-
1
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-
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1
-
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-
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-
-
-
3
-
1
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1
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5785
Davis
Microbial hydration of cis-9-a ...
Pseudomonas sp.
Lipids
4
356-362
1969
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1
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1
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