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Literature summary for 4.2.1.51 extracted from
- A monofunctional and thermostable prephenate dehydratase from the archaeon Methanocaldococcus jannaschii (2006), Biochemistry, 45, 14101-14110.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| pheA gene is cloned and expressed in Escherichia coli | Methanocaldococcus jannaschii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-Phe | feedback-inhibition | Methanocaldococcus jannaschii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
prephenate | 30°C | Methanocaldococcus jannaschii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31413 | - |
x * 31413, ESI mass spectrometry | Methanocaldococcus jannaschii |
| 31415 | - |
x * 31415, calculated from sequence | Methanocaldococcus jannaschii |
| 45600 | - |
gel filtration | Methanocaldococcus jannaschii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| prephenate | - |
Methanocaldococcus jannaschii | phenylpyruvate + H2O + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31413, ESI mass spectrometry | Methanocaldococcus jannaschii |
| ? | x * 31415, calculated from sequence | Methanocaldococcus jannaschii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MjPDT | - |
Methanocaldococcus jannaschii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 94 | - |
MjPDT unfolds in a two-state manner, Tm: 94°C | Methanocaldococcus jannaschii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12.3 | - |
prephenate | 30°C | Methanocaldococcus jannaschii | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
L-Phe | - |
Methanocaldococcus jannaschii | |
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Release 2023.1 (January 2023)
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