Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-leucine | activation | Bacillus subtilis | |
L-methionine | activation | Bacillus subtilis |
General Stability | Organism |
---|---|
octameric form, whose aggregation is caused by activator molecules is stable, while the dimeric form can be stabilized by glycerol | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-phenylalanine | - |
Bacillus subtilis | |
L-tryptophan | - |
Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
1 * 35000, inactive enzyme in absence of activating molecules | Bacillus subtilis |
35000 | - |
2 * 35000, slightly active | Bacillus subtilis |
35000 | - |
8 * 35000, presence of activating molecules | Bacillus subtilis |
55000 | - |
absence of activating molecules, gel filtration | Bacillus subtilis |
210000 | - |
presence of activating molecules, gel filtration | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate | Bacillus subtilis | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.5 | - |
- |
Bacillus subtilis |
Storage Stability | Organism |
---|---|
4°C, 20% glycerol or presence of L-Met, L-Leu, prephenate, stable for several months | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate | - |
Bacillus subtilis | phenylpyruvate + H2O + CO2 | - |
? | |
prephenate | - |
Bacillus subtilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, slightly active | Bacillus subtilis |
monomer | 1 * 35000, inactive enzyme in absence of activating molecules | Bacillus subtilis |
octamer | 8 * 35000, presence of activating molecules | Bacillus subtilis |