BRENDA - Enzyme Database
show all sequences of 4.2.1.50

Purification and characterization of beta-(pyrazol-1-yl)-L-alanine synthase from Citrullus vulgaris

Murakoshi, I.; Ikegami, F.; Hinuma, Y.; Hanma, Y.; Phytochemistry 23, 973-977 (1984)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
sensitive to freezing even in 20% glycerol
Citrullus lanatus subsp. vulgaris
Inhibitors
Inhibitors
Commentary
Organism
Structure
hydroxylamine
50 mM almost complete inhibition
Citrullus lanatus subsp. vulgaris
potassium cyanide
10 mM almost complete inhibition
Citrullus lanatus subsp. vulgaris
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5
-
O-acetyl-L-serine
-
Citrullus lanatus subsp. vulgaris
74
-
pyrazole
-
Citrullus lanatus subsp. vulgaris
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
2 * 32000, SDS-PAGE
Citrullus lanatus subsp. vulgaris
58000
-
gel filtration
Citrullus lanatus subsp. vulgaris
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
O-Acetyl-L-serine + pyrazole
Citrullus lanatus subsp. vulgaris
-
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Citrullus lanatus subsp. vulgaris
-
water melon
-
Purification (Commentary)
Commentary
Organism
-
Citrullus lanatus subsp. vulgaris
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seedling
-
Citrullus lanatus subsp. vulgaris
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.355
-
-
Citrullus lanatus subsp. vulgaris
Storage Stability
Storage Stability
Organism
0C, 1 month
Citrullus lanatus subsp. vulgaris
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no activity with L-serine
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
O-Acetyl-L-serine + 3-amino-1,2,4-triazole
10.6% of activity with pyrazole
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
O-Acetyl-L-serine + N-hydroxyurea
8.7% of activity with pyrazole
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
O-Acetyl-L-serine + pyrazole
-
5779
Citrullus lanatus subsp. vulgaris
3-(Pyrazol-1-yl)-L-alanine + acetate
-
5779
Citrullus lanatus subsp. vulgaris
-
O-Acetyl-L-serine + pyrazole
-
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
O-Succinyl-L-serine + pyrazole
16.4% of activity with O-Acetyl-L-serine
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
O-Sulfo-L-serine + pyrazole
2.5% of activity with O-Acetyl-L-serine
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 32000, SDS-PAGE
Citrullus lanatus subsp. vulgaris
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
7.4
O-acetyl-L-serine + pyrazole
Citrullus lanatus subsp. vulgaris
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
8.5
50% of maximal activity with O-acetyl-L-serine + pyrazole at pH 6.5 and pH 8.5
Citrullus lanatus subsp. vulgaris
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
1 molecule bound to each subunit; maximal activation by 40% at 0.25 mM
Citrullus lanatus subsp. vulgaris
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
1 molecule bound to each subunit; maximal activation by 40% at 0.25 mM
Citrullus lanatus subsp. vulgaris
General Stability (protein specific)
General Stability
Organism
sensitive to freezing even in 20% glycerol
Citrullus lanatus subsp. vulgaris
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
hydroxylamine
50 mM almost complete inhibition
Citrullus lanatus subsp. vulgaris
potassium cyanide
10 mM almost complete inhibition
Citrullus lanatus subsp. vulgaris
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5
-
O-acetyl-L-serine
-
Citrullus lanatus subsp. vulgaris
74
-
pyrazole
-
Citrullus lanatus subsp. vulgaris
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
2 * 32000, SDS-PAGE
Citrullus lanatus subsp. vulgaris
58000
-
gel filtration
Citrullus lanatus subsp. vulgaris
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
O-Acetyl-L-serine + pyrazole
Citrullus lanatus subsp. vulgaris
-
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Citrullus lanatus subsp. vulgaris
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seedling
-
Citrullus lanatus subsp. vulgaris
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.355
-
-
Citrullus lanatus subsp. vulgaris
Storage Stability (protein specific)
Storage Stability
Organism
0C, 1 month
Citrullus lanatus subsp. vulgaris
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no activity with L-serine
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
O-Acetyl-L-serine + 3-amino-1,2,4-triazole
10.6% of activity with pyrazole
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
O-Acetyl-L-serine + N-hydroxyurea
8.7% of activity with pyrazole
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
O-Acetyl-L-serine + pyrazole
-
5779
Citrullus lanatus subsp. vulgaris
3-(Pyrazol-1-yl)-L-alanine + acetate
-
5779
Citrullus lanatus subsp. vulgaris
-
O-Acetyl-L-serine + pyrazole
-
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
O-Succinyl-L-serine + pyrazole
16.4% of activity with O-Acetyl-L-serine
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
O-Sulfo-L-serine + pyrazole
2.5% of activity with O-Acetyl-L-serine
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 32000, SDS-PAGE
Citrullus lanatus subsp. vulgaris
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
7.4
O-acetyl-L-serine + pyrazole
Citrullus lanatus subsp. vulgaris
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
8.5
50% of maximal activity with O-acetyl-L-serine + pyrazole at pH 6.5 and pH 8.5
Citrullus lanatus subsp. vulgaris
Other publictions for EC 4.2.1.50
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
5782
Saito
Production of plant non-protei ...
Citrullus lanatus subsp. vulgaris
Biol. Pharm. Bull.
20
47-53
1997
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5781
Noji
Evidence for identity of beta- ...
Citrullus lanatus subsp. vulgaris
Biochem. Biophys. Res. Commun.
197
1111-1117
1993
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5779
Murakoshi
-
Purification and characterizat ...
Citrullus lanatus subsp. vulgaris
Phytochemistry
23
973-977
1984
-
-
-
-
-
1
2
2
-
-
2
1
-
1
-
-
1
-
-
1
1
1
7
1
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
1
-
2
-
2
-
-
2
1
-
-
-
1
-
1
1
1
7
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
5780
Dunnill
-
The biosynthesis of bety-pyraz ...
Cucumis melo, Cucumis sativus, Cucurbita pepo
J. Exp. Bot.
14
237-248
1963
-
-
-
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
1
-
-
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-
-
-
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-