Literature summary for 4.2.1.48 extracted from

Katane, M.; Ariyoshi, M.; Tateishi, S.; Koiwai, S.; Takaku, K.; Nagai, K.; Nakayama, K.; Saitoh, Y.; Miyamoto, T.; Sekine, M.; Mita, M.; Hamase, K.; Matoba, S.; Homma, H.
Structural and enzymatic properties of mammalian D-glutamate cyclase (2018), Arch. Biochem. Biophys., 654, 10-18 .

Search for more literature for 4.2.1.48

Cloned(Commentary)

Cloned (Comment)	Organism
-	Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	significantly decreases activity with 5-oxo-D-proline and activity with D-glutamate	Mus musculus
ATP	significantly decreases activity with 5-oxo-D-proline and activity with D-glutamate	Mus musculus
EDTA	-	Mus musculus
FAD	-	Mus musculus
FMN	-	Mus musculus
Mn2+	completely abolishes activity with 5-oxo-D-proline, stimulates activity with D-glutamate	Mus musculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
31.9	-	D-glutamate	pH 8.0, 37°C	Mus musculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	highly expressed in the mitochondrial matrix	Mus musculus	5739	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
CaCl2	no significant activity with 5-oxo-D-proline, stimulates activity with D-glutamate	Mus musculus
CuCl2	no significant activity with 5-oxo-D-proline, stimulates activity with D-glutamate	Mus musculus
MgCl2	no significant activity with 5-oxo-D-proline, stimulates activity with D-glutamate	Mus musculus
Mn2+	completely abolishes activity with 5-oxo-D-proline, stimulates activity with D-glutamate	Mus musculus
ZnCl2	no significant activity with 5-oxo-D-proline, stimulates activity with D-glutamate	Mus musculus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
146000	-	non-denaturing PAGE	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glutamate	Mus musculus	the metalloenzyme that degrades D-glutamate in the mitochondrial matrix in mammalian cells	5-oxo-D-proline + H2O	-	r

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	Q8BH86	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Mus musculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
heart	highly expressed in the mitochondrial matrix in the heart	Mus musculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-oxo-D-proline + H2O	-	Mus musculus	D-glutamate	-	r
D-glutamate	-	Mus musculus	5-oxo-D-proline + H2O	-	r
D-glutamate	the metalloenzyme that degrades D-glutamate in the mitochondrial matrix in mammalian cells	Mus musculus	5-oxo-D-proline + H2O	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 65874, calculated from sequence	Mus musculus

Synonyms

Synonyms	Comment	Organism
DGLUCY	-	Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
7.67	-	5-oxo-D-proline	pH 8.0, 37°C	Mus musculus
35.6	-	D-glutamate	pH 8.0, 37°C	Mus musculus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Mus musculus

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	8	small but significant increase in activity occurrs between pH 6.0 to 8.0	Mus musculus

General Information

General Information	Comment	Organism
metabolism	disrupts D-glutamate and/or 5-oxo-D-proline levels, contributing to the onset and/or exacerbation of heart failure	Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1121	-	D-glutamate	pH 8.0, 37°C	Mus musculus

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Release 2023.1 (January 2023)