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Literature summary for 4.2.1.47 extracted from
- Biochemical and functional studies on the Burkholderia cepacia complex bceN gene, encoding a GDP-D-mannose 4,6-dehydratase (2013), PLoS ONE, 8, e56902.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Burkholderia cepacia |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GDP-alpha-D-mannose | substrate inhibition | Burkholderia cepacia |  |
| Mg2+ | high concentrations of MgCl2 (25 mM) have an inhibitory effect on enzyme activity | Burkholderia cepacia | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.024 | - |
GDP-alpha-D-mannose | at pH 7.4 and 25°C, in the presence of 1 mol MgCl2 per mol of protein | Burkholderia cepacia | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity, the enzyme inactive in its absence | Burkholderia cepacia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 162000 | - |
gel filtration | Burkholderia cepacia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-alpha-D-mannose | Burkholderia cepacia | - |
GDP-4-dehydro-alpha-D-rhamnose + H2O | - |
? | |
| GDP-alpha-D-mannose | Burkholderia cepacia J2315 | - |
GDP-4-dehydro-alpha-D-rhamnose + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia cepacia | B4EFL2 | - |
- |
| Burkholderia cepacia J2315 | B4EFL2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Hi-Trap chelating column chromatography | Burkholderia cepacia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-alpha-D-mannose | - |
Burkholderia cepacia | GDP-4-dehydro-alpha-D-rhamnose + H2O | - |
? | |
| GDP-alpha-D-mannose | - |
Burkholderia cepacia J2315 | GDP-4-dehydro-alpha-D-rhamnose + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Burkholderia cepacia |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bceN | - |
Burkholderia cepacia |
| GDP-D-mannose-4,6-dehydratase | - |
Burkholderia cepacia |
| GMD | - |
Burkholderia cepacia |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
GDP-alpha-D-mannose | at pH 7.4 and 25°C, in the presence of 1 mol MgCl2 per mol of protein | Burkholderia cepacia | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | unaffected by NAD+ or NADP+ | Burkholderia cepacia |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.913 | - |
GDP-alpha-D-mannose | at pH 7.4 and 25°C, in the presence of 1 mol MgCl2 per mol of protein | Burkholderia cepacia | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.977 | - |
GDP-alpha-D-mannose | at pH 7.4 and 25°C, in the presence of 1 mol MgCl2 per mol of protein | Burkholderia cepacia | |
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