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Literature summary for 4.2.1.47 extracted from

  • Jang, M.; Lee, W.; Shin, S.; Han, N.; Seo, J.; Kim, M.
    Molecular cloning of the genes for GDP-mannose 4,6-dehydratase and GDP-L-fucose synthetase from Bacteroides thetaiotaomicron (2010), Food Sci. Biotechnol., 19, 849-855.
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Cloned(Commentary)

Cloned (Comment) Organism
GMD sequence determination and analysis, sequence comparison and phylogenetic tree, functional overexpression of soluble GMD in Escherichia coli strain BL21(DE3) from an IPTG-inducible expression vector, optimum expression conditions are 30°C and 0.1 mM IPTG Bacteroides thetaiotaomicron

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacteroides thetaiotaomicron co-expression and activities of GDP-mannose 4,6-dehydratase and GDP-L-fucose synthetase lead toptoduction of GDP-L-fucose ?
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 ?
additional information Bacteroides thetaiotaomicron VPI-5482 co-expression and activities of GDP-mannose 4,6-dehydratase and GDP-L-fucose synthetase lead toptoduction of GDP-L-fucose ?
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 ?

Organism

Organism UniProt Comment Textmining
Bacteroides thetaiotaomicron
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 ATCC 29148D-5
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Bacteroides thetaiotaomicron VPI-5482
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 ATCC 29148D-5
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information co-expression and activities of GDP-mannose 4,6-dehydratase and GDP-L-fucose synthetase lead toptoduction of GDP-L-fucose Bacteroides thetaiotaomicron ?
-
 ?
additional information co-expression and activities of GDP-mannose 4,6-dehydratase and GDP-L-fucose synthetase lead toptoduction of GDP-L-fucose Bacteroides thetaiotaomicron VPI-5482 ?
-
 ?

Synonyms

Synonyms Comment Organism
GDP-mannose 4, 6-dehydratase
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 Bacteroides thetaiotaomicron
GMD
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 Bacteroides thetaiotaomicron

General Information

General Information Comment Organism
evolution GMD belongs to the reductase-epimerase-dehydrogenase superfamily which has a catalytic triad, Ser-Tyr-Lys, that is highly conserved Bacteroides thetaiotaomicron
physiological function GMD is involved in the breakdown of ingested polysaccharides into a form that could not be absorbed and utilized by the host otherwise Bacteroides thetaiotaomicron