Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NAD+ | during RmlB enzyme turnover, NAD+ extracts a hydride from the C40 atom of dTDP-D-glucose and forms NADH. After the elimination of water, the hydride is then returned to the C60 atom of dTDP-4-dehydro-5,6-glucosene intermediate and NADH regenerates NAD+. The optimal concentration of NAD+ is between 0.01 mM and 0.02 mM | Mycobacterium tuberculosis |
Application | Comment | Organism |
---|---|---|
analysis | an absorbance-based microtiter plate assay is developed for RmlB activity. It can be used for high-throughput screening of RmlB inhibitors | Mycobacterium tuberculosis |
pharmacology | the enzyme is essential to mycobacterial growth and is not found in humans, therefore, it is a potential target for developing new anti-tuberculosis drugs | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 0.5 mM, about 50% loss of activity | Mycobacterium tuberculosis | |
dTDP | competitive inhibitor | Mycobacterium tuberculosis | |
dTTP | competitive inhibitor | Mycobacterium tuberculosis | |
K+ | 1 mM, about 10% loss of activity | Mycobacterium tuberculosis | |
Mg2+ | 0.5 mM, about 30% loss of activity | Mycobacterium tuberculosis | |
Mn2+ | 0.2 mM, about 50% loss of activity | Mycobacterium tuberculosis | |
Na+ | 1 mM, about 25% loss of activity | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
dTDP-alpha-D-glucose | pH 7.5, 30°C | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | metal ion is not required for RmlB enzymatic reaction. Stimulated by Mg2+ at the low concentration (0.1 mM) but shows different levels of inhibition by increased Mg2+ concentration | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
SDS-PAGE | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WN65 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WN65 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-alpha-D-glucose | - |
Mycobacterium tuberculosis | dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H2O | - |
? | |
dTDP-alpha-D-glucose | - |
Mycobacterium tuberculosis H37Rv | dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RmlB | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Mycobacterium tuberculosis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | 60 | 25°C: about 55% of maximal activity, 60°C: about 40% of maximal activity | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
466.6 | - |
dTDP-alpha-D-glucose | pH 7.5, 30°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
Hepes buffer | Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | pH 6.5: about 45% of maximal activity, pH 8.5: about 45% of maximal activity, HEPES buffer | Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00267 | - |
dTDP | pH 7.5, 30°C | Mycobacterium tuberculosis | |
0.00567 | - |
dTTP | pH 7.5, 30°C | Mycobacterium tuberculosis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.006 | - |
pH 7.5, 30°C | Mycobacterium tuberculosis | dTDP | |
0.032 | - |
pH 7.5, 30°C | Mycobacterium tuberculosis | dTTP |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in biosynthesis of dTDP-L-rhamnose, which is a sugar donor to the synthesis of the cell wall linker, D-N-acetylglucosamine-L-rhamnose | Mycobacterium tuberculosis |