BRENDA - Enzyme Database show
show all sequences of 4.2.1.46

Concerted and stepwise dehydration mechanisms observed in wild-type and mutated Escherichia coli dTDP-glucose 4,6-dehydratase

Hegeman, A.D.; Gross, J.W.; Frey, P.A.; Biochemistry 41, 2797-2804 (2002)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
D135A
switch from a concerted to stepwise dehydration mechanism is due to the loss of control over the glucosyl C5–C6 bond rotation in the active site
Escherichia coli
D135N
switch from a concerted to stepwise dehydration mechanism is due to the loss of control over the glucosyl C5–C6 bond rotation in the active site
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTDP-glucose
the conversion takes place in three steps: dehydrogenation to dTDP-4-ketoglucose, dehydration to dTDP-4-ketoglucose-5,6-ene and rereduction of C6 to the methyl group
650136
Escherichia coli
dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
650136
Escherichia coli
?
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
tightly bound coenzyme
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
tightly bound coenzyme
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D135A
switch from a concerted to stepwise dehydration mechanism is due to the loss of control over the glucosyl C5–C6 bond rotation in the active site
Escherichia coli
D135N
switch from a concerted to stepwise dehydration mechanism is due to the loss of control over the glucosyl C5–C6 bond rotation in the active site
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTDP-glucose
the conversion takes place in three steps: dehydrogenation to dTDP-4-ketoglucose, dehydration to dTDP-4-ketoglucose-5,6-ene and rereduction of C6 to the methyl group
650136
Escherichia coli
dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
650136
Escherichia coli
?
Other publictions for EC 4.2.1.46
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747917
Li
Characterization of the dTDP- ...
Campylobacter jejuni
Glycobiology
27
358-369
2017
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1
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746731
Shi
A 96-well microtiter plate as ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Anal. Biochem.
498
53-58
2016
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2
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747027
Feng
Identification of a dTDP-rham ...
Caenorhabditis elegans
Biochem. J.
473
1507-1521
2016
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749282
Ma
-
Insights into the catalytic m ...
Streptomyces venezuelae
RSC Adv.
4
35449-35458
2014
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730235
Wu
Identification of elaiophylin ...
Streptomyces sp., Streptomyces sp. 7-145
J. Nat. Prod.
76
2153-2157
2013
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729054
Chen
Distribution of dTDP-glucose-4 ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) 5187, Dietzia sp. 3149, Dietzia sp. DQ12-45-1b 3149, Ensifer sp. AS08, Ensifer sp. AS08 448, Filomicrobium sp. 454, Glycomyces sp. 3338, Micromonospora sp. 114, Micromonospora sp., Micromonospora sp. 282, Micromonospora sp. 3113, Micromonospora sp. 3134, Micromonospora sp. 3137, Micromonospora sp. 3191, Micromonospora sp. 3372-2, Micromonospora sp. 3387, Micromonospora sp. 3437-1, Micromonospora sp. 5297, Nocardia sp., Nocardia sp. 5314, Prauseria sp. 3425, Pseudomonas sp., Pseudomonas sp. 5302, Rhodococcus sp., Rhodococcus sp. 3376-1, Rhodospirillaceae bacterium 5305, Saccharothrix sp. 5133, Shimazuella sp. 3435, Streptomyces sp., Streptomyces sp. 3127, Streptomyces sp. 3419, Streptomyces sp. 5175, Streptomyces sp. 5191, Streptomyces sp. 5311, Streptomyces sp. 5320, Streptomyces sp. 568, Streptomyces sp. SCC 2136, Streptosporangium sp., Streptosporangium sp. 5322, Verrucosispora sp. 3133
Appl. Microbiol. Biotechnol.
90
1347-1359
2011
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41
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701859
Singh
Precursor for biosynthesis of ...
Streptomyces peucetius
Appl. Microbiol. Biotechnol.
85
1565-1574
2010
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1
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1
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1
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2
2
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716065
Parakkottil Chothi
Identification of an L-rhamnos ...
Acanthamoeba polyphaga Mimivirus, Acanthocystis turfacea chlorella virus 1
J. Virol.
84
8829-8838
2010
-
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2
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2
4
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2
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2
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2
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4
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2
2
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2
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2
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690812
Pfoestl
Biosynthesis of dTDP-3-acetami ...
Thermoanaerobacterium thermosaccharolyticum, Thermoanaerobacterium thermosaccharolyticum E207-71
Biochem. J.
410
187-194
2008
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1
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652536
Allard
High resolution X-ray structur ...
Streptomyces venezuelae
J. Biol. Chem.
279
2211-2220
2004
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1
1
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664694
Du
Identification and functional ...
Streptoalloteichus tenebrarius
Curr. Microbiol.
49
99-107
2004
-
1
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-
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6
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1
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650136
Hegeman
Concerted and stepwise dehydra ...
Escherichia coli
Biochemistry
41
2797-2804
2002
-
-
-
-
2
-
-
-
-
-
-
-
-
2
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1
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1
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1
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1
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652770
Sohng
-
Function of lysine-148 in dTDP ...
Streptomyces antibioticus, Streptomyces antibioticus Tue99
J. Microbiol. Biotechnol.
12
217-221
2002
-
-
-
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2
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1
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1
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1
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2
1
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650028
Gross
Dehydration is catalyzed by gl ...
Escherichia coli
Biochemistry
40
12497-12504
2001
-
-
-
-
9
-
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2
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2
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19
-
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1
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1
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9
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2
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19
-
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650072
Hegeman
Probing catalysis by Escherich ...
Escherichia coli
Biochemistry
40
6598-6610
2001
-
-
-
-
17
-
2
19
-
-
-
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-
2
-
-
1
-
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2
-
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-
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20
-
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1
2
-
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1
-
17
-
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2
2
19
-
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1
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-
2
-
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-
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20
-
-
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-
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-
-
650089
Gerratana
Mechanistic roles of Thr134, T ...
Escherichia coli
Biochemistry
40
9187-9195
2001
-
-
-
-
7
-
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8
-
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2
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1
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8
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1
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1
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7
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8
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1
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8
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652851
Allard
The crystal structure of dTDP- ...
Salmonella enterica
J. Mol. Biol.
307
283-295
2001
-
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1
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1
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4
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1
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2
1
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1
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1
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2
1
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649133
Allard
The purification, crystallizat ...
Salmonella enterica
Acta Crystallogr. Sect. D
56
222-225
2000
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1
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1
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649934
Gross
Characterization of enzymatic ...
Escherichia coli
Biochemistry
39
13633-13640
2000
-
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652771
Yoo
-
Expression of orf7(oxi III) as ...
Streptomyces antibioticus, Streptomyces antibioticus Tu99
J. Microbiol. Biotechnol.
9
206-212
1999
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1
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1
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5725
Stein
Combined preparative enzymatic ...
Salmonella enterica subsp. enterica serovar Typhimurium
Glycoconj. J.
15
139-145
1998
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1
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2
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1
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1
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1
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1
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-
5724
Naundorf
Substrate specificity of nativ ...
Escherichia coli
Carbohydr. Res.
285
141-150
1996
-
1
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3
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2
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3
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3
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1
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3
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3
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3
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5723
Linton
Cloning of the genes encoding ...
Saccharopolyspora erythraea
Gene
153
33-40
1995
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1
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1
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1
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5726
Thompson
Purification and characterizat ...
Streptomyces peucetius, Streptomyces sp. C5
J. Gen. Microbiol.
138
779-786
1992
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6
4
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2
2
-
7
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2
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4
2
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2
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2
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2
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6
-
4
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2
2
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2
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2
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4
2
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2
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5722
Romana
High level expression and puri ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biochem. Biophys. Res. Commun.
174
846-852
1991
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1
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1
1
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1
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1
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1
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3
1
1
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1
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1
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1
1
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1
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2
1
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1
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1
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3
1
1
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1
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5712
Vara
Purification of thymidine-diph ...
Saccharopolyspora erythraea
J. Biol. Chem.
263
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1988
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2
2
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1
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2
1
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1
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2
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2
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2
1
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1
1
2
1
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1
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5713
Matern
Stoffwechselprodukte von Mikro ...
Streptomyces rimosus
Arch. Mikrobiol.
88
37-48
1973
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3
1
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1
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1
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1
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1
2
2
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1
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1
1
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1
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3
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1
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1
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1
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1
2
2
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1
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1
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5714
Glaser
-
Dehydration in nucleotide-link ...
Escherichia coli
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
5
465-480
1971
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4
1
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3
1
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1
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2
1
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1
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1
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1
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4
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1
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3
1
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2
1
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1
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5715
Zarkowsky
The mechanism of 6-deoxyhexose ...
Escherichia coli
J. Biol. Chem.
245
6599-6606
1970
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-
-
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-
-
2
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2
1
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1
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2
1
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1
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1
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2
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2
1
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2
1
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5716
Zarkowsky
The mechanism of 6-deoxyhexose ...
Escherichia coli
Biochem. Biophys. Res. Commun.
38
787-793
1970
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-
-
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2
1
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1
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1
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2
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1
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2
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1
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1
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2
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5717
Wang
Biological mechanisms involved ...
Escherichia coli
J. Biol. Chem.
245
8-14
1970
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1
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1
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1
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2
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1
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1
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1
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2
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5718
Zarkowsky
The mechanism of 6-deoxyhexose ...
Escherichia coli
J. Biol. Chem.
244
4750-4756
1969
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-
-
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2
2
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1
1
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1
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1
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1
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3
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1
1
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1
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1
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2
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2
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1
1
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1
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1
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3
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1
1
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5719
Wang
Biological mechanisms involved ...
Escherichia coli
J. Biol. Chem.
244
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1969
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1
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2
1
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1
1
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1
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1
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1
1
2
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1
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1
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1
1
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2
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1
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1
1
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1
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1
1
2
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1
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5720
Melo
The mechanism of 6-deoxyhexose ...
Escherichia coli
J. Biol. Chem.
243
1467-1474
1968
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1
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1
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1
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1
2
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1
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1
2
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5721
Gilbert
Thymidine diphosphate 4-acetam ...
Escherichia coli
J. Biol. Chem.
240
1305-1308
1965
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1
2
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1
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1
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1
2
2
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1
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1
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1
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1
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2
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