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Literature summary for 4.2.1.46 extracted from

  • Thompson, M.W.; Strohl, W.R.; Floss, H.G.
    Purification and characterization of TDP-D-glucose 4,6-dehydratase from anthracycline-producing streptomycetes (1992), J. Gen. Microbiol., 138, 779-786.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
TDP 58% inhibition at 2 mM Streptomyces peucetius
TDP 62% inhibition at 2 mM Streptomyces sp. C5
TMP 23% inhibition at 2 mM Streptomyces peucetius
TMP 25% inhibition at 2 mM Streptomyces sp. C5
TTP 40% inhibition at 2 mM Streptomyces peucetius
TTP 37% inhibition at 2 mM Streptomyces sp. C5

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
NAD+
-
Streptomyces sp. C5
0.02
-
NAD+
-
Streptomyces peucetius
0.031
-
dTDPglucose
-
Streptomyces sp. C5
0.035
-
dTDPglucose
-
Streptomyces peucetius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
2 * 36000, gel filtration, SDS-PAGE Streptomyces peucetius
39000
-
2 * 39000, gel filtration, SDS-PAGE Streptomyces sp. C5

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTDP-glucose Streptomyces peucetius
-
?
-
?
dTDP-glucose Streptomyces sp. C5
-
?
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces peucetius
-
-
-
Streptomyces sp. C5
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces peucetius
-
Streptomyces sp. C5

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
dTDPglucose Streptomyces peucetius
0.31
-
dTDPglucose Streptomyces sp. C5

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-glucose
-
Streptomyces peucetius ?
-
?
dTDP-glucose
-
Streptomyces sp. C5 ?
-
?
additional information not: ADPglucose, ADPmannose, GDPmannose, GDPglucose, UDPgalactose Streptomyces peucetius ?
-
?
additional information not: ADPglucose, ADPmannose, GDPmannose, GDPglucose, UDPgalactose Streptomyces sp. C5 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 36000, gel filtration, SDS-PAGE Streptomyces peucetius
dimer 2 * 39000, gel filtration, SDS-PAGE Streptomyces sp. C5

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
dTDPglucose Streptomyces sp. C5
7.8
-
dTDPglucose Streptomyces peucetius

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Streptomyces peucetius
NAD+
-
Streptomyces sp. C5