Literature summary for 4.2.1.41 extracted from

Taberman, H.; Andberg, M.; Parkkinen, T.; Richard, P.; Hakulinen, N.; Koivula, A.; Rouvinen, J.
Purification, crystallization and preliminary X-ray diffraction analysis of a novel keto-deoxy-D-galactarate (KDG) dehydratase from Agrobacterium tumefaciens (2014), Acta Crystallogr. Sect. F, 70, 49-52 .

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Agrobacterium tumefaciens
expression in Escherichia coli	Agrobacterium fabrum

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystallized by the hanging-drop vapour-diffusion method. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 169.1, b = 117.8, c= 74.3 A, beta = 112.4° and an asymmetric unit of four monomers. X-ray diffraction data are collected to 1.9 A resolution using synchrotron radiation	Agrobacterium tumefaciens
hanging drop vapour-diffusion method	Agrobacterium fabrum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33000	-	SDS-PAGE	Agrobacterium tumefaciens
33000	-	Strep-tagged recombinant enzyme, SDS-PAGE	Agrobacterium fabrum
33526	-	Strep-tagged recombinant enzyme, calculated from sequence	Agrobacterium fabrum

Organism

Organism	UniProt	Comment	Textmining
Agrobacterium fabrum	Q8UB77	-	-
Agrobacterium fabrum ATCC 33970	Q8UB77	-	-
Agrobacterium tumefaciens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Agrobacterium tumefaciens
expressed in Escherichia coli with a C-terminal Strep-tag to facilitate affinity purification of the protein	Agrobacterium fabrum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
15.1	-	pH 7.5, temperature not specified in the publication	Agrobacterium fabrum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-deoxy-2-dehydro-L-threo-hexarate	-	Agrobacterium tumefaciens	2-oxoglutaric semialdehyde + CO2	-	?

Subunits

Subunits	Comment	Organism
?	x * 33000, SDS-PAGE	Agrobacterium tumefaciens

Synonyms

Synonyms	Comment	Organism
KDG dehydratase	-	Agrobacterium tumefaciens
keto-deoxy-D-galactarate dehydratase	-	Agrobacterium tumefaciens

General Information

General Information	Comment	Organism
metabolism	the enzyme is involved in the oxidative pathway of D-galacturonic acid	Agrobacterium tumefaciens

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Release 2023.1 (January 2023)