Literature summary for 4.2.1.40 extracted from

Palmer, D.R.J.; Gerit, J.A.
Evolution of enzymatic activities: multiple pathways for generating and partitioning a common enolic intermediate by glucarate dehydratase from Pseudomonas putida (1996), J. Am. Chem. Soc., 118, 10323-10342.

No PubMed abstract available

Search for more literature for 4.2.1.40

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.067	-	D-glucarate	-	Pseudomonas putida
0.65	-	D-glucarate	-	Pseudomonas putida

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H2O	regiospecific	Pseudomonas putida	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucarate	-	Pseudomonas putida	3-deoxy-L-threo-2-hexulosarate + H2O	i.e. 4-deoxy-5-ketoglucarate	?
D-glucarate	epimerization	Pseudomonas putida	D-idarate	epimerization	r
D-idarate	epimerization	Pseudomonas putida	D-glucarate	epimerization	r
L-idarate	-	Pseudomonas putida	3-deoxy-L-threo-2-hexulosarate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3	-	D-glucarate	-	Pseudomonas putida
4	-	L-idarate	-	Pseudomonas putida

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Release 2023.1 (January 2023)