Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Star cells | Methanocaldococcus jannaschii |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, small subunit, using 60% (w/v) 2-methyl-2,4-pentanediol and 0.1 M bicine pH 9.7 | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | P81291 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap HP column chromatography and Superdex 200 gel filtration | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-homoaconitate | - |
Methanocaldococcus jannaschii | homoisocitrate + H2O | - |
? | |
cis-homoaconitate | - |
Methanocaldococcus jannaschii DSM 2661 | homoisocitrate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | x-ray crystallography | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
Homoaconitase | - |
Methanocaldococcus jannaschii |
leuC | large subunit | Methanocaldococcus jannaschii |
MJ0499 | - |
Methanocaldococcus jannaschii |