Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sulfhydryl compounds | required | Clostridium tetanomorphum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-malate | competitive with L-malate | Clostridium tetanomorphum | |
L-Tartrate | - |
Clostridium tetanomorphum | |
MnCl2 | - |
Clostridium tetanomorphum | |
succinate | - |
Clostridium tetanomorphum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.59 | - |
mesaconate | - |
Clostridium tetanomorphum | |
0.95 | - |
fumarate | - |
Clostridium tetanomorphum | |
2.4 | - |
L-citramalate | - |
Clostridium tetanomorphum | |
6.7 | - |
L-malate | - |
Clostridium tetanomorphum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | activates | Clostridium tetanomorphum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme consists of two components: component I has MW 45000 and component II has MW 310000, determined by gel filtration. A combination of both components gives the active enzyme. At pH 7.5 or below component II partially dissociates into species with molecular weights of about 165000, 82000, and 37000. At pH 9.0 these species reassociate to form active component II of about the original MW. The observed species may represent a monomer, dimer, tetramer and octamer of a single subunit | Clostridium tetanomorphum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium tetanomorphum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
component I and II | Clostridium tetanomorphum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(L)-citramalate | - |
Clostridium tetanomorphum | mesaconate + H2O | - |
? | |
(S)-malate | - |
Clostridium tetanomorphum | fumarate + H2O | - |
? | |
Citramalate + H2O | L-citramalate | Clostridium tetanomorphum | Mesaconate | - |
? | |
fumarate + H2O | - |
Clostridium tetanomorphum | L-malate | - |
? | |
mesaconate | - |
Clostridium tetanomorphum | citramalate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | the enzyme consists of two components: component I has MW 45000 and component II has MW 310000, determined by gel filtration. A combination of both components gives the active enzyme. At pH 7.5 or below component II partially dissociates into species with molecular weights of about 165000, 82000, and 37000. At pH 9.0 these species reassociate to form active component II of about the original MW. The observed species may represent a monomer, dimer, tetramer and octamer of a single subunit | Clostridium tetanomorphum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
at pH 7.5 or below component II partially dissociates into species with molecular weights of about 165000 Da, 82000 Da, and 37000 Da. At pH 9.0 these species reassociate to form active component II of about the original MW. The observed species may represent a monomer, dimer, tetramer and octamer of a single subunit | Clostridium tetanomorphum |