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Literature summary for 4.2.1.34 extracted from

  • Blair, A.H.; Barker, H.A.
    Assay and purification of (+)-citramalate hydro-lyase components from Clostridium tetanomorphum (1966), J. Biol. Chem., 241, 400-408.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
sulfhydryl compounds required Clostridium tetanomorphum

Inhibitors

Inhibitors Comment Organism Structure
diphosphate
-
 Clostridium tetanomorphum
phosphate
-
 Clostridium tetanomorphum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2 4 (+)-citramalate
-
 Clostridium tetanomorphum

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Clostridium tetanomorphum
Fe2+ Km for ferrous ammonium sulfate: 0.004 mM Clostridium tetanomorphum

Organism

Organism UniProt Comment Textmining
Clostridium tetanomorphum
-
-
-

Oxidation Stability

Oxidation Stability Organism
inactivation by exposure to oxygen, full activation by anaerobic incubation with a sulfhydryl compound for 1-2 hours at 37°C Clostridium tetanomorphum

Purification (Commentary)

Purification (Comment) Organism
component I and II Clostridium tetanomorphum
whole enzyme Clostridium tetanomorphum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24
-
-
 Clostridium tetanomorphum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(L)-citramalate
-
 Clostridium tetanomorphum mesaconate + H2O
-
 ?
Citramalate + H2O (+)-citramalate Clostridium tetanomorphum Mesaconate
-
 ?
mesaconate
-
 Clostridium tetanomorphum citramalate + H2O (+)-citramalate ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2 8.5
-
 Clostridium tetanomorphum