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show all sequences of 4.2.1.31

The substrate specificity of maleate hydratase from Arthrobacter sp. strain MCI2612

Ueda, M.; Yamada, H.; Asano, Y.; Appl. Microbiol. Biotechnol. 41, 215-218 (1994)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
use for synthesis of chiral synthons considered
Arthrobacter sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
(2R,3S)-tartaric acid
Ki: 3.15 mM
Arthrobacter sp.
acetylenedicarboxylate
Ki: 4.55 mM
Arthrobacter sp.
additional information
-
Arthrobacter sp.
oxaloacetic acid
Ki: 8.22 mM
Arthrobacter sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.74
-
2-chloromaleate
-
Arthrobacter sp.
3.27
-
2-bromomaleate
-
Arthrobacter sp.
3.85
-
Maleate
-
Arthrobacter sp.
4.55
-
citraconate
-
Arthrobacter sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
alpha1 beta1, 1 * 58000 + 1 * 28000, SDS-PAGE
Arthrobacter sp.
58000
-
alpha1 beta1, 1 * 58000 + 1 * 28000, SDS-PAGE
Arthrobacter sp.
90000
-
gel filtration
Arthrobacter sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arthrobacter sp.
-
-
-
Arthrobacter sp. MCI2612
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
bromomaleate
-
5648
Arthrobacter sp.
(-)-erythro((2S, 3S)-3-)-bromomalate
-
-
-
-
bromomaleate
-
5648
Arthrobacter sp. MCI2612
(-)-erythro((2S, 3S)-3-)-bromomalate
-
-
-
-
chloromaleate
-
5648
Arthrobacter sp.
(-)-erythro((2S, 3S)-3-)-chloromalate
-
-
-
-
chloromaleate
-
5648
Arthrobacter sp. MCI2612
(-)-erythro((2S, 3S)-3-)-chloromalate
-
-
-
-
citraconate
-
5648
Arthrobacter sp.
D-citramalate
-
5648
Arthrobacter sp.
-
citraconate
-
5648
Arthrobacter sp. MCI2612
D-citramalate
-
5648
Arthrobacter sp. MCI2612
-
Subunits
Subunits
Commentary
Organism
dimer
alpha1 beta1, 1 * 58000 + 1 * 28000, SDS-PAGE
Arthrobacter sp.
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
45
-
-
Arthrobacter sp.
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.15
-
(2R,3S)-tartaric acid
-
Arthrobacter sp.
4.55
-
acetylenedicarboxylate
-
Arthrobacter sp.
8.22
-
oxaloacetic acid
-
Arthrobacter sp.
Application (protein specific)
Application
Commentary
Organism
synthesis
use for synthesis of chiral synthons considered
Arthrobacter sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(2R,3S)-tartaric acid
Ki: 3.15 mM
Arthrobacter sp.
acetylenedicarboxylate
Ki: 4.55 mM
Arthrobacter sp.
additional information
-
Arthrobacter sp.
oxaloacetic acid
Ki: 8.22 mM
Arthrobacter sp.
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.15
-
(2R,3S)-tartaric acid
-
Arthrobacter sp.
4.55
-
acetylenedicarboxylate
-
Arthrobacter sp.
8.22
-
oxaloacetic acid
-
Arthrobacter sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.74
-
2-chloromaleate
-
Arthrobacter sp.
3.27
-
2-bromomaleate
-
Arthrobacter sp.
3.85
-
Maleate
-
Arthrobacter sp.
4.55
-
citraconate
-
Arthrobacter sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
alpha1 beta1, 1 * 58000 + 1 * 28000, SDS-PAGE
Arthrobacter sp.
58000
-
alpha1 beta1, 1 * 58000 + 1 * 28000, SDS-PAGE
Arthrobacter sp.
90000
-
gel filtration
Arthrobacter sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
bromomaleate
-
5648
Arthrobacter sp.
(-)-erythro((2S, 3S)-3-)-bromomalate
-
-
-
-
bromomaleate
-
5648
Arthrobacter sp. MCI2612
(-)-erythro((2S, 3S)-3-)-bromomalate
-
-
-
-
chloromaleate
-
5648
Arthrobacter sp.
(-)-erythro((2S, 3S)-3-)-chloromalate
-
-
-
-
chloromaleate
-
5648
Arthrobacter sp. MCI2612
(-)-erythro((2S, 3S)-3-)-chloromalate
-
-
-
-
citraconate
-
5648
Arthrobacter sp.
D-citramalate
-
5648
Arthrobacter sp.
-
citraconate
-
5648
Arthrobacter sp. MCI2612
D-citramalate
-
5648
Arthrobacter sp. MCI2612
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
alpha1 beta1, 1 * 58000 + 1 * 28000, SDS-PAGE
Arthrobacter sp.
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
45
-
-
Arthrobacter sp.
Other publictions for EC 4.2.1.31
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746830
Liu
Identification of a specific ...
Pseudomonas alcaligenes, Pseudomonas alcaligenes NCIMB 9867
Appl. Environ. Microbiol.
81
5753-5760
2015
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1
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2
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5
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1
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4
1
1
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1
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1
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2
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1
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4
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
5650
Van der Werf
-
Induction of maleate hydratase ...
Pseudomonas pseudoalcaligenes
World J. Microbiol. Biotechnol.
13
279-282
1997
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-
-
-
-
-
-
-
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1
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1
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1
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1
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1
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-
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-
-
5645
Van der Werf
-
Effect of maleate counterion o ...
Pseudomonas pseudoalcaligenes
Enzyme Microb. Technol.
17
430-436
1995
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1
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7
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1
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2
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1
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7
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-
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2
-
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-
-
-
-
-
-
-
-
-
5648
Ueda
-
The substrate specificity of m ...
Arthrobacter sp., Arthrobacter sp. MCI2612
Appl. Microbiol. Biotechnol.
41
215-218
1994
-
1
-
-
-
-
4
4
-
-
3
-
-
3
-
-
-
-
-
-
-
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6
1
1
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-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
-
4
3
4
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-
3
-
-
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-
-
-
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-
6
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
5647
Van der Werf
Purification and characterizat ...
Pseudomonas pseudoalcaligenes
Appl. Environ. Microbiol.
59
2823-2829
1993
1
-
-
-
-
1
10
2
-
-
3
-
-
2
-
-
1
-
-
-
1
-
3
1
2
-
-
-
2
-
-
-
2
-
-
1
-
-
-
-
-
1
-
10
2
2
-
-
3
-
-
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1
-
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1
-
3
1
2
-
-
-
2
-
-
-
-
-
-
-
-
-
5649
Ueda
-
Purification and characterizat ...
Arthrobacter sp., Arthrobacter sp. MCI2612
Biosci. Biotechnol. Biochem.
57
1545-1548
1993
1
-
-
-
-
-
8
1
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3
-
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3
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1
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1
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-
-
-
-
1
-
1
1
1
-
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1
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8
-
1
-
3
-
-
-
-
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1
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1
-
-
-
-
-
1
-
1
1
1
-
-
-
-
-
-
-
5646
Van der Werf
Screening for microorganisms p ...
Acinetobacter sp., Actinoplanes sp., Aspergillus sp., Brevibacterium sp., Candida sp. (in: Saccharomycetales), Corynebacterium sp., Exophiala sp., Hansenula sp., Klebsiella sp., Micrococcus sp., Mycobacterium sp., Nocardia sp., Penicillium sp., Phanerochaete sp., Pichia sp., Pleurotus sp., Proteus sp., Pseudomonas pseudoalcaligenes, Pseudomonas sp., Rhodotorula sp., Saccharomyces sp., Schizosaccharomyces sp., Sporobolomyces sp. (in: Microbotryomycetes), Streptomyces sp., Trichosporon sp., Xanthobacter sp., Yarrowia sp.
Appl. Environ. Microbiol.
58
2854-2860
1992
-
1
-
-
-
-
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-
-
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27
-
28
-
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26
-
-
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28
-
28
-
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27
-
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1
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27
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28
-
28
-
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27
-
-
-
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-
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5643
Dreyer
Isolation and biochemical char ...
Oryctolagus cuniculus
Eur. J. Biochem.
150
145-154
1985
1
-
-
-
-
1
12
-
1
4
2
1
-
3
-
-
1
1
-
1
1
-
2
2
1
-
-
-
-
1
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1
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1
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12
-
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1
4
2
1
-
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1
-
1
1
-
2
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
5641
Britten
Anion activation of maleate hy ...
Oryctolagus cuniculus
Biochim. Biophys. Acta
185
220-227
1969
1
1
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-
-
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1
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1
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1
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1
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1
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1
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1
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1
1
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1
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1
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1
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1
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1
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5644
Englard
Stereochemical curse of the ma ...
Oryctolagus cuniculus
J. Biol. Chem.
242
2255-2259
1967
-
-
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1
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1
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1
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1
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1
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1
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-
-
5642
Sacks
Malease, a hydrase from corn k ...
Zea mays
J. Biol. Chem.
192
231-236
1951
-
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1
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2
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2
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1
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1
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1
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