Literature summary for 4.2.1.31 extracted from

Ueda, M.; Yamada, H.; Asano, Y.
The substrate specificity of maleate hydratase from Arthrobacter sp. strain MCI2612 (1994), Appl. Microbiol. Biotechnol., 41, 215-218.

No PubMed abstract available

Search for more literature for 4.2.1.31

Application

Application	Comment	Organism
synthesis	use for synthesis of chiral synthons considered	Arthrobacter sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
(2R,3S)-tartaric acid	Ki: 3.15 mM	Arthrobacter sp.
acetylenedicarboxylate	Ki: 4.55 mM	Arthrobacter sp.
additional information	-	Arthrobacter sp.
oxaloacetic acid	Ki: 8.22 mM	Arthrobacter sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.74	-	2-chloromaleate	-	Arthrobacter sp.
3.27	-	2-bromomaleate	-	Arthrobacter sp.
3.85	-	Maleate	-	Arthrobacter sp.
4.55	-	citraconate	-	Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	-	alpha1 beta1, 1 * 58000 + 1 * 28000, SDS-PAGE	Arthrobacter sp.
58000	-	alpha1 beta1, 1 * 58000 + 1 * 28000, SDS-PAGE	Arthrobacter sp.
90000	-	gel filtration	Arthrobacter sp.

Organism

Organism	UniProt	Comment	Textmining
Arthrobacter sp.	-	-	-
Arthrobacter sp. MCI2612	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
bromomaleate	-	Arthrobacter sp.	(-)-erythro((2S, 3S)-3-)-bromomalate	-	?
bromomaleate	-	Arthrobacter sp. MCI2612	(-)-erythro((2S, 3S)-3-)-bromomalate	-	?
chloromaleate	-	Arthrobacter sp.	(-)-erythro((2S, 3S)-3-)-chloromalate	-	?
chloromaleate	-	Arthrobacter sp. MCI2612	(-)-erythro((2S, 3S)-3-)-chloromalate	-	?
citraconate	-	Arthrobacter sp.	D-citramalate	-	?
citraconate	-	Arthrobacter sp. MCI2612	D-citramalate	-	?
maleate + H2O	-	Arthrobacter sp.	(R)-malate	-	r
maleate + H2O	-	Arthrobacter sp. MCI2612	(R)-malate	-	r

Subunits

Subunits	Comment	Organism
dimer	alpha1 beta1, 1 * 58000 + 1 * 28000, SDS-PAGE	Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	-	-	Arthrobacter sp.

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
3.15	-	(2R,3S)-tartaric acid	-	Arthrobacter sp.
4.55	-	acetylenedicarboxylate	-	Arthrobacter sp.
8.22	-	oxaloacetic acid	-	Arthrobacter sp.

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Release 2023.1 (January 2023)