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Literature summary for 4.2.1.30 extracted from
- Spectrophotometric assay for sensitive detection of glycerol dehydratase activity using aldehyde dehydrogenase (2017), J. Biosci. Bioeng., 123, 528-533 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the dhaB gene in Escherichia coli BL21(DE3) | Klebsiella pneumoniae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.73 | - |
glycerol | pH 7.0, 22°C | Klebsiella pneumoniae |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella pneumoniae | Q59476 AND O08505 AND Q59475 | Q59476: alpha-subunit, O08505: beta-subunit, Q59475: gamma-subunit | - |
| Klebsiella pneumoniae DSM 2026 | Q59476 AND O08505 AND Q59475 | Q59476: alpha-subunit, O08505: beta-subunit, Q59475: gamma-subunit | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
spectrophotometric assay for the sensitive measurement of the glycerol dehydratase activity with a sub-nanomolar limit of detection. The assay method employs aldehyde dehydrogenase as a reporter enzyme, so the readout of the glycerol dehydratase activity is recorded at 340 nm as an increase in UV absorbance which results from NADH generation accompanied by oxidation of 3-hydroxypropionaldehyde to 3-hydroxypropionic acid. The glycerol dehydratase assay is performed under the reaction conditions where the aldehyde dehydrogenase activity overwhelms the GDHt activity (i.e., 50fold higher activity of aldehyde dehydrogenase relative to glycerol dehydratase activity), affording sensitive detection of glycerol dehydratase with 360 pM limit of detection | Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerol | - |
Klebsiella pneumoniae | 3-hydroxypropanal + H2O | - |
? | |
| glycerol | - |
Klebsiella pneumoniae DSM 2026 | 3-hydroxypropanal + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDHt | - |
Klebsiella pneumoniae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 400 | - |
glycerol | pH 7.0, 22°C | Klebsiella pneumoniae | |
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