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Literature summary for 4.2.1.30 extracted from
- In situ reactivation of glycerol-inactivated coenzyme B12-dependent enzymes, glycerol dehydratase and diol dehydratase (1980), J. Bacteriol., 143, 1458-1465.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glycerol | inactivation of the enzyme in situ and in vitro during catalysis, glycerol inactivated enzyme in situ is reactivated by ATP and Mn2+ in the presence of free adenosylcobalamin. CTP and GTP are able to replace ATP partially. Mn2+ can be fully replaced by Mg2+ | Klebsiella pneumoniae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
1,2-propanediol | enzyme in situ, toluene-treated cells | Klebsiella pneumoniae | |
| 0.36 | - |
1,2-propanediol | enzyme in vitro | Klebsiella pneumoniae | |
| 0.53 | - |
glycerol | enzyme in situ, toluene-treated cells | Klebsiella pneumoniae | |
| 0.54 | - |
glycerol | enzyme in vitro | Klebsiella pneumoniae | |
| 1.08 | - |
ethanediol | enzyme in situ, toluene-treated cells | Klebsiella pneumoniae | |
| 1.56 | - |
ethanediol | enzyme in vitro | Klebsiella pneumoniae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerol | Klebsiella pneumoniae | - |
? | - |
? | |
| glycerol | Klebsiella pneumoniae PZH 572 Warsaw | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella pneumoniae | - |
available as Enterobacter aerogenes ATCC 25955 | - |
| Klebsiella pneumoniae | - |
ATCC 25955 | - |
| Klebsiella pneumoniae PZH 572 Warsaw | - |
available as Enterobacter aerogenes ATCC 25955 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-ethanediol | - |
Klebsiella pneumoniae | acetaldehyde + H2O | - |
? | |
| 1,2-ethanediol | - |
Klebsiella pneumoniae PZH 572 Warsaw | acetaldehyde + H2O | - |
? | |
| 1,2-propanediol | - |
Klebsiella pneumoniae | propionaldehyde + H2O | - |
? | |
| 1,2-propanediol | - |
Klebsiella pneumoniae PZH 572 Warsaw | propionaldehyde + H2O | - |
? | |
| glycerol | - |
Klebsiella pneumoniae | 3-hydroxypropanal + H2O | - |
? | |
| glycerol | - |
Klebsiella pneumoniae PZH 572 Warsaw | 3-hydroxypropanal + H2O | - |
? | |
| glycerol | - |
Klebsiella pneumoniae | ? | - |
? | |
| glycerol | - |
Klebsiella pneumoniae PZH 572 Warsaw | ? | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 5,6-Dimethylbenzimidazolylcobamide | coenzyme required | Klebsiella pneumoniae | |
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