Literature summary for 4.2.1.3 extracted from

Pitula, J.S.; Deck, K.M.; Clarke, S.L.; Anderson, S.A.; Vasanthakumar, A.; Eisenstein, R.S.
Selective inhibition of the citrate-to-isocitrate reaction of cytosolic aconitase by phosphomimetic mutation of serine-711 (2004), Proc. Natl. Acad. Sci. USA, 101, 10907-10912.

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Protein Variants

Protein Variants	Comment	Organism
S711A	citrate-to-isocitrate aconitase activity is about 70% of the wild-type activity, isocitrate-to-cis-aconitate activity is about 90% of wild-type activity	Homo sapiens
S711D	no citrate-to-isocitrate aconitase activity, isocitrate-to-cis-aconitate activity is identical to wild-type activity	Homo sapiens
S711E	citrate-to-isocitrate aconitase activity is about 10% of the wild-type activity, isocitrate-to-cis-aconitate activity is about 20% of wild-type activity	Homo sapiens
S711T	citrate-to-isocitrate aconitase activity is about 60% of the wild-type activity, isocitrate-to-cis-aconitate activity is about 60% of wild-type activity	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Homo sapiens	5829	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	S711 of IRP1 is phosphorylated by proteinkinase C in vitro and in vivo in human HEK cells. Aconitase activity of the S711 phosphomimetic mutants is preferentially inhibited in the citrate mode of aconitase function. The substitution of phosphomimetic amino acids for S711 fails to affect the RNA-binding affinity of IRP1	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
HEK-293 cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
citrate	-	Homo sapiens	isocitrate	-	r
isocitrate	-	Homo sapiens	cis-aconitate + H2O	-	r

Synonyms

Synonyms	Comment	Organism
c-acon	-	Homo sapiens
iron-regulatory protein 1	-	Homo sapiens
IRP1	-	Homo sapiens

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Release 2023.1 (January 2023)