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Literature summary for 4.2.1.28 extracted from

  • Wei, X.; Meng, X.; Chen, Y.; Wei, Y.; Du, L.; Huang, R.
    Cloning, expression, and characterization of coenzyme-B12-dependent diol dehydratase from Lactobacillus diolivorans (2014), Biotechnol. Lett., 36, 159-165.
    View publication on PubMed
Search for more literature for 4.2.1.28

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Lentilactobacillus diolivorans

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Lentilactobacillus diolivorans
glycerol suicide inactivation, Km value of 5.8 mM Lentilactobacillus diolivorans
Mg2+ slight inhibition Lentilactobacillus diolivorans
Mn2+
-
 Lentilactobacillus diolivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 Propane-1,2-diol at pH 8.6 and 37°C Lentilactobacillus diolivorans
10.5
-
 ethane-1,2-diol at pH 8.6 and 37°C Lentilactobacillus diolivorans

Metals/Ions

Metals/Ions Comment Organism Structure
K+ enzyme activity increases by 1 mM K+ Lentilactobacillus diolivorans
additional information not affected by Na+ and Li+ Lentilactobacillus diolivorans
NH4+ enzyme activity increases by 1 mM NH4+ Lentilactobacillus diolivorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19800
-
 2 * 61100 + 2 * 35000 + 2 * 19800, SDS-PAGE Lentilactobacillus diolivorans
35000
-
 2 * 61100 + 2 * 35000 + 2 * 19800, SDS-PAGE Lentilactobacillus diolivorans
61100
-
 2 * 61100 + 2 * 35000 + 2 * 19800, SDS-PAGE Lentilactobacillus diolivorans
210000
-
 gel filtration Lentilactobacillus diolivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
propane-1,2-diol Lentilactobacillus diolivorans
-
 propanal + H2O
-
 ?
propane-1,2-diol Lentilactobacillus diolivorans DSM 14421
-
 propanal + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Lentilactobacillus diolivorans
-
-
-
Lentilactobacillus diolivorans DSM 14421
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethane-1,2-diol
-
 Lentilactobacillus diolivorans ethanal + H2O
-
 ?
ethane-1,2-diol
-
 Lentilactobacillus diolivorans DSM 14421 ethanal + H2O
-
 ?
propane-1,2-diol
-
 Lentilactobacillus diolivorans propanal + H2O
-
 ?
propane-1,2-diol
-
 Lentilactobacillus diolivorans DSM 14421 propanal + H2O
-
 ?

Subunits

Subunits Comment Organism
heterohexamer 2 * 61100 + 2 * 35000 + 2 * 19800, SDS-PAGE Lentilactobacillus diolivorans

Synonyms

Synonyms Comment Organism
coenzyme-B12-dependent diol dehydratase
-
 Lentilactobacillus diolivorans
DDH
-
 Lentilactobacillus diolivorans
GldCDE
-
 Lentilactobacillus diolivorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Lentilactobacillus diolivorans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
16 50 the enzyme retains more than 70% of its activity between 30 and 40°C but less than 20% of its activity at 16 and 50°C Lentilactobacillus diolivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
-
 Lentilactobacillus diolivorans

pH Range

pH Minimum pH Maximum Comment Organism
4.5 10.5 less than 10% activity is observed at pH 4.5 and pH 10.5 Lentilactobacillus diolivorans

Cofactor

Cofactor Comment Organism Structure
coenzyme B12 dependent on, Km value of 0.0015 mM Lentilactobacillus diolivorans