Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Klebsiella oxytoca |
Crystallization (Comment) | Organism |
---|---|
sandwich-drop vapor diffusion method, 4ΒΊC | Klebsiella oxytoca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella oxytoca | - |
- |
- |
Purification (Comment) | Organism |
---|---|
chromatography on DEAE-cellulose | Klebsiella oxytoca |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
propane-1,2-diol = propanal + H2O | the enzyme-bound adenosylcobalamin serves as an intermediate hydrogen carrier, accepting a hydrogen atom from C1 of the substrate to C5' of the coenzyme and giving a hydrogen back to C2 of the product. R and S enantiomers are bound to the active site of the enzyme in a symmetrical mode with respect to the plane | Klebsiella oxytoca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-propanediol | (S) and (R) stereoisomers | Klebsiella oxytoca | propanal | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme B12 | adenosylcobalamin | Klebsiella oxytoca |