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Literature summary for 4.2.1.28 extracted from

  • Hartmanis, M.G.N.; Stadtman, T.C.
    Diol metabolism and diol dehydratase in Clostridium glycoliticum (1986), Arch. Biochem. Biophys., 245, 144-152.
    View publication on PubMed
Search for more literature for 4.2.1.28

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activates, 900% stimulation at 1 mM Terrisporobacter glycolicus

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Terrisporobacter glycolicus
EGTA 62% inhibition at 0.05 mM Terrisporobacter glycolicus
ethylene glycol bis -N,N,N,N-tetraacetic acid beta-aminoethyl ether Terrisporobacter glycolicus
hydroxylamine
-
 Terrisporobacter glycolicus
Hydroxyurea
-
 Terrisporobacter glycolicus
iodoacetamide complete inhibition at 5 mM Terrisporobacter glycolicus
o-phenanthroline
-
 Terrisporobacter glycolicus
p-chloromercuriphenylsulfonic acid complete inhibition at 0.1 mM Terrisporobacter glycolicus
sulfhydryl compounds
-
 Terrisporobacter glycolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3
-
 1,2-ethanediol
-
 Terrisporobacter glycolicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cell membrane associated with Terrisporobacter glycolicus
-
-
cell membrane intergral membrane protein Terrisporobacter glycolicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
divalent cations
-
 Terrisporobacter glycolicus
K+ no requirement Terrisporobacter glycolicus

Organism

Organism UniProt Comment Textmining
Terrisporobacter glycolicus
-
-
-

Oxidation Stability

Oxidation Stability Organism
extremely O2 sensitive Terrisporobacter glycolicus

Reaction

Reaction Comment Organism Reaction ID
propane-1,2-diol = propanal + H2O radical mechanism involved Terrisporobacter glycolicus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Terrisporobacter glycolicus
1.5
-
 under strictly anaerobic conditions Terrisporobacter glycolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-ethanediol
-
 Terrisporobacter glycolicus acetaldehyde
-
 ?
1,2-propanediol
-
 Terrisporobacter glycolicus propanal
-
 ?
additional information specific for 1,2-ethanediol and 1,2-propanediol Terrisporobacter glycolicus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 enzyme assay at Terrisporobacter glycolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Terrisporobacter glycolicus

Cofactor

Cofactor Comment Organism Structure
cobamide not involved Terrisporobacter glycolicus