BRENDA - Enzyme Database
show all sequences of 4.2.1.28

Diol metabolism and diol dehydratase in Clostridium glycoliticum

Hartmanis, M.G.N.; Stadtman, T.C.; Arch. Biochem. Biophys. 245, 144-152 (1986)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
dithiothreitol
activates, 900% stimulation at 1 mM
Terrisporobacter glycolicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
-
Terrisporobacter glycolicus
EGTA
62% inhibition at 0.05 mM
Terrisporobacter glycolicus
ethylene glycol bis -N,N,N,N-tetraacetic acid
beta-aminoethyl ether
Terrisporobacter glycolicus
hydroxylamine
-
Terrisporobacter glycolicus
Hydroxyurea
-
Terrisporobacter glycolicus
iodoacetamide
complete inhibition at 5 mM
Terrisporobacter glycolicus
o-phenanthroline
-
Terrisporobacter glycolicus
p-chloromercuriphenylsulfonic acid
complete inhibition at 0.1 mM
Terrisporobacter glycolicus
sulfhydryl compounds
-
Terrisporobacter glycolicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3
-
1,2-ethanediol
-
Terrisporobacter glycolicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cell membrane
associated with; intergral membrane protein
Terrisporobacter glycolicus
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
divalent cations
-
Terrisporobacter glycolicus
K+
no requirement
Terrisporobacter glycolicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Terrisporobacter glycolicus
-
-
-
Oxidation Stability
Oxidation Stability
Organism
extremely O2 sensitive
Terrisporobacter glycolicus
Reaction
Reaction
Commentary
Organism
propane-1,2-diol = propanal + H2O
radical mechanism involved
Terrisporobacter glycolicus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Terrisporobacter glycolicus
1.5
-
under strictly anaerobic conditions
Terrisporobacter glycolicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-ethanediol
-
5628
Terrisporobacter glycolicus
acetaldehyde
-
-
-
-
1,2-propanediol
-
5628
Terrisporobacter glycolicus
propanal
-
5628
Terrisporobacter glycolicus
-
additional information
specific for 1,2-ethanediol and 1,2-propanediol
5628
Terrisporobacter glycolicus
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
enzyme assay at
Terrisporobacter glycolicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Terrisporobacter glycolicus
Cofactor
Cofactor
Commentary
Organism
Structure
cobamide
not involved
Terrisporobacter glycolicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
dithiothreitol
activates, 900% stimulation at 1 mM
Terrisporobacter glycolicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cobamide
not involved
Terrisporobacter glycolicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
-
Terrisporobacter glycolicus
EGTA
62% inhibition at 0.05 mM
Terrisporobacter glycolicus
ethylene glycol bis -N,N,N,N-tetraacetic acid
beta-aminoethyl ether
Terrisporobacter glycolicus
hydroxylamine
-
Terrisporobacter glycolicus
Hydroxyurea
-
Terrisporobacter glycolicus
iodoacetamide
complete inhibition at 5 mM
Terrisporobacter glycolicus
o-phenanthroline
-
Terrisporobacter glycolicus
p-chloromercuriphenylsulfonic acid
complete inhibition at 0.1 mM
Terrisporobacter glycolicus
sulfhydryl compounds
-
Terrisporobacter glycolicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3
-
1,2-ethanediol
-
Terrisporobacter glycolicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cell membrane
associated with; intergral membrane protein
Terrisporobacter glycolicus
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
divalent cations
-
Terrisporobacter glycolicus
K+
no requirement
Terrisporobacter glycolicus
Oxidation Stability (protein specific)
Oxidation Stability
Organism
extremely O2 sensitive
Terrisporobacter glycolicus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Terrisporobacter glycolicus
1.5
-
under strictly anaerobic conditions
Terrisporobacter glycolicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-ethanediol
-
5628
Terrisporobacter glycolicus
acetaldehyde
-
-
-
-
1,2-propanediol
-
5628
Terrisporobacter glycolicus
propanal
-
5628
Terrisporobacter glycolicus
-
additional information
specific for 1,2-ethanediol and 1,2-propanediol
5628
Terrisporobacter glycolicus
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
enzyme assay at
Terrisporobacter glycolicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Terrisporobacter glycolicus
Other publictions for EC 4.2.1.28
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746754
Shibata
Direct participation of a per ...
Klebsiella oxytoca
Angew. Chem. Int. Ed. Engl.
57
7830-7835
2018
-
-
-
1
5
-
-
5
-
-
-
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1
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1
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6
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1
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1
1
5
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5
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-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
747141
Levin
Characterization of 1,2-propa ...
Klebsiella oxytoca, Klebsiella oxytoca ATCC 8724, Roseburia inulinivorans, Roseburia inulinivorans A2-194
Biochemistry
57
3222-3226
2018
-
-
2
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
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2
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2
2
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-
-
-
-
-
-
-
-
-
2
2
-
-
-
747115
Toraya
Diol dehydratase-reactivase i ...
Klebsiella oxytoca, Klebsiella oxytoca ATCC 8724
Biochemistry
55
69-78
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
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-
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-
-
-
-
-
-
1
1
-
-
-
747443
Doitomi
-
Computational mutation study ...
Klebsiella oxytoca
Bull. Chem. Soc. JPN
89
955-964
2016
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
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4
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-
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-
-
-
-
-
729472
Wei
Cloning, expression, and chara ...
Lactobacillus diolivorans, Lactobacillus diolivorans DSM 14421
Biotechnol. Lett.
36
159-165
2014
-
-
1
-
-
-
4
2
-
3
4
2
-
4
-
-
-
-
-
-
-
-
4
1
1
1
-
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
4
-
2
-
3
4
2
-
-
-
-
-
-
-
-
4
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
749131
Ghiaci
2-Butanol and butanone produc ...
Saccharomyces cerevisiae
PLoS ONE
9
e102774
2014
-
1
-
-
-
-
-
-
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-
1
-
-
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-
-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
729706
Yamanishi
Redesign of coenzyme B12 depen ...
Klebsiella oxytoca, Klebsiella oxytoca ATCC 8724
FEBS J.
279
793-804
2012
-
-
1
1
3
-
1
8
-
-
-
4
-
2
-
-
1
-
-
-
-
-
8
-
-
-
-
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1
-
-
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1
1
1
3
-
-
1
-
8
-
-
-
4
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715171
Kamachi
Catalytic roles of the metal i ...
Klebsiella oxytoca
Inorg. Chem.
50
2944-2952
2011
-
-
-
-
-
-
1
-
-
3
-
1
-
1
-
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1
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3
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1
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3
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1
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-
3
-
-
-
-
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-
-
-
-
-
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-
-
-
-
715388
Fan
The N-terminal region of the m ...
Salmonella enterica
J. Bacteriol.
193
5623-5628
2011
-
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1
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1
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-
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1
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2
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1
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1
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1
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1
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1
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1
1
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1
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1
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1
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-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
714211
Toraya
Coenzyme B12-dependent diol de ...
Klebsiella oxytoca, Klebsiella oxytoca ATCC 8724
Biochemistry
49
7210-7217
2010
-
-
1
-
-
1
3
-
-
10
1
2
-
3
-
-
-
-
1
1
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
1
-
3
-
-
-
10
1
2
-
-
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-
1
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
714968
Mori
Diol dehydratase-reactivating ...
Klebsiella oxytoca
FEBS J.
277
4931-4943
2010
1
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2
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3
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3
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6
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695923
Tobimatsu
Low-solubility glycerol dehydr ...
Klebsiella oxytoca, Klebsiella pneumoniae, Salmonella enterica
Arch. Microbiol.
191
199-206
2008
-
-
3
-
3
-
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3
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3
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6
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3
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3
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3
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3
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3
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6
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3
-
-
-
3
-
-
-
-
-
-
-
-
-
696252
Kinoshita
Histidine-alpha143 assists 1,2 ...
Klebsiella oxytoca
Biochemistry
47
3162-3173
2008
-
-
1
-
5
-
2
5
-
1
-
1
-
3
1
-
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1
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-
-
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2
-
1
-
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4
1
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1
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1
1
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5
-
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2
-
5
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1
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1
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1
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-
-
-
-
2
-
1
-
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4
1
-
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-
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-
-
697886
Ogura
Roles of adenine anchoring and ...
Klebsiella oxytoca
FEBS J.
275
6204-6216
2008
-
-
-
-
6
-
2
5
-
1
-
1
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
7
1
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-
1
7
-
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1
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6
-
-
2
7
5
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1
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1
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2
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1
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7
1
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698588
Sriramulu
Lactobacillus reuteri DSM 2001 ...
Lactobacillus reuteri
J. Bacteriol.
190
4559-4567
2008
-
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1
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1
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8
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1
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2
1
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1
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1
1
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1
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1
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2
1
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698651
Toraya
Mechanism-based inactivation o ...
Klebsiella oxytoca
J. Biochem.
144
437-446
2008
-
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1
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5
3
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1
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1
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1
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1
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5
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1
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2
1
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1
5
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1
1
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5
5
3
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1
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1
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1
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5
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1
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2
1
-
-
-
-
-
-
-
-
-
698752
Parsons
Biochemical and structural ins ...
Citrobacter freundii
J. Biol. Chem.
283
14366-14375
2008
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-
1
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1
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3
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3
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3
1
1
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1
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3
3
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3
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3
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3
3
3
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-
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672269
Schwartz
Dioldehydrase: an essential ro ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biochemistry
46
7293-7301
2007
1
-
1
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-
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-
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1
1
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1
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-
-
-
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1
1
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1
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1
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1
1
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1
1
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-
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-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673603
Kajiura
Molecular basis for specificit ...
Klebsiella oxytoca, Klebsiella pneumoniae
FEBS J.
274
5556-5566
2007
4
-
2
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-
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2
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2
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2
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6
-
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2
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4
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2
2
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2
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2
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6
-
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675575
Sakai
Construction and characterizat ...
Klebsiella oxytoca, Klebsiella pneumoniae
J. Nutr. Sci. Vitaminol.
53
102-108
2007
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2
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2
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4
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6
-
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2
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2
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2
2
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2
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4
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Biochemistry
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Radical catalysis of B12 enzym ...
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How a protein generates a cata ...
Klebsiella oxytoca
Structure Fold. Des.
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5630
Masuda
Crystallization and preliminar ...
Klebsiella pneumoniae
Acta Crystallogr. Sect. D
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A reactivating factor for coen ...
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Mechanism of reactivation of c ...
Klebsiella oxytoca
Biochemistry
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EPR spectroscopic evidence for ...
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5620
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Kinetic investigations with in ...
Klebsiella pneumoniae
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5635
Tobimatsu
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5614
Toraya
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5636
Hartmanis
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Diol dehydrase from Clostridiu ...
Terrisporobacter glycolicus
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5634
Ishida
Adenosylcobinamide methyl phos ...
Klebsiella pneumoniae
Biochemistry
32
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5633
Schramm
Ether-cleaving enzyme and diol ...
Acetobacterium sp.
Biodegradation
2
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1991
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5628
Hartmanis
Diol metabolism and diol dehyd ...
Terrisporobacter glycolicus
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5629
Tanizawa
Re-investigation of the protei ...
Klebsiella pneumoniae
Z. Naturforsch. C
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1986
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5627
Ichikawa
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Purification and properties of ...
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5626
Schuetz
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5623
Toraya
-
Diol dehydrase ...
Citrobacter freundii, Klebsiella pneumoniae, Propionibacterium freudenreichii
B12 (Dolphin, D. ed. ) Wiley, New York
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233-262
1982
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5624
Forage
Glycerol fermentation in Klebs ...
Klebsiella pneumoniae
J. Bacteriol.
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1982
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5625
McGee
Diol dehydratase: N-terminal a ...
Klebsiella pneumoniae
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108
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1982
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5622
McGee
Purification and subunit chara ...
Klebsiella pneumoniae
Biochemistry
20
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1981
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5
-
-
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-
1
-
-
1
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5621
Toraya
Inactivation of dioldehydrase ...
Klebsiella pneumoniae
Arch. Biochem. Biophys.
203
174-180
1980
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-
-
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1
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-
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1
-
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1
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1
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1
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1
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-
1
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-
-
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-
-
-
-
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-
-
5618
Poznanskaja
Coenzyme B12-dependent diol de ...
Klebsiella pneumoniae
Arch. Biochem. Biophys.
194
379-386
1979
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-
-
-
-
-
-
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-
-
3
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1
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1
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1
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1
1
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1
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1
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3
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1
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-
1
-
1
1
-
-
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-
5619
Moore
Stereospecificity and mechanis ...
Klebsiella pneumoniae
Biochem. Biophys. Res. Commun.
87
1052-1057
1979
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-
-
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1
1
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1
1
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2
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-
-
1
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1
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1
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1
-
1
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1
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-
-
2
-
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-
-
1
-
-
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-
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-
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-
5617
Bachovchin
Mechanism of action of Adenosy ...
Klebsiella pneumoniae
Biochemistry
16
1082-1092
1977
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-
-
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-
-
2
5
-
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-
1
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-
-
-
-
2
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-
-
2
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1
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1
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2
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5
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-
-
2
-
-
-
-
2
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-
5616
Toraya
Substrate specificity of coenz ...
Klebsiella pneumoniae
Biochem. Biophys. Res. Commun.
69
475-480
1976
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-
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1
4
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1
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-
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5
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5
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1
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1
-
-
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-
1
-
4
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-
-
-
-
-
-
5
-
-
-
-
5
-
-
-
-
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-
-
5615
Jensen
Relative enantiomer binding an ...
Klebsiella pneumoniae
Biochem. Biophys. Res. Commun.
62
816-821
1975
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-
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1
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1
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1
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1
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-
1
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-
-
-
-
5613
Toraya
Dissociation of diol dehydrase ...
Klebsiella pneumoniae
Biochem. Biophys. Res. Commun.
52
350-355
1973
-
-
-
-
-
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1
-
-
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1
1
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1
1
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1
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1
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1
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1
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-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5612
Toraya
Coenzyme B12-dependent propane ...
Klebsiella pneumoniae
Biochim. Biophys. Acta
284
536-548
1972
-
-
-
-
-
1
6
1
-
1
-
-
-
1
1
-
-
1
-
-
-
-
4
-
-
-
2
-
-
-
-
1
1
-
-
-
-
-
1
-
-
1
-
6
1
1
-
1
-
-
-
1
-
-
-
-
-
-
4
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
5611
Abeles
-
Dehydrations requiring vitamin ...
Klebsiella pneumoniae
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
5
481-497
1971
-
-
-
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1
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1
1
-
1
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1
1
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2
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1
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1
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1
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1
1
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1
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2
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