Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.28 extracted from

  • Ichikawa, Y.; Horike, Y.; Mori, N.; Hosoi, N.; Kitamoto, Y.
    Purification and properties of propanediol dehydratase from Propionibacterium freudenreichii (1985), J. Ferment. Technol., 63, 135-141.
No PubMed abstract available
Search for more literature for 4.2.1.28

Inhibitors

Inhibitors Comment Organism Structure
1,2-Butanediol
-
 Propionibacterium freudenreichii
1,2-cyclohexanediol
-
 Propionibacterium freudenreichii
1,2-ethanediol weak Propionibacterium freudenreichii
1,3-butanediol
-
 Propionibacterium freudenreichii
2-fluoroethanol weak Propionibacterium freudenreichii
coenzyme B12 inactivation in absence of substrate Propionibacterium freudenreichii
isopropanolamine weak Propionibacterium freudenreichii
additional information various chemicals and substrate analogues Propionibacterium freudenreichii
o-iodobenzoic acid
-
 Propionibacterium freudenreichii
p-chloromercuribenzoate 92% inhibition by 1 mM Propionibacterium freudenreichii
phenylhydrazine
-
 Propionibacterium freudenreichii
Sodium azide
-
 Propionibacterium freudenreichii
sulfhydryl compounds
-
 Propionibacterium freudenreichii
Thiourea
-
 Propionibacterium freudenreichii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Propionibacterium freudenreichii

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+ slight activation Propionibacterium freudenreichii
K+ requirement for monovalent cation Propionibacterium freudenreichii
Li+ slight activation Propionibacterium freudenreichii
Na+ slight activation Propionibacterium freudenreichii
NH4+
-
 Propionibacterium freudenreichii
Rb+
-
 Propionibacterium freudenreichii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
230000
-
 gel filtration Propionibacterium freudenreichii

Organism

Organism UniProt Comment Textmining
Propionibacterium freudenreichii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Propionibacterium freudenreichii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
197
-
-
 Propionibacterium freudenreichii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-butanediol poor substrate Propionibacterium freudenreichii butyraldehyde
-
 ?
1,2-ethanediol
-
 Propionibacterium freudenreichii acetaldehyde ? ?
1,2-propanediol
-
 Propionibacterium freudenreichii propanal
-
 ?
1,3-butanediol low turnover Propionibacterium freudenreichii ?
-
 ?
2,3-butanediol poor substrate Propionibacterium freudenreichii methyl ethyl ketone
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Propionibacterium freudenreichii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 90% loss of activity after 3 min Propionibacterium freudenreichii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.2
-
-
 Propionibacterium freudenreichii

pH Range

pH Minimum pH Maximum Comment Organism
7.2 11.2
-
 Propionibacterium freudenreichii

Cofactor

Cofactor Comment Organism Structure
cobamide
-
 Propionibacterium freudenreichii