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Literature summary for 4.2.1.28 extracted from

  • Toraya, T.
    Diol dehydrase and glycerol dehydrase, coenzyme B12-dependent isozymes (1994), Met. Ions Biol. Syst., 30, 217-254.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
glycerol suicide inactivation in vitro, reactivation possible in presence of coenzyme, Mg2+, ATP in vivo Klebsiella pneumoniae
additional information
-
Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
K+ requirement for monovalent cation Klebsiella pneumoniae
K+ crystal ionic radius determines effectiveness as activator Klebsiella pneumoniae
K+ stabilizes apoenzyme-coenzyme association Klebsiella pneumoniae
NH4+ very effective Klebsiella pneumoniae
Rb+
-
Klebsiella pneumoniae
Tl+
-
Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
F consisits of one polypeptide, S of at least four subunits, x * 60000, x * 23000, x * 15500, x * 14000, Cys, Lys, His, andArg residues essential for catalysis Klebsiella pneumoniae
15500
-
F consisits of one polypeptide, S of at least four subunits, x * 60000, x * 23000, x * 15500, x * 14000, Cys, Lys, His, andArg residues essential for catalysis Klebsiella pneumoniae
19173
-
x * 28071, x * 60348, x * 24113, x * 19173, estimated from nucleotide sequences of cloned genes Klebsiella pneumoniae
23000
-
F consisits of one polypeptide, S of at least four subunits, x * 60000, x * 23000, x * 15500, x * 14000, Cys, Lys, His, andArg residues essential for catalysis Klebsiella pneumoniae
24113
-
x * 28071, x * 60348, x * 24113, x * 19173, estimated from nucleotide sequences of cloned genes Klebsiella pneumoniae
26000
-
1 * 26000, 1 * 200000, components F, S Klebsiella pneumoniae
28071
-
x * 28071, x * 60348, x * 24113, x * 19173, estimated from nucleotide sequences of cloned genes Klebsiella pneumoniae
60000
-
F consisits of one polypeptide, S of at least four subunits, x * 60000, x * 23000, x * 15500, x * 14000, Cys, Lys, His, andArg residues essential for catalysis Klebsiella pneumoniae
60348
-
x * 28071, x * 60348, x * 24113, x * 19173, estimated from nucleotide sequences of cloned genes Klebsiella pneumoniae
200000
-
1 * 26000, 1 * 200000, components F, S Klebsiella pneumoniae
220000 240000 gel filtration, sedimentation equilibrium of cell-free extracts Klebsiella pneumoniae
250000
-
gel filtration of membrane extracts Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Klebsiella pneumoniae production of both electron acceptor and intermediate for fermentative oxidation of 1,2-diols ?
-
?

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
-
-
Klebsiella pneumoniae
-
also Klebsiella oxytoca
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Klebsiella pneumoniae

Reaction

Reaction Comment Organism Reaction ID
propane-1,2-diol = propanal + H2O general mechanism and stereochemistry, role of coenzyme in OH-group transfer, mechanism of overall reaction, role of free radicals during reaction, studies with coenzyme analogues Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-propanediol
-
Salmonella enterica subsp. enterica serovar Typhimurium propanal
-
?
1,2-propanediol
-
Klebsiella pneumoniae propanal
-
?
1,2-propanediol
-
Flavobacterium sp. propanal
-
?
additional information production of both electron acceptor and intermediate for fermentative oxidation of 1,2-diols Klebsiella pneumoniae ?
-
?

Subunits

Subunits Comment Organism
More 1 * 26000, 1 * 200000, components F, S Klebsiella pneumoniae
More F consisits of one polypeptide, S of at least four subunits, x * 60000, x * 23000, x * 15500, x * 14000, Cys, Lys, His, andArg residues essential for catalysis Klebsiella pneumoniae
oligomer x * 28071, x * 60348, x * 24113, x * 19173, estimated from nucleotide sequences of cloned genes Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
cobamide
-
Flavobacterium sp.
cobamide slow inactivation by analogues Klebsiella pneumoniae