Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glycerol | suicide inactivation in vitro, reactivation possible in presence of coenzyme, Mg2+, ATP in vivo | Klebsiella pneumoniae | |
additional information | - |
Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Klebsiella pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | requirement for monovalent cation | Klebsiella pneumoniae | |
K+ | crystal ionic radius determines effectiveness as activator | Klebsiella pneumoniae | |
K+ | stabilizes apoenzyme-coenzyme association | Klebsiella pneumoniae | |
NH4+ | very effective | Klebsiella pneumoniae | |
Rb+ | - |
Klebsiella pneumoniae | |
Tl+ | - |
Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
F consisits of one polypeptide, S of at least four subunits, x * 60000, x * 23000, x * 15500, x * 14000, Cys, Lys, His, andArg residues essential for catalysis | Klebsiella pneumoniae |
15500 | - |
F consisits of one polypeptide, S of at least four subunits, x * 60000, x * 23000, x * 15500, x * 14000, Cys, Lys, His, andArg residues essential for catalysis | Klebsiella pneumoniae |
19173 | - |
x * 28071, x * 60348, x * 24113, x * 19173, estimated from nucleotide sequences of cloned genes | Klebsiella pneumoniae |
23000 | - |
F consisits of one polypeptide, S of at least four subunits, x * 60000, x * 23000, x * 15500, x * 14000, Cys, Lys, His, andArg residues essential for catalysis | Klebsiella pneumoniae |
24113 | - |
x * 28071, x * 60348, x * 24113, x * 19173, estimated from nucleotide sequences of cloned genes | Klebsiella pneumoniae |
26000 | - |
1 * 26000, 1 * 200000, components F, S | Klebsiella pneumoniae |
28071 | - |
x * 28071, x * 60348, x * 24113, x * 19173, estimated from nucleotide sequences of cloned genes | Klebsiella pneumoniae |
60000 | - |
F consisits of one polypeptide, S of at least four subunits, x * 60000, x * 23000, x * 15500, x * 14000, Cys, Lys, His, andArg residues essential for catalysis | Klebsiella pneumoniae |
60348 | - |
x * 28071, x * 60348, x * 24113, x * 19173, estimated from nucleotide sequences of cloned genes | Klebsiella pneumoniae |
200000 | - |
1 * 26000, 1 * 200000, components F, S | Klebsiella pneumoniae |
220000 | 240000 | gel filtration, sedimentation equilibrium of cell-free extracts | Klebsiella pneumoniae |
250000 | - |
gel filtration of membrane extracts | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Klebsiella pneumoniae | production of both electron acceptor and intermediate for fermentative oxidation of 1,2-diols | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium sp. | - |
- |
- |
Klebsiella pneumoniae | - |
also Klebsiella oxytoca | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella pneumoniae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
propane-1,2-diol = propanal + H2O | general mechanism and stereochemistry, role of coenzyme in OH-group transfer, mechanism of overall reaction, role of free radicals during reaction, studies with coenzyme analogues | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-propanediol | - |
Salmonella enterica subsp. enterica serovar Typhimurium | propanal | - |
? | |
1,2-propanediol | - |
Klebsiella pneumoniae | propanal | - |
? | |
1,2-propanediol | - |
Flavobacterium sp. | propanal | - |
? | |
additional information | production of both electron acceptor and intermediate for fermentative oxidation of 1,2-diols | Klebsiella pneumoniae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | 1 * 26000, 1 * 200000, components F, S | Klebsiella pneumoniae |
More | F consisits of one polypeptide, S of at least four subunits, x * 60000, x * 23000, x * 15500, x * 14000, Cys, Lys, His, andArg residues essential for catalysis | Klebsiella pneumoniae |
oligomer | x * 28071, x * 60348, x * 24113, x * 19173, estimated from nucleotide sequences of cloned genes | Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cobamide | - |
Flavobacterium sp. | |
cobamide | slow inactivation by analogues | Klebsiella pneumoniae |