Activating Compound | Comment | Organism | Structure |
---|---|---|---|
betaine | activates, effects on wild-type and mutant enzymes, overview | Homo sapiens | |
delta-aminolevulinic acid | activates, effects on wild-type and mutant enzymes, overview | Homo sapiens | |
glycerol | activates, effects on wild-type and mutant enzymes, overview | Homo sapiens | |
additional information | taurine activates some cystathionine beta-synthase mutants slightly, while it slightly inhibits the wild-type enzyme and other cystathionine beta-synthase mutants | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A114V | site-directed mutagenesis, mutation at the dimer-dimer interface, the mutant shows a decrease in specific activity compared to the wild-type enzyme | Homo sapiens |
C165Y | site-directed mutagenesis, the mutant shows an increase in specific activity compared to the wild-type enzyme | Homo sapiens |
D444N | site-directed mutagenesis, the mutant shows altered activity compared to the wild-type enzyme | Homo sapiens |
E144K | site-directed mutagenesis, inactive mutant | Homo sapiens |
E176K | site-directed mutagenesis, the mutant shows a decrease in specific activity compared to the wild-type enzyme | Homo sapiens |
E302K | site-directed mutagenesis, the mutant shows altered activity compared to the wild-type enzyme | Homo sapiens |
G148R | site-directed mutagenesis, inactive mutant | Homo sapiens |
G305R | site-directed mutagenesis, inactive mutant | Homo sapiens |
G307S | site-directed mutagenesis, inactive mutant | Homo sapiens |
H65R | site-directed mutagenesis, inactive mutant | Homo sapiens |
I278T | site-directed mutagenesis, inactive mutant | Homo sapiens |
I435T | site-directed mutagenesis, the mutant shows an increase in specific activity compared to the wild-type enzyme | Homo sapiens |
K102N | site-directed mutagenesis, the mutant shows a decrease in specific activity compared to the wild-type enzyme | Homo sapiens |
L539S | site-directed mutagenesis, inactive mutant, the mutant shows altered activity compared to the wild-type enzyme | Homo sapiens |
additional information | effects of delta-aminolevulinic acid, betaine, glycerol and taurine on amounts of tetramers/oligomers of the cystathionine beta-synthase mutants, overview | Homo sapiens |
N228K | site-directed mutagenesis, inactive mutant | Homo sapiens |
P422L | site-directed mutagenesis, mutation in the regulatory domain, the mutant shows an increase in specific activity compared to the wild-type enzyme | Homo sapiens |
P49L | site-directed mutagenesis | Homo sapiens |
P78R | site-directed mutagenesis, mutation at the dimer-dimer interface | Homo sapiens |
R125Q | site-directed mutagenesis, the mutant shows a decrease in specific activity compared to the wild-type enzyme | Homo sapiens |
R266K | site-directed mutagenesis, inactive mutant | Homo sapiens |
R369C | site-directed mutagenesis, the mutant shows a decrease in specific activity compared to the wild-type enzyme | Homo sapiens |
R439Q | site-directed mutagenesis, the mutant shows altered activity compared to the wild-type enzyme | Homo sapiens |
S466L | site-directed mutagenesis, mutation in the regulatory domain, the mutant shows an increase in specific activity compared to the wild-type enzyme | Homo sapiens |
T191M | site-directed mutagenesis, inactive mutant | Homo sapiens |
T262R | site-directed mutagenesis, inactive mutant | Homo sapiens |
V180A | site-directed mutagenesis, mutation at the dimer-dimer interface, the mutant shows a decrease in specific activity compared to the wild-type enzyme | Homo sapiens |
W409_G453del | site-directed mutagenesis, inactive mutant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | taurine activates some cystathionine beta-synthase mutants slightly, while it slightly inhibits the wild-type enzyme and other cystathionine beta-synthase mutants | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + L-homocysteine | Homo sapiens | - |
L-cystathionine + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activities of wild-type and mutants in presence of activators, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + L-homocysteine | - |
Homo sapiens | L-cystathionine + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
CBS | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | role of heme in cystathionine beta-synthase folding | Homo sapiens | |
pyridoxal 5'-phosphate | - |
Homo sapiens |