Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.22 extracted from

  • Hnizda, A.; Spiwok, V.; Jurga, V.; Kozich, V.; Kodicek, M.; Kraus, J.P.
    Cross-talk between the catalytic core and the regulatory domain in cystathionine beta-synthase: study by differential covalent labeling and computational modeling (2010), Biochemistry, 49, 10526-10534.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
S-adenosyl-L-methionine allosteric activator. Binding of AdoMet to wild-type CBS moderately increases the protein stability toward urea, while it does not influence the unfolding cooperativity Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant 45CBS and wild-type CBS in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information generation of a truncated 45 kDa CBS, 45CBS, enzyme lacking the C-terminal regulatory domain, amino acids 1-413. The wild-type CBS exhibits lower resistance to urea-induced denaturation and lower degree of unfolding cooperativity compared to 45CBS. Proteolytic kinetics by thermolysin under native conditions reveals slower cleavage of wild-type CBS compared to the mutant 45CBS Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
4 * 61000 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine + L-homocysteine Homo sapiens
-
L-cystathionine + H2O
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant mutant 45CBS and wild-type CBS from Escherichia coli to homogeneity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + L-homocysteine
-
Homo sapiens L-cystathionine + H2O
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 61000 Homo sapiens
More CBS protein comprises three regions: the N-terminal heme-binding domain, residues 1-69, a highly conserved catalytic core, residues 70-413, and the C-terminal regulatory domain, resdiues 414-551, and an autoinhibitory module with binding site for the allosteric activator, S-adenosyl-L-methionine. Computational modeling of CBS structure, the enzyme has a regulatory dimer-dimer interface, homology modeling and protein-protein docking, overview. Model of wild-type CBS dimer by docking of a single C-terminal regulatory domain to mutant 45CBS dimer, PDB ID 1JBQ, and surface mapping of wild-type CBS and mutant 45CBS with diverse differentially reactive amino acid residues involved in conformational changes and thermal activation, overview Homo sapiens

Synonyms

Synonyms Comment Organism
CBS
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
wild-type CBS is therminally activated at 55°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Homo sapiens

General Information

General Information Comment Organism
malfunction CBS deficiency due to missense mutations in the CBS gene is the most common cause of inherited homocystinuria, a treatable multisystemic disease affecting to various extent vasculature, connective tissues, and central nervous system Homo sapiens
metabolism cystathionine beta-synthase is a pyridoxal 5'-phosphate-dependent enzyme, which catalyzes the first step of the transsulfuration pathway, namely, the condensation of serine with homocysteine to cystathionine Homo sapiens
additional information CBS is a modular enzyme, cross-talk between the catalytic core and the regulatory domain in cystathionine beta-synthase: study by differential covalent labeling and computational modeling, overview Homo sapiens
physiological function regulation mechanisms, overview Homo sapiens