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Literature summary for 4.2.1.22 extracted from

  • Gupta, S.; Wang, L.; Hua, X.; Krijt, J.; Kozich, V.; Kruger, W.D.
    Cystathionine beta-synthase p.S466L mutation causes hyperhomocysteinemia in mice (2008), Hum. Mutat., 29, 1048-1054.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
S-adenosyl-L-methionine about 4fold increase of specific activity in the presence of 0.25 mM S-adenosyl-L-methionine Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
wild type enzyme is expressed in Mus musculus and mutant enzyme S466L is expressed in Saccharomyces cerevisiae strain WY35 and in Mus musculus Homo sapiens

Protein Variants

Protein Variants Comment Organism
S466L the mutation causes hyperhomocysteinemia by affecting both the steady state level of CBS protein and by reducing the efficiency of the enzyme in vivo, S466L is enzymatically active, forms tetramers, and lacks S-adenosyl-L-methionine inducibility Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.77
-
L-serine mutant enzyme S466L, in the presence of 0.25 mM S-adenosyl-L-methionine Homo sapiens
0.78
-
L-serine wild type enzyme, in the presence of 0.25 mM S-adenosyl-L-methionine Homo sapiens
1.1
-
L-homocysteine mutant enzyme S466L, in the presence of 0.25 mM S-adenosyl-L-methionine Homo sapiens
2.5
-
L-homocysteine wild type enzyme, in the presence of 0.25 mM S-adenosyl-L-methionine Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-
kidney
-
Homo sapiens
-
liver highest activity Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + L-homocysteine
-
Homo sapiens L-cystathionine + H2O
-
?

Synonyms

Synonyms Comment Organism
CBS
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Homo sapiens