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Literature summary for 4.2.1.22 extracted from

  • Ozaki, S.; Inada, A.; Sada, K.
    Modulation of cystathionine beta-synthase activity by the Arg-51 and Arg-224 mutations (2008), Biosci. Biotechnol. Biochem., 72, 2318-2323.
    View publication on PubMed
Search for more literature for 4.2.1.22

Cloned(Commentary)

Cloned (Comment) Organism
full-length CBS and truncated enzyme containing residues 1-397 are expressed in Escherichia coli BL21 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
R224A the mutation decreases CBS activity by approximately 50% Homo sapiens
R51A the mutation decreases CBS activity by approximately 50% Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P35520
-
-

Purification (Commentary)

Purification (Comment) Organism
GST Trap FF column chromatography and Mono Q column chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2
-
 truncated ferrous mutant enzyme R51A, at 37°C and pH 8 Homo sapiens
1.3
-
 full-length ferrous mutant enzyme R224A, at 37°C and pH 8 Homo sapiens
1.3
-
 full-length ferrous mutant enzyme R51A, at 37°C and pH 8 Homo sapiens
2.2
-
 full-length ferric mutant enzyme R224A, at 37°C and pH 8 Homo sapiens
2.2
-
 full-length ferric mutant enzyme R51A, at 37°C and pH 8 Homo sapiens
2.8
-
 full-length ferrous wild type enzyme, at 37°C and pH 8 Homo sapiens
3.8
-
 truncated ferrous mutant enzyme R224A, at 37°C and pH 8 Homo sapiens
4
-
 truncated ferric mutant enzyme R51A, at 37°C and pH 8 Homo sapiens
5.2
-
 truncated ferric wild type enzyme, at 37°C and pH 8 Homo sapiens
6
-
 truncated ferric mutant enzyme R224A, at 37°C and pH 8 Homo sapiens
8
-
 truncated ferrous wild type enzyme, at 37°C and pH 8 Homo sapiens
10.3
-
 full-length ferric wild type enzyme, at 37°C and pH 8 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + L-homocysteine
-
 Homo sapiens L-cystathionine + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CBS
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
heme the specific activity of CBS improves 1.8-2.8fold under truncation of R51A and R224A when the heme iron is in the ferric state Homo sapiens
pyridoxal 5'-phosphate
-
 Homo sapiens