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Literature summary for 4.2.1.22 extracted from

  • Bukovska, G.; Kery, V.; Kraus, J.P.
    Expression of human cystathionine beta-synthase in Escherichia coli: purification and characterization (1994), Protein Expr. Purif., 5, 442-448.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
homocysteine absence of S-adenosyl-L-methionine Homo sapiens
3.1
-
L-Ser absence of S-adenosyl-L-methionine Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
high tendency for aggregation Homo sapiens
63000
-
x * 63000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-Serine + homocysteine Homo sapiens
-
?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-70°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Serine + homocysteine ir Homo sapiens Cystathionine + H2O
-
?
L-Serine + homocysteine
-
Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
? x * 63000, SDS-PAGE Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate requirement Homo sapiens
S-adenosyl-L-methionine allosterical regulation, increase of the enzyme's affinity for homocysteine Homo sapiens