Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
A71G | mutation in alpha-subunit, about 10fold decrease in kcat/Km value compared to wild-type alpha subunit | Escherichia coli |
T183V | substitution decreases catalytic efficiency of the alpha-subunit in the absence of the beta subunit, leading to local changes in the structural dynamics of the beta2alpha2 and beta6alpha6 loops | Escherichia coli |
T183V/A158G | mutation in alpha-subunit, about 12fold decrease in kcat/Km value compared to wild-type alpha subunit | Escherichia coli |
T183V/A180G | mutation in alpha-subunit, about 20fold decrease in kcat/Km value compared to wild-type alpha subunit | Escherichia coli |
T183V/A185G | mutation in alpha-subunit, about 12fold decrease in kcat/Km value compared to wild-type alpha subunit | Escherichia coli |
T183V/A59G | mutation in alpha-subunit, about 50fold decrease in kcat/Km value compared to wild-type alpha subunit | Escherichia coli |
T183V/A67G | mutation in alpha-subunit, about 30fold decrease in kcat/Km value compared to wild-type alpha subunit | Escherichia coli |
T183V/A71G | mutation in alpha-subunit, about 8fold decrease in kcat/Km value compared to wild-type alpha subunit | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.78 | - |
indole | alpha-subunit, mutant T183V/A185G, pH 7.6, 25°C | Escherichia coli | |
0.8 | - |
indole | alpha-subunit, mutant T183V/A158G, pH 7.6, 25°C | Escherichia coli | |
0.84 | - |
indole | alpha-subunit, mutant T183V/A67G, pH 7.6, 25°C | Escherichia coli | |
0.926 | - |
indole | wild-type, pH 7.6, 25°C | Escherichia coli | |
1.1 | - |
indole | alpha-subunit, mutant T183V, pH 7.6, 25°C | Escherichia coli | |
1.5 | 2 | indole | alpha-subunit, mutant T183V/A180G, pH 7.6, 25°C | Escherichia coli | |
1.7 | - |
indole | alpha-subunit, mutant T183V/A71G, pH 7.6, 25°C | Escherichia coli | |
1.84 | - |
indole | alpha-subunit, mutant A71G, pH 7.6, 25°C | Escherichia coli | |
3.35 | - |
indole | alpha-subunit, mutant T183V/A59G, pH 7.6, 25°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A877 | alpha-chain | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + indole | - |
Escherichia coli | 1-(indol-3-yl)glycerol 3-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
indole | alpha-subunit, mutant T183V/A71G, pH 7.6, 25°C | Escherichia coli | |
0.007 | - |
indole | alpha-subunit, mutant T183V, pH 7.6, 25°C | Escherichia coli | |
0.007 | - |
indole | alpha-subunit, mutant T183V/A158G, pH 7.6, 25°C | Escherichia coli | |
0.007 | - |
indole | alpha-subunit, mutant T183V/A180G, pH 7.6, 25°C | Escherichia coli | |
0.007 | - |
indole | alpha-subunit, mutant T183V/A185G, pH 7.6, 25°C | Escherichia coli | |
0.01 | - |
indole | alpha-subunit, mutant T183V/A59G, pH 7.6, 25°C | Escherichia coli | |
0.011 | - |
indole | alpha-subunit, mutant T183V/A67G, pH 7.6, 25°C | Escherichia coli | |
0.02 | - |
indole | alpha-subunit, mutant A71G, pH 7.6, 25°C | Escherichia coli | |
0.095 | - |
indole | alpha-subunit, wild-type, pH 7.6, 25°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00176 | - |
indole | alpha-subunit, mutant T183V/A71G, pH 7.6, 25°C | Escherichia coli | |
0.00299 | - |
indole | alpha-subunit, mutant T183V/A59G, pH 7.6, 25°C | Escherichia coli | |
0.00461 | - |
indole | alpha-subunit, mutant T183V/A180G, pH 7.6, 25°C | Escherichia coli | |
0.00636 | - |
indole | alpha-subunit, mutant T183V, pH 7.6, 25°C | Escherichia coli | |
0.00871 | - |
indole | alpha-subunit, mutant T183V/A158G, pH 7.6, 25°C | Escherichia coli | |
0.00895 | - |
indole | alpha-subunit, mutant T183V/A185G, pH 7.6, 25°C | Escherichia coli | |
0.0109 | - |
indole | alpha-subunit, mutant A71G, pH 7.6, 25°C | Escherichia coli | |
0.0131 | - |
indole | alpha-subunit, mutant T183V/A67G, pH 7.6, 25°C | Escherichia coli | |
0.103 | - |
indole | wild-type, pH 7.6, 25°C | Escherichia coli |